Calnexin antibody

Principal name

Calnexin antibody

Alternative names for Calnexin antibody

IP90, p90, CANX, ER Marker, Major histocompatibility complex class I antigen-binding protein p88

SwissProt ID

P24643 (Canfa), P27824 (Human), P35564 (Mouse), P35565 (Rat)

Gene ID

821 (CANX), 403908

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), GP (Guinea Pig), Hst (Hamster), Mky (Monkey), Por (Porcine), Quail, Rb (Rabbit), Rt (Rat), Sh (Sheep), Xen (Xenopus), Can (Canine), Chk (Chicken), Dros (Drosophila), Eq (Equine), Ze (Zebrafish), Gt (Goat), Ye (Yeast)

Available hosts

Mouse, Rabbit, Goat

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Flow Cytometry (F), Enzyme Immunoassay (E), Frozen Sections (C)

Background of Calnexin antibody

Calnexin, also referred to as IP90, p88 and p90, is an ~90 kDa integral membrane protein of the endoplasmic reticulum (ER). Many resident ER proteins act as molecular chaperones and participate in the proper folding of polypeptides and their assembly into multisubunit proteins. Studies indicate that calnexin associates with the major histocompatability complex (MHC) class I heavy chains, partial complexes of the T cell receptor and B cell membrane immunoglobulin, but not with completed receptor complexes. It has been shown that calnexin is a chaperone that retains incompletely or improperly folded proteins in the ER. The sequence Lys-Asp-Glu-Leu (KDEL) or a closely related sequence, is present at the carboxy-terminus of soluble ER resident proteins such as GRP 78 and GRP 94 and protein disulfide isomerase. Integral membrane ER resident proteins, like calnexin, often lack this KDEL sequence but contain positively charged cytosolic residues that ensure ER retention. Calnexin contains a large ER luminal domain (461 amino acids), a transmembrane segment (22 amino acids), and a cytoplasmic tail (89 amino acids). These features distinguish calnexin from soluble ER chaperones that cannot interact with the transmembrane and cytosolic domains of integral membrane proteins. The amino acid sequence of calnexin is highly conserved among species, and shares regions of high sequence homology with calreticulin.

General readings

1. Novgorodov SA  et al. Developmentally regulated ceramide synthase 6 increases mitochondrial Ca2+ loading capacity and promotes apoptosis. J Biol Chem 286:4644-58 (2011). WB ; Rat .
2. Novgorodov SA  et al. Novel Pathway of Ceramide Production in Mitochondria: THIOESTERASE AND NEUTRAL CERAMIDASE PRODUCE CERAMIDE FROM SPHINGOSINE AND ACYL-CoA. J Biol Chem 286:25352-62 (2011). WB ; Mouse, Rat .
3. Ramachandra L  et al. Mycobacterium tuberculosis synergizes with ATP to induce release of microvesicles and exosomes containing major histocompatibility complex class II molecules capable of antigen presentation. Infect Immun 78:5116-25 (2010). WB ; Mouse .

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Calnexin antibody

Western blot analysis of Calnexin-NT in HeLa cell lysates using a 1:1000 dilution of TA309294. Rabbit IgG Serum Bov, Can, Chk, GP, Hst, Hu, Mky, Ms, Por, Rb, Rt, Sh, Xen ICC/IF, IP, WB
50 µl / €325.00
  OriGene Technologies, Inc.

Calnexin antibody

Western blot analysis of Calnexin-NT in HeLa cell lysates using a 1:1000 dilution of TA309294. Rabbit IgG Serum Bov, Can, Chk, GP, Hst, Hu, Mky, Ms, Por, Rb, Rt, Sh, Xen ICC/IF, IP, WB
0.2 ml / €325.00
  OriGene Technologies, Inc.

Calnexin antibody

Western Blot: Calnexin Antibody [TA336278] - A dilution of 1/2000 used in TBS with 0.05% Tween-20. Bands visualized by ECL using goat anti-rabbit HRP at 1:2000. Rabbit Purified Bov, Chk, Hu, Ms, Rt C, ICC/IF, IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Calnexin antibody

Thyroid, Human: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG2b 3H4A7 Ascites Bear, Bov, Bt, Can, Chk, Elephant, Eq, Fi, Hst, Hu, L, Mky, Ms, Platypus, Por, Prim, Rb, Rt, Tr, Xen E, ICC/IF, P, WB
50 µl / €470.00
  Acris Antibodies GmbH

Calnexin antibody

TA305929 (0.3ug/ml) staining of Mouse Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Calnexin antibody

Western blot analysis of Calnexin-CT in rat tissues using a 1:1000 dilution of TA309295. Rabbit Purified Bov, Can, Chk, Dros, Hst, Hu, Mky, Ms, Por, Quail, Rb, Rt, Sh, Xen C, F, ICC/IF, IP, WB
50 µl / €325.00
  OriGene Technologies, Inc.

Calnexin antibody

Western blot analysis of purified calnexin-CT in a rat tissue mix using a 1:1000 dilution of TA309297. Rabbit Purified Bov, Can, Chk, Dros, Hst, Hu, Mky, Ms, Por, Quail, Rb, Rt, Sh, Xen C, F, ICC/IF, IP, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Calnexin antibody

Western blot analysis of purified calnexin-CT in a rat tissue mix using a 1:1000 dilution of the antibody Rabbit Purified Bov, Can, GP, Hst, Hu, Mky, Ms, Por, Quail, Rb, Rt, Sh, Xen WB
0.2 mg / €325.00
  OriGene Technologies, Inc.

Calnexin antibody

Western Blot: Calnexin Antibody [TA336279] - WB analysis of Calnexin in HeLa whole cell lysate. Rabbit Purified Hst, Hu, Ms, Rt C, ICC/IF, IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Calnexin antibody

WB Suggested Anti-CANX Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: A549 cell lysate CANX is strongly supported by BioGPS gene expression data to be expressed in Human A549 cells Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

Calnexin antibody

WB Suggested Anti-CANX Antibody; Titration: 1.0 ug/ml; Positive Control: Fetal Liver Rabbit IgG Purified Bov, Can, Eq, GP, Gt, Hu, Ms, Por, Rb, Rt, Ye, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

Calnexin (C-term) antibody

Calnexin Goat IgG Aff - Purified Can, Hu, Mky, Ms, Rt C, ICC/IF, P, WB
0.4 mg / €320.00
  SICGEN

Calnexin (C-term) antibody

Calnexin Goat IgG Aff - Purified Can, Hu, Mky, Ms, Rt C, ICC/IF, P, WB
1.0 mg / €450.00
  SICGEN

Calnexin (C-term) antibody

Calnexin Goat IgG Aff - Purified Can, Hu, Mky, Ms, Rt C, ICC/IF, P, WB
20 µl / €50.00
  SICGEN

Calnexin (C-term) antibody

Calnexin Goat IgG Aff - Purified Can, Hu, Mky, Ms, Rt C, ICC/IF, P, WB
0.4 mg / €320.00
  SICGEN

Calnexin (C-term) antibody

Calnexin Goat IgG Aff - Purified Can, Hu, Mky, Ms, Rt C, ICC/IF, P, WB
1.0 mg / €450.00
  SICGEN

Calnexin antibody

Confocal immunofluorescence analysis of Hela cells using Calnexin antibody Cat.-No AM06218SU-N (green). Mouse IgG2b 3H4A7 Ascites Hu E, ICC/IF, WB
0.1 ml / €460.00
  Acris Antibodies GmbH

Calnexin antibody

Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG2b 1D4 Purified Hu E, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

Calnexin antibody

Immunohistochemistry analysis of Calnexin antibody (Cat.-No AP06425PU-N) in paraffin-embedded human lung carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Calnexin antibody

Thyroid, Human: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Purified Can P, WB
50 µg / €470.00
  Acris Antibodies GmbH

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