CAF-1 subunit B antibody

Principal name

CAF-1 subunit B antibody

Alternative names for CAF-1 subunit B antibody

CHAF1B, CAF1A, CAF1P60, MPHOSPH7, MPP7, Chromatin assembly factor 1 subunit B, Chromatin assembly factor I p60 subunit, M-phase phosphoprotein 7

SwissProt ID

Q13112 (Human), Q9D0N7 (Mouse)

Gene ID

8208 (CHAF1B), 110749 (Chaf1b)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Chk (Chicken), Ze (Zebrafish), African clawed frog, Mky (Monkey), Prim (Primate), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), ELISA (detection) (E(detection)), Flow Cytometry (F)

Background of CAF-1 subunit B antibody

Chromatin assembly factor I (CAF-I) is required for the assembly of histone octamers onto newly-replicated DNA. CAF-I is composed of three protein subunits, p50, p60, and p150. CHAF1B corresponds to the p60 subunit and is required for chromatin assembly after replication. CHAF1B is differentially phosphorylated in a cell cycle-dependent manner. In addition, it is normally found in the nucleus except during mitosis, when it is released into the cytoplasm. This protein is a member of the WD-repeat HIR1 family and may also be involved in DNA repair.Chromatin assembly factor I (CAF-I) is required for the assembly of histone octamers onto newly-replicated DNA. CAF-I is composed of three protein subunits, p50, p60, and p150. The protein encoded by this gene corresponds to the p60 subunit and is required for chromatin assembly after replication. The encoded protein is differentially phosphorylated in a cell cycle-dependent manner. In addition, it is normally found in the nucleus except during mitosis, when it is released into the cytoplasm. This protein is a member of the WD-repeat HIR1 family and may also be involved in DNA repair.

General readings

1. Iida, T., et al., Mol. Cell. Biol. 24, 217-227 (2004)
2. Iida, T., et al., Genes Cells 7, 997-1007 (2002)
3. Shibahara, K., et al. PNAS 97, 7766-7771 (2000)
4. Smith, S., et al., J. Biol. Chem. 266, 12041-12047 (1991)
Clone p60-24 is used in the reference 3 and 4.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CAF-1 subunit B antibody

CHAF1B antibody - N-terminal region validated by WB using Mouse brains at 1: 1000. Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

CAF-1 subunit B antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-CHAF1B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506840) Mouse IgG1 OTI4F7 Purified Can, Hu, Mky, Ms, Rt ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CAF-1 subunit B antibody

Human HepG2; Host:Rabbit. Target Name:CHAF1B. Sample Tissue:HepG2. Antibody Dilution: 1.0ug/ml. CHAF1B is supported by BioGPS gene expression data to be expressed in HepG2.; CHAF1B antibody - N-terminal region (ARP45826_P050) in Human HepG2 cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

CAF-1 subunit B antibody

Western Blot: CHAF1B Antibody (SS 53) TA301488 - A. RKO cells with CAF-1 p150, CAF-1 p60 and PCNA antibodies. B. p150 RNAi knockdown Mouse IgG1 SS 53 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CAF-1 subunit B antibody

Western blot analysis of extracts from Jurkat cells, using CAF1B antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CAF-1 subunit B antibody

Western Blot: CHAF1B Antibody (SS 24 1-68) [TA336654] - Analysis of CAF-1 p60 expression in 1) HeLa and 2) Cos7 whole cell lysates using TA336654. Mouse IgG1 SS 24 1-68 Purified Hu, Ms, Prim ICC/IF, IP, WB
50 µl / €325.00
  OriGene Technologies, Inc.

CAF-1 subunit B antibody

WB Suggested Anti-CHAF1B Antibody Titration: 5.0 ug/ml; Positive Control: Jurkat cell lysate.CHAF1B is supported by BioGPS gene expression data to be expressed in Jurkat Rabbit IgG Purified Hu P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CAF-1 subunit B antibody

Anti-CHAF1B mouse monoclonal antibody (TA506841) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CHAF1B(RC202858). Mouse IgG1 OTI2D5 Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CAF-1 subunit B antibody

CAF-1 subunit B Rabbit IgG I15-P FITC Hu F
100 Tests / €560.00
  DB Biotech, spol. s r.o.

Sample sizes available

CAF-1 subunit B antibody

Immunocytochemical detection of CAF-1 p60 on Acetone fixed HEp-II cells with CAF1P60 antibody AM20176AF-N. Mouse IgG1 p60-24 Azide Free Hu ICC/IF, IP, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

CAF-1 subunit B antibody

Western blot (WB) analysis of CAF-1 p60 antibody (Cat.-No.: AP01236PU-N) in extracts from Jurkat cells. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

CAF-1 subunit B antibody

CAF-1 subunit B Mouse Purified Hu, Rt E(detection), ICC/IF, P, WB
50 µg / €370.00
  Abnova Taiwan Corp.

CAF-1 subunit B antibody

CAF-1 subunit B Mouse IgG 2G7 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

CAF-1 subunit B antibody

Immunocytochemical staining of CHAF1B in HeLa cells using CHAF1B monoclonal antibody, clone SS 24 1-68 ( Cat #MAB2354 ) ( Smith and Stillman 1991 ). Mouse IgG1 SS 24 1-68 Hu, Ms ICC/IF, IP
50 µl / €470.00
  Abnova Taiwan Corp.

CAF-1 subunit B antibody

Western Blot analysis of : 
A. RKO cells with p150 antibody and CHAF1B monoclonal antibody, clone SS 53 ( Cat #MAB2355 ).
B. p150 RNAi knockdown. Mouse IgG1 SS 53 Hu IP, WB
0.1 ml / €470.00
  Abnova Taiwan Corp.

CAF-1 subunit B antibody

Western blot analysis of extracts from Jurkat cells, using CAF1B Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €205.00
  Assay Biotech

CAF-1 subunit B antibody

Western blot analysis of extracts from Jurkat cells, using CAF1B Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €310.00
  Assay Biotech

CAF-1 subunit B antibody

Human kidney; CHAF1B antibody - C-terminal region (ARP45827_T100) in Human kidney cells using Immunohistochemistry Rabbit Aff - Purified Hu P, WB
0.1 mg / €310.00
  AVIVA Systems Biology

CRISPR

Catalog No. Species Donor Vector  

CHAF1B - human gene knockout kit via CRISPR

CHAF1B - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

CAF-1 subunit B

CAF-1 subunit B Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

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