BUB3 antibody

Principal name

BUB3 antibody

Alternative names for BUB3 antibody

Mitotic checkpoint protein BUB3

SwissProt ID

O43684 (Human), Q1JQB2 (Bovin), Q9WVA3 (Mouse)

Gene ID

9184 (BUB3)

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Chk (Chicken), Rt (Rat), African clawed frog, Mky (Monkey), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E), Immunoprecipitation (IP)

Background of BUB3 antibody

The mitotic checkpoint protein Bub3 is involved with the essential spindle checkpoint pathway which operates during early embryogenesis. Bub3 is important during G2 and early mitosis stages, permitting entry into mitosis depending upon the assembly state of microtubules, thus preventing premature sister chromatid separation, mis-segregation and aneuploidy. Bub3 contains four WD repeat domains and is required for the kinetochore localization of Bub1, a related kinase that is necessary for spindle assembly checkpoint function. Bub1 is able to autophosphorylate and can catalyze the phosphorylation of Bub3. Both Bub1 and Bub3 are mutually dependent for function. Altered Bub expression levels may significantly impair mitotic checkpoint function and is associated with tumor cell proliferation.

General readings

Loqarinho. E., et al. (2008) Mol Biol Cell 19(4): 1798-1813Warren. C.D., et al. (2002) Mol Biol Cell 13(9): 3029-3041Taylor. S.S., et al. (1998) J Cell Biol 142(1): 1-11

7 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

BUB3 (N-term) antibody

  Rabbit Polyclonal Bub3 Antibody  
Western blot analysis of bub3 in Jurkat cell lysate with bub3 antibody at (A) 0.5 and (B) 1 ug/mL. Rabbit IgG Aff - Purified Hu, Ms E, ICC/IF, WB
0.1 mg / €371.00
  OriGene Technologies, Inc.

BUB3 antibody

  Rabbit polyclonal anti-BUB3 antibody  
Western blot analysis of extracts from HeLa cells, Jurkat cells and 293 cells, using BUB3 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.

BUB3 antibody

  Rabbit Polyclonal Anti-BUB3 Antibody  
BUB3 Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.

BUB3 antibody

The cell lysates of HeLa(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BUB3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG2b AT2H6 Purified Hu E, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

BUB3 antibody

The cell lysates of HeLa(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BUB3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG2b AT2H6 Purified Hu E, WB
50 µl / €220.00
  Acris Antibodies GmbH

BUB3 antibody

Western blot analysis of extracts from HeLa cells/Jurkat cells/293 cells, using BUB3 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €230.00
  Assay Biotech

BUB3 antibody

Western blot analysis of extracts from HeLa cells/Jurkat cells/293 cells, using BUB3 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €330.00
  Assay Biotech

7 Item(s)

per page
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