C1q A subunit antibody

Principal name

C1q A subunit antibody

Alternative names for C1q A subunit antibody

Complement C1q, Complement 1q, Complement C1q subcomponent subunit A

SwissProt ID

P02745 (Human), P31720 (Rat), P98086 (Mouse), Q5E9E3 (Bovin), Q69DL0 (Pig)

Gene ID

712 (C1QA), 727 (C5)

Available reactivities

Ms (Mouse), Hu (Human), Rt (Rat), Bov (Bovine), Can (Canine), Eq (Equine), Por (Porcine), Rb (Rabbit)

Available hosts

Rat, Mouse, Sheep, Rabbit, Chicken, Goat

Available applications

Frozen Sections (C), Functional assay (FN), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Western blot / Immunoblot (WB), Immunodiffusion (ID), Flow Cytometry (F), ELISA (detection) (E(detection)), Radioimmunoassay (R), EPair for Elisa (EPair), Immunoprecipitation (IP)

Background of C1q A subunit antibody

The monoclonal antibody JL-1 recognizes the collagen-like region (CLR) of mouse C1q, a 459 kDa molecule consisting of three individual polypeptide chains. The antibody has been generated by immunization of C1q-/- C57BL/6 mice with purified mouse C1q. C1q forms together with C1r and C1s the C1 macromolecule, the first component of the classical complement pathway. Interaction of immune complexes with C1q induces a conformational change within the C1 complex, which results in activation of the classical pathway. C1q functions as recognition unit by binding to the heavy chain of IgG or IgM (Fc gamma and Fc micro) provided that the immunoglobulins are bound to their antigen. Furthermore, C1q can also recognize molecular patterns associated with pathogens and it can bind to apoptotic blebs, where it activates the classical complement pathway and mediates phagocytosis. As such, C1q promotes the clearance of apoptotic cells and subsequent exposure of auto antigens, thereby preventing stimulation of the immune system. C1q is predominantly produced by macrophages but also by follicular dendritic cells, interdigitating cells and cells of the monocyte-macrophage lineage. C1q deficiency has a profound effect on host defence and clearance of immune complexes. Absence of C1q may cause autoimmunity by impairment of the clearance of apoptotic cells. Inherited C1q deficiency is also associated with the development of systemic lupus erythematosus (SLE). The monoclonal antibody JL-1 is reactive with the collagen-like region (CLR) only, which is the same region to which autoantibodies in mice and humans are binding. Anti-C1q autoantibodies deposit in glomeruli together with C1q but induce overt renal disease only in the context of glomerular immune complex disease. This provides an explanation why anti-C1q antibodies are especially pathogenic in patients with SLE.

General readings

General / background references:Wasiliu M et al. Monoclonal antibodies to complement components without the need of their prior purification. I. Antibodies to mouse C1q. Hybridoma 8:615-22 (1989). PubMed PMID: 2482249

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

C1q antibody

C1q Mouse IgG1 3R9/2 Purified Hu C, E, F
0.1 ml / €400.00
  Acris Antibodies GmbH

C1q antibody

C1q Goat IgG FITC Hu C, E, ICC/IF, ID, IP
1 ml / €340.00
  Acris Antibodies GmbH

C1q antibody

C1q Goat Serum Hu ID, IP
1 ml / €250.00
  Acris Antibodies GmbH

C1q antibody

C1q Rabbit Serum Hu ID, IP
1 ml / €250.00
  Acris Antibodies GmbH

C1q antibody

C1q Goat IgG Aff - Purified Hu E
1 mg / €500.00
  Acris Antibodies GmbH

C1q antibody

C1q Mouse IgG1 242G3 Purified Hu E, FN, WB
50 µg / €290.00
  Acris Antibodies GmbH

C1q antibody

C1q Sheep FITC Hu C
1 ml / €400.00
  Acris Antibodies GmbH

C1q antibody

C1q Sheep IgG HRP Hu E
1 ml / €380.00
  Acris Antibodies GmbH

C1q antibody

C1q Sheep Purified Hu ID
1 ml / €400.00
  Acris Antibodies GmbH

C1q antibody

C1q Sheep IgG FITC Hu C, P
0.5 ml / €780.00
  Novus Biologicals Inc.

C1q antibody

Immunofluorescence: C1QA  Antibody (3R9/2) [NB100-64729] - C4d deposition in graft biopsies of patients with DGF. C4d (green, E) co-localized with C1QA (red, G) at peri-tubular capillary sites (merge, I). TO-PRO-3 (blue) was used to stain nuclei. Original magnifications were (E–J) ×630. Mouse IgG1 3R9/2 Purified Hu C, E, F
50 µl / €380.00
  Novus Biologicals Inc.

C1q antibody

C1q Rabbit IgG FITC Hu ICC/IF
0.5 ml / €780.00
  Novus Biologicals Inc.

C1q antibody

C1q Rat IgG1 7H8 Purified Ms C, E, FN, ICC/IF, P
50 µg / €400.00
  Novus Biologicals Inc.

C1q antibody

ELISA: C1QA  Antibody [NB300-345] - Human plasma C1q protein as test antigen. Affi-pure IgY as primary antibody (1.0 ug/ml), and Goat anti-IgY-HRP as 2nd antibody. ( anti-C1QA ) Chicken Aff - Purified Hu E, ICC/IF, WB
50 µg / €520.00
  Novus Biologicals Inc.

C1q A subunit antibody

WB Suggested Anti-C1QA Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Hela cell lysate Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

C1q A subunit antibody

C1q A subunit Rat IgG1 7H8 Purified Ms C, E, FN, IP
0.1 mg / €410.00
  Acris Antibodies GmbH

C1q A subunit antibody

C1q A subunit Mouse IgG2b JL-1 Biotin Hu, Ms, Rt C, E, FN, ICC/IF, WB
50 µg / €330.00
  Acris Antibodies GmbH

C1q A subunit antibody

C1q A subunit Mouse IgG2b JL-1 FITC Hu, Ms, Rt C, E, FN, ICC/IF, WB
0.1 mg / €490.00
  Acris Antibodies GmbH

C1q A subunit antibody

C1q A subunit Mouse IgG2b JL-1 Purified Hu, Ms, Rt C, E, FN, ICC/IF, WB
0.1 mg / €410.00
  Acris Antibodies GmbH

C1q A subunit antibody

C1QA MaxPab polyclonal antibody. Western Blot analysis of C1QA expression in human lung cancer. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

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