Bu-1b antibody

Principal name

Bu-1b antibody

Alternative names for Bu-1b antibody

B6.2, chicken B-cell marker

SwissProt ID

Q90747 (Chick)

Ncbi ID

9031

Available reactivities

Chk (Chicken)

Available hosts

Mouse

Available applications

Frozen Sections (C), Immunoprecipitation (IP), Flow Cytometry (F), Paraffin Sections (P)

Background of Bu-1b antibody

Bu1b is expressed on chicken B cells throughout most of their development, as well as on some non lymphoid cells. It has no recognizable similarity to known mammalian molecules and therefore represents a unique B cell marker. Its presence in chickens may be related to differences in the properties of B cell development between chickens and mammalian species. Chicken Bu1b, a product of the Bu1b allele, is expressed on the cell surface as a disulfide linked homodimer. In 10 week old H.B15 and H.B2 chickens, Bu1 is found on 85 to 90% of bursal cells, 2 to 8% of thymocytes, 15 to 27% of spleen cells, and 2 to 18% of peripheral blood cells. It is also expressed on subsets of macrophages and monocytes, but not on granulocytes, erythrocytes or thrombocytes. In chickens heterozygous for the Bu1 alleles (Bu1 a/b), Bu1a does not exhibit allelic exclusion.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Bu-1b antibody

Bu-1b Mouse IgG1 5-11G2 Biotin Chk F
0.5 mg / €410.00
  Acris Antibodies GmbH

Bu-1b antibody

Bu-1b Mouse IgG1 5-11G2 FITC Chk F
0.5 mg / €450.00
  Acris Antibodies GmbH

Bu-1b antibody

Immunofluorescent Staining: Chicken peripheral blood mononuclear cells were first incubated with purified Mouse anti-Chicken Bu-1b and then counter-stained with Goat anti-Mouse IgG-FITC. Small lymphocytes were then gated using light scatter criterias and analyzed on a FACScan(TM) flow cytometer (BDIS, San Jose, CA). Amount Used: 1 µg/10e6 cells. Mouse IgG1 5-11G2 Purified Chk C, F, IP
0.5 mg / €340.00
  Acris Antibodies GmbH

Bu-1b antibody

Bu-1b Mouse IgG1 5-11G2 PE Chk F
0.1 mg / €320.00
  Acris Antibodies GmbH

Bu-1b antibody

Chicken peripheral blood mononuclear cells were first incubated with Chicken Bu-1b monoclonal antibody, clone 5-11G2 ( Cat # MAB3276 ) and then counter-stained with goat anti-mouse IgG-FITC. Small lymphocytes were gated using lignt scatter criteria and analyzed on a FACScanTM flow cytometer ( BDB, San Jose, CA ). Mouse IgG1 5-11G2 Chk C, F, IP
0.5 mg / €390.00
  Abnova Taiwan Corp.

Bu-1b antibody

Flow cytometry: Bu-1b Antibody (5-11G2) [NBP1-28323] - 1 ug/10^6 Chicken peripheral blood mononuclear cells were first incubated with purified mouse anti-chicken Bu-1b and then counter-stained with goat anti-mouse IgG-FITC. Small lymphocytes were gated using light scatter criteria and analyzed on a flow cytometer Mouse IgG1 5-11G2 Purified Chk C, F, IP, P
0.5 mg / €470.00
  Novus Biologicals Inc.

Bu-1b antibody

Flow cytometry / FACS analysis: Bu-1b Antibody (5-11G2) [FITC] [NBP1-28324] - Chicken peripheral blood mononuclear cells were first incubated with purified mouse anti-chicken Bu-1b and then counter-stained with goat anti-mouse IgG-FITC. Small lymphocytes were gated using lignt scatter criteria and analyzed on a FACScan® flow cytometer (BDB, San Jose, CA). Mouse IgG1 5-11G2 FITC Chk C, F, IP, P
0.25 mg / €600.00
  Novus Biologicals Inc.

Bu-1b antibody

Flow cytometry / FACS analysis: Bu-1b Antibody (5-11G2) [Biotin] [NBP1-28325] - Chicken peripheral blood mononuclear cells were first incubated with purified mouse anti-chicken Bu-1b and then counter-stained with goat anti-mouse IgG-FITC. Small lymphocytes were gated using lignt scatter criteria and analyzed on a FACScan® flow cytometer (BDB, San Jose, CA). Mouse IgG1 5-11G2 Biotin Chk C, F, IP, P
0.25 mg / €600.00
  Novus Biologicals Inc.

8 Item(s)

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