CAF-1 subunit B antibody

Principal name

CAF-1 subunit B antibody

Alternative names for CAF-1 subunit B antibody

CHAF1B, CAF1A, CAF1P60, MPHOSPH7, MPP7, Chromatin assembly factor 1 subunit B, Chromatin assembly factor I p60 subunit, M-phase phosphoprotein 7

SwissProt ID

Q13112 (Human), Q9D0N7 (Mouse)

Gene ID

8208 (CHAF1B)

Available reactivities

Hu (Human), Mky (Monkey), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Chk (Chicken), Ze (Zebrafish), African clawed frog, GP (Guinea Pig), Por (Porcine), Rb (Rabbit), Prim (Primate), Eq (Equine)

Available hosts

Mouse, Rabbit

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Flow Cytometry (F), ELISA (detection) (E(detection)), Frozen Sections (C)

Background of CAF-1 subunit B antibody

Chromatin assembly factor-1 (CAF-1) is a multi protein complex that functions to assemble nucleosomes in a replication-dependent fashion. The CAF-1 complex is composed of three subunits, p150, p60 and p48 in stoichiometric amounts. The composition of CAF-1 is conserved among human Drosophila and yeast. Human CAF-1 deposits newly synthesized, acetylated histones onto replicated DNA where it localizes in S-phase cells. Localization of the CAF-1 complex to the newly synthesized DNA is mediated in part by PCNA which functions to \mark\" the appropriate places for loading of CAF-1 and in fact, PCNA may be co-precipitated with CAF-1. During G1, G2, and S-phases, p150/p60 is found in a complex with a subfraction of p48 in the nucleus. During mitosis there is a hyperphos-phorylation of the p60 subunit and a corresponding enrichment in the cytosolic fraction of the p150 and p60 proteins. While characteristically a nuclear protein there is variation in level of staining intensity between cells and with the use of different fixatives. Although no disease states have been definitively identified as having alterations in expression of any of the CAF-1 subunits, the p150 subunit has been shown to map to 21q22.2, a region strongly associated with the major feature of Downs Syndrome."""

General readings

1. Iida, T., et al., Mol. Cell. Biol. 24, 217-227 (2004)
2. Iida, T., et al., Genes Cells 7, 997-1007 (2002)
3. Shibahara, K., et al. PNAS 97, 7766-7771 (2000)
4. Smith, S., et al., J. Biol. Chem. 266, 12041-12047 (1991)
Clone p60-24 is used in the reference 3 and 4.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CAF-1 subunit B antibody

CHAF1B antibody - N-terminal region validated by WB using Mouse brains at 1: 1000. Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

CAF-1 subunit B antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-CHAF1B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506840) Mouse IgG1 OTI4F7 Purified Can, Hu, Mky, Ms, Rt ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CAF-1 subunit B antibody

Human HepG2; Host:Rabbit. Target Name:CHAF1B. Sample Tissue:HepG2. Antibody Dilution: 1.0ug/ml. CHAF1B is supported by BioGPS gene expression data to be expressed in HepG2.; CHAF1B antibody - N-terminal region (ARP45826_P050) in Human HepG2 cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

CAF-1 subunit B antibody

Western Blot: CHAF1B Antibody (SS 53) TA301488 - A. RKO cells with CAF-1 p150, CAF-1 p60 and PCNA antibodies. B. p150 RNAi knockdown Mouse IgG1 SS 53 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CAF-1 subunit B antibody

Western blot analysis of extracts from Jurkat cells, using CAF1B antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CAF-1 subunit B antibody

Western Blot: CHAF1B Antibody (SS 24 1-68) [TA336654] - Analysis of CAF-1 p60 expression in 1) HeLa and 2) Cos7 whole cell lysates using TA336654. Mouse IgG1 SS 24 1-68 Purified Hu, Ms, Prim ICC/IF, IP, WB
50 µl / €325.00
  OriGene Technologies, Inc.

CAF-1 subunit B antibody

WB Suggested Anti-CHAF1B Antibody Titration: 5.0 ug/ml; Positive Control: Jurkat cell lysate.CHAF1B is supported by BioGPS gene expression data to be expressed in Jurkat Rabbit IgG Purified Hu P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CAF-1 subunit B antibody

Anti-CHAF1B mouse monoclonal antibody (TA506841) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CHAF1B(RC202858). Mouse IgG1 OTI2D5 Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CAF-1 subunit B antibody

CAF-1 subunit B Rabbit IgG I15-P FITC Hu F
100 Tests / €560.00
  DB Biotech, spol. s r.o.

Sample sizes available

CAF-1 subunit B antibody

Immunocytochemical detection of CAF-1 p60 on Acetone fixed HEp-II cells with CAF1P60 antibody AM20176AF-N. Mouse IgG1 p60-24 Azide Free Hu ICC/IF, IP, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

CAF-1 subunit B antibody

Western blot (WB) analysis of CAF-1 p60 antibody (Cat.-No.: AP01236PU-N) in extracts from Jurkat cells. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

CAF-1 subunit B antibody

CAF-1 subunit B Mouse Purified Hu, Rt E(detection), ICC/IF, P, WB
50 µg / €370.00
  Abnova Taiwan Corp.

CAF-1 subunit B antibody

CAF-1 subunit B Mouse IgG 2G7 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

CAF-1 subunit B antibody

Immunocytochemical staining of CHAF1B in HeLa cells using CHAF1B monoclonal antibody, clone SS 24 1-68 ( Cat #MAB2354 ) ( Smith and Stillman 1991 ). Mouse IgG1 SS 24 1-68 Hu, Ms ICC/IF, IP
50 µl / €470.00
  Abnova Taiwan Corp.

CAF-1 subunit B antibody

Western Blot analysis of : 
A. RKO cells with p150 antibody and CHAF1B monoclonal antibody, clone SS 53 ( Cat #MAB2355 ).
B. p150 RNAi knockdown. Mouse IgG1 SS 53 Hu IP, WB
0.1 ml / €470.00
  Abnova Taiwan Corp.

CAF-1 subunit B antibody

Western blot analysis of extracts from Jurkat cells, using CAF1B Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €205.00
  Assay Biotech

CAF-1 subunit B antibody

Western blot analysis of extracts from Jurkat cells, using CAF1B Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €310.00
  Assay Biotech

CAF-1 subunit B antibody

Human kidney; CHAF1B antibody - C-terminal region (ARP45827_T100) in Human kidney cells using Immunohistochemistry Rabbit Aff - Purified Hu P, WB
0.1 mg / €310.00
  AVIVA Systems Biology

CAF-1 subunit B antibody

Immunohistochemistry-Paraffin: CAF-1 p60 Antibody [NB100-57523] - FFPE section of human seminoma.  Affinity purified rabbit anti-CAF-1 p60 used at a dilution of 1:250.  Detection: DAB staining using Immunohistochemistry Accessory Kit Rabbit Aff - Purified Hu IP, P, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

CAF-1 subunit B antibody

CAF-1 subunit B Mouse IgG1 SS 24 1-68 Ascites Hu, Mky, Ms ICC/IF, IP, WB
25 µl / €160.00
  Novus Biologicals Inc.

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