Calpain-2 antibody

Principal name

Calpain-2 antibody

Alternative names for Calpain-2 antibody

Calpain M-type, M-calpain, CAPN2, CANPL2, CANP2, Calpain-2 catalytic subunit, Calpain-2 large subunit, Calcium-activated neutral proteinase 2, Millimolar-calpain, Calpain large polypeptide L2, Calcium-activated neutral proteinase 2

SwissProt ID

O08529 (Mouse), P17655 (Human), P43367 (Pig), Q07009 (Rat), Q27971 (Bovin)

Gene ID

824 (CAPN2)

Available reactivities

Bov (Bovine), Hu (Human), Por (Porcine), Rt (Rat), Ms (Mouse), Can (Canine), Mky (Monkey)

Available hosts

Mouse, Rabbit

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Flow Cytometry (F), Enzyme Immunoassay (E), Immunoprecipitation (IP), Paraffin Sections (P), ELISA (detection) (E(detection))

Background of Calpain-2 antibody

Mu Calpain, and m calpain, also known as Calpain 2, are intracellular, calcium dependent cysteine proteases. Mu calpain has a micromolar sensitivity (thus the mu) as compared to the millimolar calcium sensitivity of m calpain. Both Calpains 1 and 2 are composed of an 80 kD subunit and a 30 kD subunit. Whereas the 30 kDa subunit is shared by both enzymes, the larger catalytic subunits are different and exhibit the distinct Ca++ requirements that are suggested by their names. The calpains have papain like activity, thus the pain nomenclature. Both Calpain 1 and Calpain 2 are ubiquitously expressed, and are countered by the endogenous calpain inhibitor, calpastatin.Other calpain family members (calpain 94, ncl2, ncl3, etc) have more limited tissue distribution, and perhaps different functions. The calpain family members consist of a common small subunit (Calpain 4), and a large variable subunit. It is not clear that all calpains contain a small subunit. Domains in the large subunit include the amino terminal domain I, the proteinase domain II, domain III, and the EF hand domain IV. The calpains appear to serve multiple physiological roles, and ideas concerning the functions of these enzymes are in a state of rapid flux.

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12 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Calpain-2 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-CAPN2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA504280) Mouse IgG2a OTI3G1 Purified Can, Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Calpain-2 antibody

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CAPN2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human). Mouse IgG2a OTI1F4 Purified Hu, Mky, Ms, Rt F, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

Calpain-2 antibody

Predicted band size: 80kDa. Positive control: Mouse brain and skin tissue, 293T cell lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lane1: Mouse brain tissue lysate Lane2: 293T cell lysate Lane3: Mouse skin tissue lysate Lysates: 30 ug per lane Primary antibody: 1/200 dilution Secondary antibody: Donkey anti Rabbit IgG - H&L (HRP) at 1/3000 dilution Exposure time: 40 seconds) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Calpain-2 antibody

Predicted band size: 80kDa. Positive control: 293T cell and Mouse skin tissue lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lane1: 293T cell lysate Lane2: Mouse skin tissue lysate Lysates: 30 ug per lane Primary antibody: 1/200 dilution Secondary antibody: Donkey anti Rabbit IgG - H&L (HRP) at 1/3000 dilution Exposure time: 50 seconds) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Calpain-2 antibody

Calpain-2 Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 ml / €390.00
  Acris Antibodies GmbH

Calpain-2 antibody

Calpain-2 Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

Calpain-2 antibody

Calpain-2 Rabbit Hu, Ms, Rt WB
0.1 ml / €470.00
  Abnova Taiwan Corp.

Calpain-2 antibody

Western Blot: Calpain 2 Antibody (EPR2562Y) [NBP1-40458] - Western blot analysis on HeLa cell lysate using anti-Calpain2, dilution 1:500. Rabbit IgG EPR2562Y Hu F, IP, WB
0.1 ml / €480.00
  Novus Biologicals Inc.

Calpain-2 antibody

Immunohistochemistry-Paraffin: Calpain 2 Antibody [NBP1-88204] - Immunohistochemical staining of human urinary bladder shows moderate positivity in subsets of urothelial cells. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Calpain-2

Calpain-2 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

Calpain-2

Calpain-2 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

Calpain-2

Calpain-2 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

12 Item(s)

per page
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