c-fos antibody

Principal name

c-fos antibody

Alternative names for c-fos antibody

FOS, G0S7, Proto-oncogene protein c-fos, Cellular oncogene fos, G0/G1 switch regulatory protein 7

SwissProt ID

O02761 (Sheep), O77628 (Bovin), O97930 (Pig), P01100 (Human), P01101 (Mouse), P11939 (Chick), P12841 (Rat), Q8HZP6 (Felca), Q9Z2Q8 (Crigr)

Gene ID

2353 (FOS), 14281 (Fos)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Mky (Monkey), Bov (Bovine), Fe (Feline), Por (Porcine)

Available hosts

Rabbit, Mouse, Goat

Available applications

Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Flow Cytometry (F), Immunoprecipitation (IP), Electrophoretic Mobility Shift Assay (EMSA)

Background of c-fos antibody

The Fos family of nuclear oncogenes includes c-Fos, FosB, Fos-related antigen 1 (FRA1), and Fos-related antigen 2 (FRA2) (1). While most Fos proteins exist as a single isoform, the FosB protein exists as two isoforms: full-length FosB and a shorter form, FosB2 (Delta FosB), that lacks the carboxy-termil 101 amino acids (1-3). The expression of Fos proteins is rapidly and transiently induced by a variety of extracellular stimuli including growth factors, cytokines, neurotransmitters, polypeptide hormones, and stress. Fos proteins dimerize with Jun proteins (c-Jun, JunB, and JunD) to form Activator Protein-1 (AP-1), a transcription factor that binds to TRE/AP-1 elements and activates transcription. Fos and Jun proteins contain the leucine-zipper motif that mediates dimerization and an adjacent basic domain that binds to D. The various Fos/Jun heterodimers differ in their ability to transactivate AP-1 dependent genes. In addition to increased expression, phosphorylation of Fos proteins by Erk kises in response to extracellular stimuli may further increase transcriptiol activity (4-6). Phosphorylation of c-Fos at Ser32 and Thr232 by Erk5 increases protein stability and nuclear localization (5). Phosphorylation of FRA1 at Ser252 and Ser265 by Erk1/2 increases protein stability and leads to overexpression of FRA1 in cancer cells (6). Following growth factor stimulation, expression of FosB and c-Fos in quiescent fibroblasts is immediate, but very short-lived, with protein levels dissipating after several hours (7). FRA1 and FRA2 expression persists longer and appreciable levels can be detected in asynchronously growing cells (8). Deregulated expression of c-Fos, FosB, or FRA2 can result in neoplastic cellular transformation; however, Delta FosB lacks the ability to transform cells (2,3).

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

c-fos antibody

  Rabbit Polyclonal c-Fos Antibody  
Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-FOS Rabbit Polyclonal antibody. (TA590201) Rabbit IgG Purified Can, Hu, Mky, Ms, Rt E, P, WB
0.1 mg / €409.00
  OriGene Technologies, Inc.

c-fos (N-term) antibody

Representative picture of c-FOS expression in HEK293 cells, visualized with clonal rabbit anti-c-FOS monospecific antibody. Primary antibody dilution - 1:500 (See Immunocytochemistry (ICC) protocol) Rabbit IgG F14-R Aff - Purified Hu, Ms, Rt C, E, ICC/IF, IP, P, WB
0.1 ml / €550.00
  DB Biotech, spol. s r.o.

c-fos (N-term) antibody

Representative picture of c-FOS expression in HEK293 cells, visualized with clonal rabbit anti-c-FOS monospecific antibody. Primary antibody dilution - 1:500 (See Immunocytochemistry (ICC) protocol) Rabbit IgG F14-R Aff - Purified Hu, Ms, Rt C, E, ICC/IF, IP, P, WB
50 µl / €370.00
  DB Biotech, spol. s r.o.

c-fos (C-term) antibody

Anti-c-FOS (DB 186)Western blot of c-FOS: lane 1 – 100 ng; lane 2 – 200 ng; lane 3 – 500 ng of recombinant human c-FOS (N-term GST c-FOS, aa 1 – 381; Novus Biologicals, H00002353-P01). Rabbit IgG A16-L Aff - Purified Hu, Ms, Rt C, E, ICC/IF, IP, P, WB
0.1 ml / €550.00
  DB Biotech, spol. s r.o.

c-fos (C-term) antibody

Anti-c-FOS (DB 186)Western blot of c-FOS: lane 1 – 100 ng; lane 2 – 200 ng; lane 3 – 500 ng of recombinant human c-FOS (N-term GST c-FOS, aa 1 – 381; Novus Biologicals, H00002353-P01). Rabbit IgG A16-L Aff - Purified Hu, Ms, Rt C, E, ICC/IF, IP, P, WB
50 µl / €370.00
  DB Biotech, spol. s r.o.

c-fos antibody

  Rabbit polyclonal FOS (Ab-232) antibody  
Western blot analysis of extracts from RAW264.7 cells, using FOS (Ab-232) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms WB
0.1 ml / €335.00
  OriGene Technologies, Inc.

c-fos antibody

  Rabbit polyclonal anti-Fos antibody  
Western blot analysis of extracts from HepG2 cells, using Fos antibody (#TA312213).The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.

c-fos antibody

  Rabbit Polyclonal Fos Antibody  
Western blot analysis on K562 cell lysate using Fos Antibody Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €335.00
  OriGene Technologies, Inc.

c-fos antibody

  Rabbit Polyclonal Fos (Ser362) Antibody (Phospho-specific)  
Western blot analysis of Fos phosphorylation expression in forskolin treated K562 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €335.00
  OriGene Technologies, Inc.

c-fos antibody

  Rabbit Polyclonal Fos Antibody  
c-fos Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €335.00
  OriGene Technologies, Inc.

c-fos antibody

  Rabbit anti-FOS Polyclonal Antibody  
Western blot analysis of extracts of mouse intestine, using c-Fos antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €335.00
  OriGene Technologies, Inc.

c-fos antibody

  Rabbit Polyclonal Anti-FOS Antibody  
Gel: 10%SDS-PAGE, Lysate: 40 ug, Lane: Hela cells, Primary antibody: (FOS Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 15 seconds Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.

c-fos (N-term) (incl. pos. control) antibody

Detection of endogenous FOS: Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab FOS-8B5 (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec).
Lane 1: A431 
Lane 2: A549
Lane 3: SKOV3 
Lane 4: OVCAR5 
Lane 5: HaCaT 
Lane 6: PC3
Lane 7: HeLa
Lane 8: HepG2 Mouse IgG1 8B5 Purified Can, Hu, Ms, Rt C, E, WB
0.1 mg / €430.00
  OriGene Technologies GmbH

c-fos antibody

Immunohistochemistry (IHC) analyzes of Fos antibody (Cat.-No.: AP06125PU-N) in paraffin-embedded human breast carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  OriGene Technologies GmbH

c-fos antibody

Immunohistochemistry (IHC) analyzes of c-Fos antibody (Cat.-No.: AP21134PU-N) in paraffin-embedded human colon carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  OriGene Technologies GmbH

c-fos (N-term) antibody

Western blot with C-FOS Polyclonal Antibody Rabbit IgG Aff - Purified Hu, Ms, Rt WB
0.1 mg / €390.00
  OriGene Technologies GmbH

c-fos pThr232 antibody

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using FOS (Phospho-Thr232) antibody AP55783PU-N (left)or the same antibody preincubated with blocking peptide (right). Rabbit Aff - Purified Hu, Ms P, WB
0.1 mg / €340.00
  OriGene Technologies GmbH

c-fos pThr232 antibody

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using FOS (Phospho-Thr232) antibody AP55783PU-N (left)or the same antibody preincubated with blocking peptide (right). Rabbit Aff - Purified Hu, Ms P, WB
50 µg / €270.00
  OriGene Technologies GmbH

c-fos pSer362 antibody

Immunohistochemistry analysis of paraffin-embedded human thyroid gland, using Fos (Phospho-Ser362) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
50 µg / €230.00
  Assay Biotech

c-fos pSer362 antibody

Immunohistochemistry analysis of paraffin-embedded human thyroid gland, using Fos (Phospho-Ser362) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €330.00
  Assay Biotech

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