Calpain-1 antibody

Principal name

Calpain-1 antibody

Alternative names for Calpain-1 antibody

Calpain mu-type, muCANP, CAPN1, CANPL1, PIG30, CANP1, Calpain-1 catalytic subunit, Calpain-1 large subunit, Calcium-activated neutral proteinase 1, Micromolar-calpain, Cell proliferation-inducing gene 30 protein

SwissProt ID

O35350 (Mouse), P06815 (Rabit), P07384 (Human), P97571 (Rat), Q27970 (Bovin)

Gene ID

823 (CAPN1), 29153 (Capn1), 12333

Available reactivities

Hu (Human), Bov (Bovine), Ms (Mouse), Rt (Rat), Rb (Rabbit), Por (Porcine), Eq (Equine), Gt (Goat), Sh (Sheep), Ze (Zebrafish), Can (Canine), GP (Guinea Pig)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of Calpain-1 antibody

Calpain is a calcium-dependent thiol proteise that is functiolly active as a heterodimer composed of a small regulatory subunit and one of at least two large catalytic subunits (calpain 1 or calpain 2). In vitro, calpain 1 (mu-calpain) requires micromolar levels of calcium, while calpain 2 (M-calpain) requires millimolar levels of calcium for activation. The regulation of calpain in vivo is the subject of many current studies, which suggest that proteolytic activity is regulated post-transcriptiolly by mechanisms such as calcium requirements, subcellular localization of the heterodimer, phosphorylation via the EGFR-Erk sigling cascade, endogenous inhibitors (calpastatin) and autoproteolytic cleavage. Calpastatin negatively regulates autoproteolytic cleavage of calpain 1 between Gly27 and Leu28. Calpain influences cell migration by modifying rather than degrading its substrates responsible for cell adhesion and cytoskeletal arrangement. Control of calpain activity has caught the attention of drug development since limiting its activity could mute invasiveness of tumors or chronic inflammation.

18 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Calpain-1 antibody

AP32722PU-N Calpain-1 Antibody staining of Human Spleen lysate at 0.3µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Calpain-1 (468-481) antibody

AP32723PU-N Calpain-1 Antibody staining of Human Kidney lysate at  0.3µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Hu, Por WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Calpain-1 antibody

  Rabbit polyclonal anti-Calpain 1 antibody  
Western blot analysis of Calpain 1 expression in lysate from Jurkat cells treated with (Lane 2) or without (Lane 1) camptothecin. Rabbit Aff - Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Calpain-1 antibody

  Anti-CAPN1 Rabbit Polyclonal Antibody  
Western blot analysis of Human fetal kidney tissue using TA324115(CAPN1 Antibody) at dilution 1/500 Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Calpain-1 antibody

  Anti-CAPN1 Rabbit Polyclonal Antibody  
Western blot analysis of 293T cell using TA324116(CAPN1 Antibody) at dilution 1/450 Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Calpain-1 antibody

  Rabbit anti-CAPN1 Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using CAPN1 antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Calpain-1 antibody

  Rabbit Polyclonal Anti-CAPN1 Antibody  
CAPN1 antibody validated by WB using HT1080 cell lysate at 1ug/mL. Rabbit IgG Purified Bov, Eq, Gt, Hu, Ms, Por, Rb, Rt, Sh, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

Calpain-1 antibody

  Rabbit Polyclonal Anti-CAPN1 Antibody  
WB Suggested Anti-CAPN1 Antibody; Titration: 1.0 ug/ml; Positive Control: Fetal Brain Rabbit IgG Purified Bov, Can, Eq, GP, Gt, Hu, Ms, Rb, Rt, Sh, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

Calpain-1 antibody

Calpain-1 Mouse IgG1 15C10 Purified Bov, Hu, Ms, Rt E, IP, P, WB
0.1 mg / €370.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

CAPN1 - human gene knockout kit via CRISPR

CAPN1 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

CAPN1 - human gene knockout kit via CRISPR

CAPN1 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

CAPN1 - human gene knockout kit via CRISPR

CAPN1 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

CAPN1 - human gene knockout kit via CRISPR

CAPN1 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

CAPN1 overexpression lysate

CAPN1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, CAPN1 (mGFP-tagged) - Human calpain 1, (mu/I) large subunit (CAPN1), transcript variant 2, 200 uL, >10^7 TU/mL

Lenti ORF particles, CAPN1 (mGFP-tagged) - Human calpain 1, (mu/I) large subunit (CAPN1), transcript variant 2, 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

Lenti ORF particles, CAPN1 (Myc-DDK tagged) - Human calpain 1, (mu/I) large subunit (CAPN1), transcript variant 2 , 200 uL, >10^7 TU/mL

Lenti ORF particles, CAPN1 (Myc-DDK tagged) - Human calpain 1, (mu/I) large subunit (CAPN1), transcript variant 2 , 200 uL, >10^7 TU/mL  
200 µl / €940.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Capn1 (GFP-tagged) - Mouse calpain 1 (Capn1), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, Capn1 (GFP-tagged) - Mouse calpain 1 (Capn1), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €1,400.00
  OriGene Technologies, Inc.

Lenti ORF particles, Capn1 (Myc-DDK-tagged) - Mouse calpain 1 (Capn1), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, Capn1 (Myc-DDK-tagged) - Mouse calpain 1 (Capn1), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €1,260.00
  OriGene Technologies, Inc.

18 Item(s)

per page
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