CACYBP antibody

Principal name

CACYBP antibody

Alternative names for CACYBP antibody

Calcyclin-binding protein, S100A6BP, SIP, PNAS-107, Siah-interacting protein, S100A6-binding protein

SwissProt ID

Q3T168 (Bovin), Q6AYK6 (Rat), Q9CXW3 (Mouse), Q9HB71 (Human)

Gene ID

27101 (CACYBP)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Chk (Chicken), African clawed frog, Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Paraffin Sections (P), ELISA (detection) (E(detection)), Frozen Sections (C)

Background of CACYBP antibody

CACYBP(Calcyclin-binding protein), also called SIP, is a protein that in humans is encoded by the CACYBP gene. The full-length SIP cDNA encodes a predicted 228-amino acid protein. Sequence analysis of the shortest cDNA derived by 2-hybrid screening revealed an 8-amino acid difference in the deduced open reading frame followed by a stop codon, resulting in a predicted 80-amino acid protein, SIP-short (SIPS). The CACYBP gene is mapped on 1q25.1. It may be involved in calcium-dependent ubiquitination and subsequent proteosomal degradation of target proteins. It probably serves as a molecular bridge in ubiquitin E3 complexes and participates in the ubiquitin-mediated degradation of beta-catenin. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. The C-terminal region of SIP that is homologous to SGT1 was able to complement defects in yeast strains containing SGT1 mutant alleles, demonstrating conservation of SGT1 and SIP protein function.

General readings

1. Matsuzawa, S., Reed, J. C. Siah-1, SIP, and Ebi collaborate in a novel pathway for beta-catenin
degradation linked to p53 responses.Molec. Cell 7: 915-926, 2001.
2. Sun S, Ning X, Liu J, et al. (2007). "Overexpressed CacyBP/SIP leads to the suppression of growth
in renal cell carcinoma.". Biochem. Biophys. Res. Commun. 356 (4): 864–71

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CACYBP antibody

Immunofluorescence of purified MaxPab antibody to CACYBP on HeLa cell. [antibody concentration 10 ug/ml] Mouse Purified Hu E(detection), ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

CACYBP antibody

Sample (50 ug of whole cell lysate). A: mouse brain. 12% SDS PAGE. TA308595 diluted at 1:1000. Rabbit IgG Aff - Purified Hu, Ms ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CACYBP antibody

WB Suggested Anti-CACYBP Antibody Titration: 5.0 ug/ml; Positive Control: HepG2 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CACYBP antibody

Immunohistochemical analysis of paraffin-embedded human brain glioma tissue showing nuclear localization using calcyclin mouse mAb with DAB staining. Mouse IgG1 7D11 Purified Hu E, P, WB
0.1 ml / €360.00
  Acris Antibodies GmbH

CACYBP antibody

The cell lysates of HeLa, 293T, Jurkat and A549(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CACYBP antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG2a AT3G8 Purified Hu E, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

CACYBP antibody

The cell lysates of HeLa, 293T, Jurkat and A549(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CACYBP antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG2a AT3G8 Purified Hu E, WB
50 µl / €220.00
  Acris Antibodies GmbH

CACYBP antibody

Western Blot analysis of CACYBP expression in transfected 293T cell line (H00027101-T01) by CACYBP MaxPab polyclonal antibody.

Lane 1: CACYBP transfected lysate(26.20 KDa).
Lane 2: Non-transfected lysate. Rabbit Serum Hu E(detection), IP, WB
0.1 ml / €380.00
  Abnova Taiwan Corp.

CACYBP antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between CACYBP and TBL1X HeLa cells were stained with anti-CACYBP rabbit purified polyclonal 1:1200 and anti-TBL1X mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

CACYBP antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between SKP1 and CACYBP. HeLa cells were stained with anti-SKP1 rabbit purified polyclonal 1:1200 and anti-CACYBP mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Mouse IgG1 2E3 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

CACYBP antibody

CACYBP Mouse IgG1 2E3 Hu E, WB
0.2 ml / €370.00
  Abnova Taiwan Corp.

CACYBP antibody

CACYBP Mouse IgG1 2E3 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

CACYBP antibody

Human HepG2; WB Suggested Anti-CACYBP Antibody Titration: 5.0ug/ml. Positive Control: HepG2 cell lysate; CACYBP antibody - middle region (ARP51259_T100) in Human HepG2 cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Rt WB
0.1 mg / €310.00
  AVIVA Systems Biology

CACYBP antibody

CACYBP Rabbit IgG Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €290.00
  Boster Immunoleader

CACYBP antibody

Western Blot: CACYBP Antibody [NBP1-49921] - Detection of Human CacyBP by Western Blot and Immunoprecipitation.  Samples:  Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells.  Antibodies: Affinity purified rabbit anti-CacyBP antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate.  CacyBP was also immunoprecipitated by rabbit anti-CacyBP antibody, which recognizes a downstream epitope.  For blotting immunoprecipitated CacyBP was used.   Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B). Rabbit Aff - Purified Hu WB
0.1 ml / €440.00
  Novus Biologicals Inc.

CACYBP antibody

Western Blot: CACYBP Antibody [NBP1-49922] - Detection of Human and Mouse CacyBP by Western Blot (h&m) and Immunoprecipitation (h).  Samples:  Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells.  Antibodies: Affinity purified rabbit anti-CacyBP antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate.  CacyBP was also immunoprecipitated by rabbit anti-CacyBP antibody, which recognizes an upstream epitope.  For blotting immunoprecipitated CacyBP was used.   Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B). Rabbit Aff - Purified Hu, Ms IP, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

CACYBP antibody

Immunohistochemistry-Paraffin: CACYBP Antibody [NBP1-87104] - staining of human urinary bladder shows strong cytoplasmic positivity in urothelial cells. Rabbit IgG Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

CACYBP (transcript variant 1)

CACYBP Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

CACYBP (transcript variant 2)

CACYBP Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

CACYBP (1-185, His-tag)

Recombinant human CACYBP, His-tagged: 15% SDS-PAGE (3 ug) Human Purified > 85 % by SDS – PAGE E. coli
0.5 mg / €670.00
  Acris Antibodies GmbH

CACYBP (1-185, His-tag)

Recombinant human CACYBP, His-tagged: 15% SDS-PAGE (3 ug) Human Purified > 85 % by SDS – PAGE E. coli
0.1 mg / €250.00
  Acris Antibodies GmbH

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