Calpain-1 antibody

Principal name

Calpain-1 antibody

Alternative names for Calpain-1 antibody

Calpain mu-type, muCANP, CAPN1, CANPL1, PIG30, CANP1, Calpain-1 catalytic subunit, Calpain-1 large subunit, Calcium-activated neutral proteinase 1, Micromolar-calpain, Cell proliferation-inducing gene 30 protein

SwissProt ID

O35350 (Mouse), P06815 (Rabit), P07384 (Human), P97571 (Rat), Q27970 (Bovin)

Gene ID

823 (CAPN1), 29153 (Capn1)

Available reactivities

Bov (Bovine), Hu (Human), Ms (Mouse), Rt (Rat), Por (Porcine), Rb (Rabbit), Hst (Hamster), Eq (Equine), Gt (Goat), Sh (Sheep), Ze (Zebrafish), Can (Canine), GP (Guinea Pig)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), Frozen Sections (C), Flow Cytometry (F)

Background of Calpain-1 antibody

The calpain (calcium-dependent protease or calcium-activated neutral protease) system consists of two ubiquitous forms of calpain (mu-calpain and m-calpain), a tissue specific calpain (n-calpain), and a calpain inhibitory protein (calpastatin). The calpain system has been detected in every vertebrate tissue examined, and has been suggested to play a regulatory role in cellular protein metabolism. This regulatory role may have important implications in platelet aggregation and pathologies associated with altered calcium homeostasis and protein metabolism such as ischemic cell injury and degenerative diseases. Inhibitors of calpain have been shown to block dexamethasone and low-level irradiation induced apoptosis in thymocytes suggesting that calpain has a regulatory or mechanistic role in apoptotic cell death.
Mu- and m-calpains are heterodimers consisting of 28 kDa and 80 kDa subunits. The 28 kDa subunit is identical in the two isoforms, but the 80 kDa subunits differ with ~50% sequence similarity. 28 kDa/80 kDa complexes are thought to be inactive proenzymes which, upon binding of calcium, undergo conformational changes that promotes cleavage of the 28 kDa subunit and results in enzyme activation.

General readings

1. Calpains: Markers of tumor aggressiveness?
Roumes H, Leloup L, Dargelos E, Brustis JJ, Daury L, Cottin P
Experimental cell research 2010.
2. Changes in Hsp60 level of the failing heart following acute myocardial infarction and the effect of long-term treatment with trandolapril.
Wakako Toga,Kouichi Tanonaka,Satoshi Takeo
Biological & pharmaceutical bulletin 30 2007.
3. Possible involvement of calpain activation in pathogenesis of chronic heart failure after acute myocardial infarction.
Masaya Takahashi, Kouichi Tanonaka, Hiroyuki Yoshida, Miki Koshimizu, Takuya Daicho, Ryo Oikawa, Satoshi Takeo
Journal of cardiovascular pharmacology 47 413-21 2006.
4. Subcellular localization and in vivo subunit interactions of ubiquitous mu-calpain.
Shirley Gil-Parrado, Oliver Popp, Tobias A Knoch, Stefan Zahler, Felix Bestvater, Marcel Felgenträger, Andreas Holloschi, Amaury Fernández-Montalván, Ennes A Auerswald, Hans Fritz, Pablo Fuentes-Prior, Werner Machleidt, Eberhard Spiess
The Journal of biological chemistry 278 16336-46 2003.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Calpain-1 antibody

AP32722PU-N Calpain-1 Antibody staining of Human Spleen lysate at 0.3µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu WB
0.1 mg / €340.00
  Acris Antibodies GmbH

Calpain-1 (468-481) antibody

AP32723PU-N Calpain-1 Antibody staining of Human Kidney lysate at  0.3µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Hu, Por WB
0.1 mg / €340.00
  Acris Antibodies GmbH

Calpain-1 antibody

Western blot analysis of Calpain 1 expression in lysate from Jurkat cells treated with (Lane 2) or without (Lane 1) camptothecin. Rabbit Aff - Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Calpain-1 antibody

Western blot analysis of Human fetal kidney tissue using TA324115(CAPN1 Antibody) at dilution 1/500 Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Calpain-1 antibody

Western blot analysis of 293T cell using TA324116(CAPN1 Antibody) at dilution 1/450 Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Calpain-1 antibody

Western blot analysis of extracts of various cell lines, using CAPN1 antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Calpain-1 antibody

CAPN1 antibody validated by WB using HT1080 cell lysate at 1ug/mL. Rabbit IgG Purified Bov, Eq, Gt, Hu, Ms, Por, Rb, Rt, Sh, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

Calpain-1 antibody

WB Suggested Anti-CAPN1 Antibody; Titration: 1.0 ug/ml; Positive Control: Fetal Brain Rabbit IgG Purified Bov, Can, Eq, GP, Gt, Hu, Ms, Rb, Rt, Sh, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

Calpain-1 antibody

Calpain-1 Mouse IgG1 15C10 Purified Bov, Hu, Ms, Rt E, IP, P, WB
0.1 mg / €360.00
  Acris Antibodies GmbH

Calpain-1 antibody

CAPN1 polyclonal antibody (A01), Lot # 050913JC01 Western Blot analysis of CAPN1 expression in Jurkat ( Cat # L017V1 ). Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

Calpain-1 antibody

Immunofluorescence of purified MaxPab antibody to CAPN1 on HeLa cell. [antibody concentration 10 ug/ml] Mouse Purified Hu E(detection), ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

Calpain-1 antibody

Calpain-1 Rabbit Serum Hu E(detection), IP, WB
0.1 ml / €380.00
  Abnova Taiwan Corp.

Calpain-1 antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between CAPN1 and F2R. HeLa cells were stained with anti-CAPN1 rabbit purified polyclonal 1:1200 and anti-F2R mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

Calpain-1 antibody

Calpain-1 Mouse IgG1 15C10 Bov, Hu, Ms, Rt E, IP, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

Calpain-1 antibody

Western blot analysis of Capn1 expression in lysate from Jurkat cells treated with ( Lane 2 ) or without ( Lane 1 ) camptothecin. Rabbit Bov, Hu, Ms, Rb, Rt WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

Calpain-1 antibody

Human HT1080; CAPN1 antibody - middle region (ARP60936_P050) validated by WB using HT1080 cell lysate at 1ug/mL.; CAPN1 antibody - middle region (ARP60936_P050) in Human HT1080 cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

Calpain-1 (N-term) antibody

Human Fetal Brain; WB Suggested Anti-CAPN1 Antibody. . Titration: 1.0 ug/ml. . Positive Control: Fetal Brain; CAPN1 antibody - N-terminal region (ARP60937_P050) in Human Fetal Brain cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

Calpain-1 antibody

Calpain-1 Rabbit IgG Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €290.00
  Boster Immunoleader

Calpain-1 antibody

Calpain-1 Mouse IgG1 107-82 Ascites Hu, Ms, Por, Rb, Rt C, E, P, WB
0.1 ml / €400.00
  Novus Biologicals Inc.

Calpain-1 antibody

Western Blot: Calpain 1 Antibody (9A4H8D3) [NB120-3589] - Figure 1 shows a Western blot of mu-calpain in mouse lung extract using NB120-3589. Mouse IgG1 9A4H8D3 Ascites Bov, Hu, Ms, Por, Rb, Rt ICC/IF, WB
0.1 ml / €400.00
  Novus Biologicals Inc.

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