c-Myc antibody

Principal name

c-Myc antibody

Alternative names for c-Myc antibody

Transcription factor p64, BHLHE39, Myc proto-oncogene protein, Class E basic helix-loop-helix protein 39

SwissProt ID

P01106 (Human), P01108 (Mouse), P01109 (Chick), P09416 (Rat), Q28350 (Canfa), Q28566 (Sheep), Q29031 (Pig), Q2HJ27 (Bovin)

Gene ID

4609 (MYC), 17869 (Myc), 24577

Available reactivities

Hu (Human), Can (Canine), Ms (Mouse), Por (Porcine), Rb (Rabbit), Rt (Rat), Av (Birds), Mam (Mammalia), Chimp (Chimpanzee), Gorilla, Bov (Bovine), Chk (Chicken), Sh (Sheep), Ze (Zebrafish), Mky (Monkey), Ye (Yeast), Dros (Drosophila)

Available hosts

Rabbit, Rat, Mouse, Chicken, Goat

Available applications

Frozen Sections (C), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Flow Cytometry (F), Paraffin Sections (P), Dot blot (Dot), ELISA (detection) (E(detection)), Luminex (LMNX), ChIP assay (ChIP)

Background of c-Myc antibody

MYC is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this MYC gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma.The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt\'s lymphomas, suggesting its importance in the normal function of this gene.The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt\'s lymphomas, suggesting its importance in the normal function of this gene.

General readings

IMMUNOPRECIPITATION PROCEDURE
The work can be performed in 1.5 ml micro-centrifuge tubes or in spin columns.
1. Thoroughly resuspend the Anti-Myc Agarose by inverting the tube or vial several times.
2. Add 20-50 µl 50% slurry of Anti-Myc Agarose into cell lysate using a widebore pipette tip.
The lysate should be fresh, and for a well expressed tagged protein, 200 µl lysate (200-500 ug total protein) usually yields a good IP result.
3. Incubate with gentle mixing for 2 h to overnight at 4°C.
4. Wash the beads with 1 ml TBS buffer or lysis buffer, such as RIPA (50 mM Tris HCl, pH 7.4, 150 mM NaCl, 1 mM EDTA, 1% NP-40, 0.5% sodium deoxycholate), centrifuge for 3 min at 2,000× g, and discard the supernatant. Wash 3 times, avoid losing beads during washes.
5. Elution of the Myc tagged protein.
Option 1. Elution with elution buffer.
Add 30-50 µl elution buffer to the beads, gently tap the tube to mix well, centrifuge for 3 min, transfer the supernatant very carefully to a fresh tube (Avoid transferring any beads).
Note: Neutralize the eluant immediately by add 1 ul of 1.5 M Tris, pH 9.0 per 20 µl Elution buffer.
Option 2. Elution with Myc peptide
Add 30-50 µl Myc peptide solution (100 ug/ml Myc peptide in TBS buffer), gently tap the tube to mix well, incubate for 10 min, centrifuge for 3 min, and transfer the supernant to a fresh tube. TBS buffer: 50 mM Tris HCl, 150 mM NaCl, pH 7.4.
Option 3. Elution with SDS loading buffer
Add 30 µl 2x SDS loading buffer, gently tap the tube to mix well, boil at 100 °C for 5 min, centrifuge for 3 min, transfer the supernatant to a fresh tube.
Note: in this case, the supernatant contains not only the binding proteins, but also IgG (heavy and light chains).
6. Prepare SDS-PAGE gel for western blotting or proceed to other assays.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

c-Myc antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-MYC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500002) Mouse IgG2b OTI1A6 Purified Hu F, ICC/IF, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

c-Myc antibody

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MYC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500003) Mouse IgG2a OTI3F2 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

c-Myc antibody

Western Blot: c-Myc Antibody [TA336686] - Analysis of c-Myc on Jurkat whole cell extract using TA336686. Rabbit Purified Hu, Ms C, E, ICC/IF, IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

c-Myc antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-MYC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500004) Mouse IgG2b OTI5G3 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

c-Myc antibody

Immunofluorescent staining of HeLa cells using anti-MYC mouse monoclonal antibody (TA507203). Mouse IgG2a OTI5E9G2 Purified Can, Hu, Mky, Rt ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

c-Myc antibody

Western blot analysis of MYC (arrow) using rabbit polyclonal MYC Antibody (T58) (Cat.#TA302042). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MYC gene (Lane 2) (Origene Technologies). Rabbit Ig Aff - Purified Hu, Ms, Ze P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

c-Myc antibody

Western blot analysis of MYC (arrow) using rabbit polyclonal MYC Antibody (S62) (Cat.#TA302044). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MYC gene (Lane 2) (Origene Technologies). Rabbit Ig Aff - Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

c-Myc antibody

Western blot analysis of MYC (arrow) using rabbit polyclonal MYC Antibody (S373) (Cat.#TA302045). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MYC gene (Lane 2) (Origene Technologies). Rabbit Ig Aff - Purified Hu, Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

c-Myc antibody

Sample (20 ug of whole cell lysate). A: human ESC. 10% SDS PAGE. TA307883 diluted at 1:1000. Rabbit IgG Aff - Purified Hu, Ms ChIP, F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

c-Myc antibody

Western blot analysis of MYC expression in Forskolin treated HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

c-Myc antibody

Western blot analysis of MYC expression in ovarycancer whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

c-Myc antibody

Western blot analysis of extracts from HuvEc cells, using MYC Antibody. The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

c-Myc antibody

c-Myc Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

c-Myc antibody

Western blot analysis of Myc phosphorylation expression in ovarycancer whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

c-Myc antibody

Western blot analysis of extracts from HUVEC cells, using Myc antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

c-Myc antibody

Jurkat  extract was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-c-myc monoclonal antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system. Intact (non-degraded) c-myc with a molecular weight of approximately 62 kD is detected in this Western blot experiment. Mouse IgG1 9E10 Purified Hu E, ICC/IF, IP, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

c-Myc antibody

Western Blot: c-Myc Antibody (9E11) [TA336584] - Analysis of c-Myc in Jurkat whole cell lysate. Mouse IgG2a 9E11 Purified Chk, Hu, Ms, Ye C, ChIP, E, F, IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

c-Myc antibody

Western Blot: c-Myc Antibody (9E10) [TA336685] - Analysis of c-myc in Jurkat cell lysates using TA336685. Mouse IgG1 9E10 Purified Dros, Hu, Ms C, E, F, ICC/IF, IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

c-Myc antibody

Western blot analysis on 293 cell lysate using MYC Antibody Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

c-Myc antibody

MYC ELISA with 3F2 Capture (TA600496) and 1A6 Detection (TA700496) Antibodies. Substrate used: Recombinant Human MYC (TP301611) Mouse IgG1 OTI3F2 Purified Hu E, LMNX
0.1 mg / €325.00
  OriGene Technologies, Inc.

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