BUB3 antibody

Principal name

BUB3 antibody

Alternative names for BUB3 antibody

Mitotic checkpoint protein BUB3

SwissProt ID

O43684 (Human), Q1JQB2 (Bovin), Q9WVA3 (Mouse)

Gene ID

9184 (BUB3)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Chk (Chicken), African clawed frog, Mky (Monkey), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Immunoprecipitation (IP)

Background of BUB3 antibody

We know how important it is to your research to have access to antibodies specific for your model. As it is often impossible to know which antibody will work best for a specific sample and application, we now offer SuperNovus Packs. These packs include different antibodies to the same protein to give you the ability to decide for yourself. The SuperNovus Packs are all offered at a discount price compared to purchasing each antibody individually. The spindle checkpoint ensures the fidelity of chromosome segregation in mitosis and meiosis. In response to defects in the mitotic apparatus, it blocks the activity of the anaphase-promoting complex, a large ubiquitin ligase required for chromosome segregation. Recent studies indicate that the spindle checkpoint monitors both the attachment of chromosomes to the mitotic spindle and the tension across the sister chromatid generated by microtubules. Upon checkpoint activation, checkpoint protein complexes containing BubR1(Mad3), Bub3, Mad2 and Cdc20 directly bind to the anaphase-promoting complex and inhibit its ligase activity. Therefore, the checkpoint proteins form a complex intracellular signalling network to inhibit the anaphase-promoting complex.

General readings

Loqarinho. E., et al. (2008) Mol Biol Cell 19(4): 1798-1813Warren. C.D., et al. (2002) Mol Biol Cell 13(9): 3029-3041Taylor. S.S., et al. (1998) J Cell Biol 142(1): 1-11

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

BUB3 (N-term) antibody

Western blot analysis of bub3 in Jurkat cell lysate with bub3 antibody at (A) 0.5 and (B) 1 ug/mL. Rabbit IgG Aff - Purified Hu, Ms E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

BUB3 antibody

WB Suggested Anti-BUB3 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: PANC1 cell lysate.BUB3 is strongly supported by BioGPS gene expression data to be expressed in PANC1 Rabbit IgG Purified Bov, Eq, GP, Hu, Ms, Por, Rb, Rt ICC/IF, WB
50 µg / €325.00
  OriGene Technologies, Inc.

BUB3 antibody

The cell lysates of HeLa(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BUB3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG2b AT2H6 Purified Hu E, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

BUB3 antibody

The cell lysates of HeLa(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BUB3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG2b AT2H6 Purified Hu E, WB
50 µl / €220.00
  Acris Antibodies GmbH

BUB3 antibody

Spleen: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu, Ms P, WB
50 µg / €470.00
  Acris Antibodies GmbH

BUB3 antibody

Immunohistochemistry analysis of Bub3 antibody (Cat.-No AP20475PU-N) in paraffin-embedded human brain tissue. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

BUB3 Control Peptide

BUB3
50 µg / €160.00
  Acris Antibodies GmbH

BUB3 antibody

Immunocytochemistry of BUB3 in Jurkat cells with BUB3 polyclonal antibody ( Cat # PAB12997 ) at 10 µg/ml. Rabbit Hu, Ms ICC/IF, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

BUB3 antibody

Western blot analysis of extracts from HeLa cells/Jurkat cells/293 cells, using BUB3 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €205.00
  Assay Biotech

BUB3 antibody

Western blot analysis of extracts from HeLa cells/Jurkat cells/293 cells, using BUB3 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €310.00
  Assay Biotech

BUB3 antibody

HCT116; . Sample Type :. HCT116. . Primary Antibody Dilution:. 4 ug/ml . Secondary Antibody :. Anti-rabbit Alexa 546 . Secondary Antibody Dilution:. 2 ug/ml . Gene Name :. BUB3; BUB3 antibody - N-terminal region (ARP58599_P050) in HCT116 cells using Immunofluorescence Rabbit Aff - Purified African clawed frog, Bov, Chk, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

BUB3 antibody

BUB3 Rabbit IgG Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €290.00
  Boster Immunoleader

BUB3 antibody

Immunohistochemical analysis of BUB3 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Mky ICC/IF, IP, P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

BUB3 antibody

Immunohistochemical analysis of BUB3 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Mky ICC/IF, IP, P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

BUB3 antibody

Immunohistochemical analysis of BUB3 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Mky ICC/IF, IP, P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

BUB3 (Super Novus Pack) antibody

Western Blot: BUB3 Antibodies SuperNovus Pack [NB100-919] - Detection of Human BUB3 by Western Blot. Samples: Whole cell lysate (50 mcg) from HeLa cells treated with Nocodazole (+) or mock treated (-). Antibody: Affinity purified rabbit anti-BUB3 antibody NB100-573 used at 0.1 mcg/ml. Detection: Chemiluminescence with an exposure time of 10 minutes. Rabbit Aff - Purified Hu WB
3 vials / €710.00
  Novus Biologicals Inc.

BUB3 antibody

Immunohistochemistry: Bub3 Antibody [NBP1-19630] -  Bub3 Antibody [NBP1-19630] - paraffin-embedded human brain tissue. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €420.00
  Novus Biologicals Inc.

BUB3 antibody

Immunohistochemistry: BUB3 Antibody [NBP1-33558] - Immunohistochemical analysis of BUB3 on paraffin-embedded A549 xenograft, using NBP1-33558. Rabbit IgG Hu P, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

BUB3 antibody

BUB3 Rabbit IgG Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €360.00
  Novus Biologicals Inc.

BUB3 antibody

BUB3 Rabbit IgG Aff - Purified Hu, Ms, Rt E, WB
50 µg / €290.00
  Novus Biologicals Inc.

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