BUB3 antibody

Principal name

BUB3 antibody

Alternative names for BUB3 antibody

Mitotic checkpoint protein BUB3

SwissProt ID

O43684 (Human), Q1JQB2 (Bovin), Q9WVA3 (Mouse)

Gene ID

9184 (BUB3)

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Chk (Chicken), Rt (Rat), African clawed frog, Mky (Monkey), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E), Immunoprecipitation (IP)

Background of BUB3 antibody

The mitotic checkpoint protein Bub3 is involved with the essential spindle checkpoint pathway which operates during early embryogenesis. Bub3 is important during G2 and early mitosis stages, permitting entry into mitosis depending upon the assembly state of microtubules, thus preventing premature sister chromatid separation, mis-segregation and aneuploidy. Bub3 contains four WD repeat domains and is required for the kinetochore localization of Bub1, a related kinase that is necessary for spindle assembly checkpoint function. Bub1 is able to autophosphorylate and can catalyze the phosphorylation of Bub3. Both Bub1 and Bub3 are mutually dependent for function. Altered Bub expression levels may significantly impair mitotic checkpoint function and is associated with tumor cell proliferation.

General readings

Loqarinho. E., et al. (2008) Mol Biol Cell 19(4): 1798-1813Warren. C.D., et al. (2002) Mol Biol Cell 13(9): 3029-3041Taylor. S.S., et al. (1998) J Cell Biol 142(1): 1-11

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

BUB3 (N-term) antibody

Western blot analysis of bub3 in Jurkat cell lysate with bub3 antibody at (A) 0.5 and (B) 1 ug/mL. Rabbit IgG Aff - Purified Hu, Ms E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

BUB3 antibody

Western blot analysis of extracts from HeLa cells, Jurkat cells and 293 cells, using BUB3 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

BUB3 antibody

WB Suggested Anti-BUB3 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: PANC1 cell lysate.BUB3 is strongly supported by BioGPS gene expression data to be expressed in PANC1 Rabbit IgG Purified Bov, Eq, GP, Hu, Ms, Por, Rb, Rt ICC/IF, WB
50 µg / €325.00
  OriGene Technologies, Inc.

BUB3 antibody

BUB3 Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

BUB3 antibody

The cell lysates of HeLa(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BUB3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG2b AT2H6 Purified Hu E, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

BUB3 antibody

The cell lysates of HeLa(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BUB3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG2b AT2H6 Purified Hu E, WB
50 µl / €220.00
  Acris Antibodies GmbH

BUB3 antibody

Spleen: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu, Ms P, WB
50 µg / €470.00
  Acris Antibodies GmbH

BUB3 antibody

Immunohistochemistry analysis of Bub3 antibody (Cat.-No AP20475PU-N) in paraffin-embedded human brain tissue. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

BUB3 Control Peptide

BUB3
50 µg / €160.00
  Acris Antibodies GmbH

BUB3 antibody

Immunocytochemistry of BUB3 in Jurkat cells with BUB3 polyclonal antibody ( Cat # PAB12997 ) at 10 µg/ml. Rabbit Hu, Ms ICC/IF, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

BUB3 antibody

Western blot analysis of extracts from HeLa cells/Jurkat cells/293 cells, using BUB3 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €205.00
  Assay Biotech

BUB3 antibody

Western blot analysis of extracts from HeLa cells/Jurkat cells/293 cells, using BUB3 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €310.00
  Assay Biotech

BUB3 antibody

HCT116; . Sample Type :. HCT116. . Primary Antibody Dilution:. 4 ug/ml . Secondary Antibody :. Anti-rabbit Alexa 546 . Secondary Antibody Dilution:. 2 ug/ml . Gene Name :. BUB3; BUB3 antibody - N-terminal region (ARP58599_P050) in HCT116 cells using Immunofluorescence Rabbit Aff - Purified African clawed frog, Bov, Chk, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

BUB3 antibody

BUB3 Rabbit IgG Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €290.00
  Boster Immunoleader

BUB3 antibody

Immunohistochemical analysis of BUB3 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Mky ICC/IF, IP, P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

BUB3 antibody

Immunohistochemical analysis of BUB3 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Mky ICC/IF, IP, P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

BUB3 antibody

Immunohistochemical analysis of BUB3 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Mky ICC/IF, IP, P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

BUB3 (transcript variant 2)

BUB3 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

BUB3 (1-328, His-tag)

BUB3 Human Purified > 85 % by SDS - PAGE E. coli
0.5 mg / €730.00
  Acris Antibodies GmbH

BUB3 (1-328, His-tag)

BUB3 Human Purified > 85 % by SDS - PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

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