BUB1A antibody

Principal name

BUB1A antibody

Alternative names for BUB1A antibody

BUB1L, Mitotic checkpoint serine/threonine-protein kinase BUB1

SwissProt ID

O08901 (Mouse), O43683 (Human)

Gene ID

669 (BPGM), 699 (BUB1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Kangaroo, Xen (Xenopus)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Flow Cytometry (F)

Background of BUB1A antibody

Chromosome segregation and mitotic checkpoints are regulated by various proteins among them are BUB1 and BUBR1/BUB1B (Budding Uninhibited by Benzimidazoles 1). During mitosis both BUB1 and BUBR1 proteins are localized to kinetochores and inhibit the anaphase stage until all chromosomes are correctly attached to the spindle. These proteins were found due to their homology to Bub1 protein from yeast. Human BUBR1 contains CD1 and CD2 domains that are found in human, mouse and yeast BUB1.3-5 The human and mouse BUBR1 proteins are 29% identical in these regions. The kinetochore localization and Bub3 binding is found in the CD1 region while the kinase domain is found in the CD2 domain. While both human and mouse BUB1 contain a nuclear localization site between CD1 and CD2 regions, the yeast protein does not contain this site. BUB1 is expressed in many tissues with the highest expression in testis and thymus, lower expression in colon and small intestine and very low expression in spleen and ovary. BUB1 expression is in correlation with the expression of MAD2, another human spindle assembly checkpoint protein.3-5 BUBR1 is mainly expressed in testis, thymus and bone marrow while in differentiated tissues such as brain, heart and muscle the expression is low.5

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

BUB1A antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between BUB1 and APC. HeLa cells were stained with anti-BUB1 rabbit purified polyclonal 1:1200 and anti-APC mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Rabbit Purified Hu, Ms E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

BUB1A (N-term) antibody

Western blot analysis of bub1 in A-20 lysate with bub1 antibody at (A) 2 and (B) 4 ug/mL. Rabbit IgG Aff - Purified Hu, Ms E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

BUB1A antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using BUB1 antibody.The picture on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

BUB1A antibody

Western blot analysis of extracts from COLO205 cells, using BUB1 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

BUB1A antibody

Western blot analysis of extracts of HeLa cell line , using BUB1 antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

BUB1A antibody

Western blot analysis of BUB1 expression in COLO205 cells Rabbit IgG Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

BUB1A antibody

Small intestine: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu, Ms P, WB
50 µg / €470.00
  Acris Antibodies GmbH

BUB1A (C-term) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

BUB1A Control Peptide

BUB1A
50 µg / €160.00
  Acris Antibodies GmbH

BUB1A Control Peptide

BUB1A
50 µg / €160.00
  Acris Antibodies GmbH

BUB1A antibody

BUB1A Mouse IgG1 14H5 Purified Hu ICC/IF, IP, WB
50 µg / €380.00
  Acris Antibodies GmbH

BUB1A antibody

Western Blot analysis of BUB1 expression in transfected 293T cell line (H00000699-T01) by BUB1 MaxPab polyclonal antibody.

Lane1:BUB1 transfected lysate(119.35 KDa).
Lane2:Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

BUB1A antibody

BUB1A Mouse Hu WB
50 µg / €370.00
  Abnova Taiwan Corp.

BUB1A antibody

Immunofluorescence of monoclonal antibody to BUB1 on HeLa cell . [antibody concentration 10 ug/ml] Mouse IgG1 4C3-4C9 Purified Hu E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

BUB1A antibody

BUB1 monoclonal antibody (M02), clone 2F9 Western Blot analysis of BUB1 expression in HeLa NE ( Cat # L013V3 ). Mouse IgG1 2F9 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

BUB1A antibody

BUB1A Mouse IgG1 2F9 Ascites Hu E, WB
0.2 ml / €370.00
  Abnova Taiwan Corp.

BUB1A antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with BUB1 polyclonal antibody ( Cat # PAB3262 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma. Rabbit Hu E, P, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

BUB1A antibody

Immunocytochemistry of BUB1 in A-20 cells with BUB1 polyclonal antibody ( Cat # PAB13270 ) at 10 µg/ml. Rabbit Hu, Ms ICC/IF, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

BUB1A antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using BUB1 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, P
50 µg / €205.00
  Assay Biotech

BUB1A antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using BUB1 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, P
0.1 mg / €310.00
  Assay Biotech

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