c-fos antibody

Principal name

c-fos antibody

Alternative names for c-fos antibody

FOS, G0S7, Proto-oncogene protein c-fos, Cellular oncogene fos, G0/G1 switch regulatory protein 7

SwissProt ID

O02761 (Sheep), O77628 (Bovin), O97930 (Pig), P01100 (Human), P01101 (Mouse), P11939 (Chick), P12841 (Rat), Q8HZP6 (Felca), Q9Z2Q8 (Crigr)

Gene ID

2353 (FOS), 314322 (Fos)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Bov (Bovine), Chk (Chicken), Por (Porcine), Fe (Feline), Hst (Hamster), Rb (Rabbit), Sh (Sheep), Mky (Monkey)

Available hosts

Rabbit, Mouse, Chicken, Sheep, Goat

Available applications

Frozen Sections (C), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E), Paraffin Sections (P), Dot blot (Dot), Flow Cytometry (F), Electrophoretic Mobility Shift Assay (EMSA)

Background of c-fos antibody

The Fos family of nuclear oncogenes includes c-Fos, FosB, Fos-related antigen 1 (FRA1), and Fos-related antigen 2 (FRA2) (1). While most Fos proteins exist as a single isoform, the FosB protein exists as two isoforms: full-length FosB and a shorter form, FosB2 (Delta FosB), that lacks the carboxy-termil 101 amino acids (1-3). The expression of Fos proteins is rapidly and transiently induced by a variety of extracellular stimuli including growth factors, cytokines, neurotransmitters, polypeptide hormones, and stress. Fos proteins dimerize with Jun proteins (c-Jun, JunB, and JunD) to form Activator Protein-1 (AP-1), a transcription factor that binds to TRE/AP-1 elements and activates transcription. Fos and Jun proteins contain the leucine-zipper motif that mediates dimerization and an adjacent basic domain that binds to D. The various Fos/Jun heterodimers differ in their ability to transactivate AP-1 dependent genes. In addition to increased expression, phosphorylation of Fos proteins by Erk kises in response to extracellular stimuli may further increase transcriptiol activity (4-6). Phosphorylation of c-Fos at Ser32 and Thr232 by Erk5 increases protein stability and nuclear localization (5). Phosphorylation of FRA1 at Ser252 and Ser265 by Erk1/2 increases protein stability and leads to overexpression of FRA1 in cancer cells (6). Following growth factor stimulation, expression of FosB and c-Fos in quiescent fibroblasts is immediate, but very short-lived, with protein levels dissipating after several hours (7). FRA1 and FRA2 expression persists longer and appreciable levels can be detected in asynchronously growing cells (8). Deregulated expression of c-Fos, FosB, or FRA2 can result in neoplastic cellular transformation; however, Delta FosB lacks the ability to transform cells (2,3).

General readings

General / background references:Shaulian E & Karin M AP-1 in cell proliferation and survival. Oncogene 20:2390-400 (2001). PubMed PMID: 11402335
Liebermann DA et al. AP-1 (Fos/Jun) transcription factors in hematopoietic differentiation and apoptosis. Int J Oncol 12:685-700 (1998). PubMed PMID: 9472112
Karin M et al. AP-1 function and regulation. Curr Opin Cell Biol 9:240-6 (1997). PubMed PMID: 9069263
Curran T Fos and Jun: oncogenic transcription factors. Tohoku J Exp Med 168:169-74 (1992). PubMed PMID: 1306301
Bos TJ et al. The carboxy terminus of the viral Jun oncoprotein is required for complex formation with the cellular Fos protein. Oncogene 4:123-6 (1989). PubMed PMID: 2494630
Rauscher FJ et al. Fos and Jun bind cooperatively to the AP-1 site: reconstitution in vitro. Genes Dev 2:1687-99 (1988). PubMed PMID: 2467839

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

c-fos antibody

Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-FOS Rabbit Polyclonal antibody. (TA590201) Rabbit IgG Purified Can, Hu, Mky, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

c-fos antibody

Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-FOS mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806833) Mouse IgG1 OTI2D2 Purified Hu P
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

c-fos antibody

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-FOS mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806862) Mouse IgG1 OTI3B8 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

c-fos antibody

Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-FOS mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA806863)(1:150)(1:200) Mouse IgG1 OTI1E3 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

c-fos antibody

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-FOS mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806977) Mouse IgG1 OTI7D6 Purified Hu P
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

c-fos antibody

Immunohistochemical analysis of paraffin-embedded colon cancer tissues (left) and  cerebellum tissues (right) using FOS antibody Cat.-No AM06762SU-N with DAB staining. Mouse IgG1 2G2 Ascites Hu E, F, P, WB
0.1 ml / €430.00
  Acris Antibodies GmbH

c-fos antibody

Immunohistochemistry-Paraffin: c-Fos Antibody [NB110-75039] - IHC on rat spinal cord (paraffin section) using Rabbit antibody to cFos (c-fos): whole serum at 1: 300 dilution incubated overnight at 4C and developed with ABC/DAB/Ni. Rabbit Serum Ms, Rt C, ICC/IF, P, WB
0.1 ml / €400.00
  Novus Biologicals Inc.

c-fos (N-term) antibody

Representative picture of c-FOS expression in HEK293 cells, visualized with clonal rabbit anti-c-FOS monospecific antibody. Primary antibody dilution - 1:500 (See Immunocytochemistry (ICC) protocol) Rabbit IgG F14-R Aff - Purified Hu, Ms, Rt C, E, ICC/IF, IP, P, WB
0.1 ml / €550.00
  DB Biotech, spol. s r.o.

Sample sizes available

c-fos (N-term) antibody

Representative picture of c-FOS expression in HEK293 cells, visualized with clonal rabbit anti-c-FOS monospecific antibody. Primary antibody dilution - 1:500 (See Immunocytochemistry (ICC) protocol) Rabbit IgG F14-R Aff - Purified Hu, Ms, Rt C, E, ICC/IF, IP, P, WB
50 µl / €370.00
  DB Biotech, spol. s r.o.

Sample sizes available

c-fos (C-term) antibody

Anti-c-FOS (DB 186)Western blot of c-FOS: lane 1 – 100 ng; lane 2 – 200 ng; lane 3 – 500 ng of recombinant human c-FOS (N-term GST c-FOS, aa 1 – 381; Novus Biologicals, H00002353-P01). Rabbit IgG A16-L Aff - Purified Hu, Ms, Rt C, E, ICC/IF, IP, P, WB
0.1 ml / €550.00
  DB Biotech, spol. s r.o.

Sample sizes available

c-fos (C-term) antibody

Anti-c-FOS (DB 186)Western blot of c-FOS: lane 1 – 100 ng; lane 2 – 200 ng; lane 3 – 500 ng of recombinant human c-FOS (N-term GST c-FOS, aa 1 – 381; Novus Biologicals, H00002353-P01). Rabbit IgG A16-L Aff - Purified Hu, Ms, Rt C, E, ICC/IF, IP, P, WB
50 µl / €370.00
  DB Biotech, spol. s r.o.

Sample sizes available

c-fos antibody

Human skin stained with Anti-c-fos Rabbit Purified Hu P
0.5 ml / €360.00
  Acris Antibodies GmbH

c-fos antibody

Human skin stained with Anti-c-fos Rabbit Purified Hu P
1 ml / €490.00
  Acris Antibodies GmbH

c-fos antibody

Human skin stained with Anti-c-fos Rabbit Purified Hu P
0.1 ml / €190.00
  Acris Antibodies GmbH

c-fos antibody

(0.3ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Purified Bov, Can, Fe, Hu, Por WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

c-fos antibody

Western blot analysis of extracts from RAW264.7 cells, using FOS (Ab-232) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

c-fos antibody

Western blot analysis of extracts from HepG2 cells, using Fos antibody (#TA312213).The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

c-fos antibody

Western blot analysis on K562 cell lysate using Fos Antibody Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

c-fos antibody

c-fos Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

c-fos antibody

Western blot analysis of extracts of mouse intestine, using c-Fos antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

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