BUB1A antibody

Principal name

BUB1A antibody

Alternative names for BUB1A antibody

BUB1L, Mitotic checkpoint serine/threonine-protein kinase BUB1

SwissProt ID

O08901 (Mouse), O43683 (Human)

Gene ID

699 (BUB1), 12235 (Bub1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Paraffin Sections (P), ELISA (detection) (E(detection))

Background of BUB1A antibody

The Mitotic Checkpoint Complex (MCC), which contains Bub1, Bub1b, Bub3, Mad2, and Cdc20, controls chromosome segregation and monitors kinetochore-microtubule interactions. During mitosis, the MCC complex inhibits the ubiquitin ligase activity of the Aphase Promoting Complex/Cyclosome (APC/C), thereby preventing cells with uligned chromosomes from prematurely entering aphase. Research studies have shown that Bub1b and Bub1 kises are mutated in several types of human maligncies including hematopoietic, colorectal, lung, and breast cancers. Biallelic mutations in Bub1b have been found in mosaic variegated aneuploidy syndrome and premature chromatid separation syndrome. Bub1b mouse germline knockouts are embryonic lethal with heterozygous animals displaying genetic instability, early aging phenotypes, and increased cancer susceptibility. Bub3 binds both Bub1 and Bub1b, facilitating their recruitment to kinetochores, and is required for functiol microtubule-kinetochore interactions.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

BUB1A antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between BUB1 and APC. HeLa cells were stained with anti-BUB1 rabbit purified polyclonal 1:1200 and anti-APC mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Rabbit Purified Hu, Ms E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

BUB1A (N-term) antibody

Western blot analysis of bub1 in A-20 lysate with bub1 antibody at (A) 2 and (B) 4 ug/mL. Rabbit IgG Aff - Purified Hu, Ms E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

BUB1A antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using BUB1 antibody.The picture on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

BUB1A antibody

Western blot analysis of extracts from COLO205 cells, using BUB1 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

BUB1A antibody

Western blot analysis of extracts of HeLa cell line , using BUB1 antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

BUB1A antibody

Western blot analysis of BUB1 expression in COLO205 cells Rabbit IgG Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

BUB1A antibody

BUB1A Rabbit IgG Purified Hu E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

BUB1A antibody

Small intestine: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu, Ms P, WB
50 µg / €470.00
  Acris Antibodies GmbH

BUB1A (C-term) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

BUB1A Control Peptide

BUB1A
50 µg / €160.00
  Acris Antibodies GmbH

BUB1A Control Peptide

BUB1A
50 µg / €160.00
  Acris Antibodies GmbH

BUB1A antibody

BUB1A Mouse IgG1 14H5 Purified Hu ICC/IF, IP, WB
50 µg / €380.00
  Acris Antibodies GmbH

BUB1A antibody

Western Blot analysis of BUB1 expression in transfected 293T cell line (H00000699-T01) by BUB1 MaxPab polyclonal antibody.

Lane1:BUB1 transfected lysate(119.35 KDa).
Lane2:Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

BUB1A antibody

BUB1A Mouse Hu WB
50 µg / €370.00
  Abnova Taiwan Corp.

BUB1A antibody

Immunofluorescence of monoclonal antibody to BUB1 on HeLa cell . [antibody concentration 10 ug/ml] Mouse IgG1 4C3-4C9 Purified Hu E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

BUB1A antibody

BUB1 monoclonal antibody (M02), clone 2F9 Western Blot analysis of BUB1 expression in HeLa NE ( Cat # L013V3 ). Mouse IgG1 2F9 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

BUB1A antibody

BUB1A Mouse IgG1 2F9 Ascites Hu E, WB
0.2 ml / €370.00
  Abnova Taiwan Corp.

BUB1A antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with BUB1 polyclonal antibody ( Cat # PAB3262 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma. Rabbit Hu E, P, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

BUB1A antibody

Immunocytochemistry of BUB1 in A-20 cells with BUB1 polyclonal antibody ( Cat # PAB13270 ) at 10 µg/ml. Rabbit Hu, Ms ICC/IF, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

BUB1A antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using BUB1 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, P
50 µg / €205.00
  Assay Biotech

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