CAF-1 subunit A antibody

Principal name

CAF-1 subunit A antibody

Alternative names for CAF-1 subunit A antibody

CHAF1A, CAF, CAF1P150, CAF-I 150 kDa subunit, Chromatin assembly factor 1 subunit A, Chromatin assembly factor I p150 subunit

SwissProt ID

Q13111 (Human), Q9QWF0 (Mouse)

Gene ID

10036 (CHAF1A)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), PLA/Proximity ligation assay (PLA)

Background of CAF-1 subunit A antibody

Chromatin assembly factor-1 (CAF-1) is a multi protein complex that functions to assemble nucleosomes in a replication-dependent fashion. The CAF-1 complex is composed of three subunits, p150, p60 and p48 in stoichiometric amounts. The composition of CAF-1 is conserved among human Drosophila and yeast. Human CAF-1 deposits newly synthesized, acetylated histones onto replicated DNA where it localizes in S-phase cells. Localization of the CAF-1 complex to the newly synthesized DNA is mediated in part by PCNA which functions to "mark" the appropriate places for loading of CAF-1 and in fact, PCNA may be co-precipitated with CAF-1. During G1, G2, and S-phases, p150/p60 is found in a complex with a subfraction of p48 in the nucleus. During mitosis there is a hyperphos-phorylation of the p60 subunit and a corresponding enrichment in the cytosolic fraction of the p150 and p60 proteins. While characteristically a nuclear protein there is variation in level of staining intensity between cells and with the use of different fixatives. Although no disease states have been definitively identified as having alterations in expression of any of the CAF-1 subunits, the p150 subunit has been shown to map to 21q22.2, a region strongly associated with the major feature of Downs Syndrome.

General readings

1. Smith, S., et al. J. Biol. Chem., 266, 12041-12047 (1991)
2. Shibahara, K., et al. Proc. Natl. Acad. Sci. USA, 97, 7766-7771 (2000)
Clone p150-48 is used in these references.

13 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CAF-1 subunit A antibody

Western Blot: CAF-1 p150 Antibody (SS 1 1-13) - Western blot analysis of CAF1 p150 expression in Ntera2 whole cell lysate using anti-CAF1 p150. Mouse IgG1 SS 1 1-13 Purified Hu ICC/IF, IP, WB
0.2 ml / €325.00
  OriGene Technologies, Inc.

CAF-1 subunit A antibody

Western blot analysis of extracts from K562 cells and A549 cells, using CAF1A antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CAF-1 subunit A antibody

CAF-1 subunit A Mouse IgG1 1SS-48 Purified Hu ICC/IF, IP, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

CAF-1 subunit A antibody

Immunohistochemical detection of CAF-1 p150 on Paraffin Embedded Section of a Human tonsil with CAF1 P150 antibody AM20175AF-N. Mouse IgG1 p150-48 Azide Free Hu ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

CAF-1 subunit A antibody

CHAF1A monoclonal antibody (M02), clone 1C2 Western Blot analysis of CHAF1A expression in Hela NE ( Cat # L013V3 ). Mouse IgG2b 1C2 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

CAF-1 subunit A antibody

Immunofluorescent staining of CHAF1A on HeLa cells using CHAF1A monoclonal antibody, clone SS 1 1-13 ( Cat #MAB2356 ) ( Shibahara et al 1999 ). Mouse IgG1 SS 1 1-13 Hu ICC/IF, IP, WB
0.2 ml / €470.00
  Abnova Taiwan Corp.

CAF-1 subunit A antibody

WB and IP: Caf1p150 Antibody NB100-74608-Detection of Human Caf1p150 on HeLa whole cell lysate using NB100-74608. Caf1p150 was also immunoprecipitated by rabbit anti-Caf1p150 antibodies NB100-74609 and NB100-74610. Rabbit IgG Aff - Purified Hu IP, P, PLA, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

CAF-1 subunit A antibody

WB and IP: Caf1p150 Antibody [NB100-74609] - Detection of Human Caf1p150 on HeLa whole cell lysate using NB100-74609. Caf1p150 was also  immunoprecipitated by rabbit anti-Caf1p150 antibodies NB100-74608 and NB100-74610. Rabbit IgG Aff - Purified Hu IP, P, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

CAF-1 subunit A antibody

CAF-1 subunit A Mouse IgG1 SS 1 1-13 Ascites Hu ICC/IF, IP, WB
50 µl / €160.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

CAF-1 subunit A

CAF-1 subunit A Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

CAF-1 subunit A

CAF-1 subunit A Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

CAF-1 subunit A

CAF-1 subunit A Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

CHAF1A overexpression lysate

CHAF1A overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

13 Item(s)

per page
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