BUB1B antibody

Principal name

BUB1B antibody

Alternative names for BUB1B antibody

Protein SSK1, BUBR1, MAD3L, BUB1 beta, Mitotic checkpoint serine/threonine-protein kinase BUB1 beta, MAD3/BUB1-related protein kinase, Mitotic checkpoint kinase MAD3L

SwissProt ID

O60566 (Human), Q59HH6 (Human), Q9Z1S0 (Mouse)

Gene ID

701 (BUB1B)

Available reactivities

Hu (Human), Ms (Mouse), Mky (Monkey), Can (Canine), Rt (Rat)

Available hosts

Mouse, Rabbit, Sheep

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Frozen Sections (C), ELISA (detection) (E(detection)), Flow Cytometry (F), Luminex (LMNX)

Background of BUB1B antibody

The Mitotic Checkpoint Complex (MCC), which contains Bub1, Bub1b, Bub3, Mad2, and Cdc20, controls chromosome segregation and monitors kinetochore-microtubule interactions . During mitosis, the MCC complex inhibits the ubiquitin ligase activity of the Aphase Promoting Complex/Cyclosome (APC/C), thereby preventing cells with uligned chromosomes from prematurely entering aphase . Research studies have shown that Bub1b and Bub1 kises are mutated in several types of human maligncies including hematopoietic, colorectal, lung, and breast cancers . Biallelic mutations in Bub1b have been found in mosaic variegated aneuploidy syndrome and premature chromatid separation syndrome . Bub1b mouse germline knockouts are embryonic lethal with heterozygous animals displaying genetic instability, early aging phenotypes, and increased cancer susceptibility . Bub3 binds both Bub1 and Bub1b, facilitating their recruitment to kinetochores , and is required for functiol microtubule-kinetochore interactions .

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

BUB1B antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of lung tissue using anti-BUB1Bmouse monoclonal antibody. (Clone UMAB7, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min) Mouse IgG1 UMAB7 Purified Hu, Mky F, ICC/IF, P, WB
0.1 ml / €468.00
  OriGene Technologies, Inc.

UltraMAB

BUB1B antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of lung tissue using anti-BUB1Bmouse monoclonal antibody. (Clone UMAB7, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min) Mouse IgG1 UMAB7 Purified Hu, Mky F, ICC/IF, P, WB
30 µl / €140.00
  OriGene Technologies, Inc.

UltraMAB

BUB1B antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of pancreas tissue using anti-BUB1Bmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500533, Dilution 1:50) Mouse IgG1 OTI5D9 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

BUB1B antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of kidney tissue using anti-BUB1Bmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500534, Dilution 1:50) Mouse IgG1 OTI3C7 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

BUB1B antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-BUB1B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500535, Dilution 1:50) Mouse IgG1 OTI10B7 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

BUB1B antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of thyroid tissue using anti-BUB1Bmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500540, Dilution 1:50) Mouse IgG1 OTI11G2 Purified Hu, Mky ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

BUB1B antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-BUB1Bmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500541, Dilution 1:50) Mouse IgG1 OTI11D7 Purified Hu, Mky ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

BUB1B antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-BUB1Bmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500586, Dilution 1:50) Mouse IgG1 OTI15E11 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

BUB1B antibody

Immunohistochemical staining of paraffin-embedded breast tissue within the normal limits using anti-BUB1B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500606, Dilution 1:50) Mouse IgG1 OTI10E7 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

BUB1B antibody

Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-BUB1Bmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500607, Dilution 1:50) Mouse IgG1 OTI6E5 Purified Hu F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

BUB1B antibody

Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-BUB1Bmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500609, Dilution 1:50) Mouse IgG2b OTI3D9 Purified Hu F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

BUB1B antibody

Anti-BUB1B mouse monoclonal antibody (TA500679) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BUB1B(RC205844). Mouse IgG1 OTI6E3 Purified Can, Hu, Mky, Rt F, ICC/IF, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

BUB1B antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-BUB1B Rabbit Polyclonal antibody. (TA590739) Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

BUB1B antibody

BUB1B ELISA with 3C7 Capture (TA600521) and 6E5 Detection (TA700531) Antibodies. Substrate used: Recombinant Human BUB1B (TP305844) Mouse IgG1 OTI3C7 Purified Hu E, LMNX
0.1 mg / €325.00
  OriGene Technologies, Inc.

BUB1B antibody

Immunoperoxidase of monoclonal BUB1B antibody on Formalin-Fixed Paraffin-Embedded Human spleen at 3 µg/ml. Mouse IgG 2G5 Purified Hu E, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

BUB1B antibody

Immunoperoxidase of monoclonal antibody to BUB1B on formalin-fixed paraffin-embedded human spleen. [antibody concentration 3 ug/ml] Mouse IgG 2G5 Purified Hu E, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

BUB1B antibody

Immunohistochemistry-Paraffin: BubR1 Antibody (6E5) [NBP1-47680] - Immunohistochemical staining of paraffin-embedded Carcinoma of bladder tissue using anti-BUB1Bmouse monoclonal antibody. (Dilution 1:50) Mouse 6E5 Aff - Purified Hu F, ICC/IF, P, WB
0.1 ml / €530.00
  Novus Biologicals Inc.

BUB1B (N-term) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

BUB1B antibody

BUBR1 detected in HeLa cell lysate Mouse IgG1 8G1 Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

BUB1B antibody

Western blot analysis of extracts from HeLa cells and HepG2 cells all treated with H2O2 (100uM, 30mins), using BUB1B antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

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