Calpastatin antibody

Principal name

Calpastatin antibody

Alternative names for Calpastatin antibody

Calpain inhibitor, Sperm BS-17 component, CAST

SwissProt ID

P12675 (Pig), P20810 (Human), P20811 (Bovin), P51125 (Mouse)

Gene ID

831 (CAST)

Available reactivities

Hu (Human), Ms (Mouse)

Available hosts

Mouse, Rabbit

Available applications

ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Flow Cytometry (F)

Background of Calpastatin antibody

Calpastatin is an endogenous calpain (calcium-dependent cysteine protease) inhibitor. It consists of an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1-4), and it is involved in the proteolysis of amyloid precursor protein. The calpain/calpastatin system is involved in numerous membrane fusion events, such as neural vesicle exocytosis and platelet and red-cell aggregation. The protein is also thought to affect the expression levels of genes encoding structural or regulatory proteins. The calpain system has been detected in every vertebrate tissue examined, and has been suggested to play a regulatory role in cellular protein metabolism. This regulatory role may have important implications in platelet aggregation and pathologies associated with altered calcium homeostasis and protein metabolism such as ischemic cell injury and degenerative diseases. Inhibitors of calpain have been shown to block dexamethasone and low-level irradiation induced apoptosis in thymocytes suggesting that calpain has a regulatory or mechanistic role in apoptotic cell death. Calpastatin, a specific endogenous inhibitor of calpain, has a predicted molecular weight of ~77kDa and an internal repeat of four homologous domains which allow it to inhibit multiple calpain molecules simultaneously. It functions by binding to calpain when calpain binds calcium. When calpastatin is subjected to proteolytic digestion, fragments of calpastatin as small as 15kDa still retain inhibitory activity.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Calpastatin antibody

  CAST (Calpastatin) mouse monoclonal antibody, clone OTI1E7 (formerly 1E7)  
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-CAST mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504351) Mouse IgG1 OTI1E7 Purified Hu F, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

Calpastatin antibody

  Rabbit Polyclonal Anti-CAST Antibody  
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane: Lovo cells, Primary antibody: (CAST Antibody) at dilution 1/750, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 7 seconds Rabbit IgG Purified Hu E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Calpastatin antibody

Immunofluorescence of purified MaxPab antibody to CAST on HeLa cell. [antibody concentration 10 ug/ml] Mouse Serum Hu E(detection), ICC/IF, WB
50 µl / €370.00
  Abnova Taiwan Corp.

Calpastatin antibody

Calpastatin Mouse Purified Hu E(detection), ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

Calpastatin antibody

Western Blot analysis of CAST expression in transfected 293T cell line (H00000831-T02) by CAST MaxPab polyclonal antibody.

Lane 1: CAST transfected lysate(75.46 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

Calpastatin antibody

Calpastatin Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

Calpastatin antibody

Western Blot analysis of CAST expression in transfected 293T cell line (H00000831-T03) by CAST MaxPab polyclonal antibody.

Lane 1: CAST transfected lysate(74.10 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

Calpastatin antibody

CAST monoclonal antibody (M03), clone 2C5. Western Blot analysis of CAST expression in NIH/3T3 ( Cat # L018V1 ). Mouse IgG1 2C5 Purified Hu, Ms E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

CRISPR

Catalog No. Species Donor Vector  

CAST - human gene knockout kit via CRISPR

CAST - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

CAST - human gene knockout kit via CRISPR

CAST - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

CAST - human gene knockout kit via CRISPR

CAST - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

CAST - human gene knockout kit via CRISPR

CAST - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Calpastatin

Calpastatin Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

Calpastatin

Calpastatin Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

Calpastatin

Calpastatin Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

Calpastatin

Calpastatin Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

Antibody Pairs

Catalog No.    

CAST (Human) Matched Antibody Pair

CAST (Human) Matched Antibody Pair  
1 set / €710.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

CAST 293T Cell Transient Overexpression Lysate(Denatured)

CAST 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

CAST 293T Cell Transient Overexpression Lysate(Denatured)

CAST 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

Lysates (Knockout)

Catalog No.    

CAST-knockout Cell lysate

CAST-knockout Cell lysate  
0.1 mg / €495.00
  OriGene Technologies, Inc.

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