Calponin-2 antibody

Principal name

Calponin-2 antibody

Alternative names for Calponin-2 antibody

Calponin H2, Neutral calponin, CNN2

SwissProt ID

Q08093 (Mouse), Q08094 (Pig), Q3SYU6 (Bovin), Q99439 (Human)

Gene ID

1265 (CNN2)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Chk (Chicken), Ms (Mouse), Por (Porcine), Rt (Rat), Ze (Zebrafish), African clawed frog, Tr (Turkey), Mky (Monkey), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Mouse, Rabbit, Goat

Available applications

ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P)

Background of Calponin-2 antibody

Calponin regulates smooth muscle cell contraction and is a marker of smooth muscle cell differentiation. Calponin, an Actin- and Tropomyosin-binding protein, is characterized as an inhibitory factor of smooth-muscle actomyosin activity. Calponin is implicated in the regulation of smooth muscle contraction through its interaction with F-Actin and inhibition of the Actin-activated MgATPase activity of phosphorylated myosin. Both properties are lost following phosphorylation (primarily at Serine 175) by protein kinase C or calmodulin-dependent protein kinase II. The three forms of Calponin, Calponin 1 (basic Calponin), Calponin 2 (neutral Calponin) and Calponin 3 (acidic Calponin) are found in smooth muscle tissue. Additionally, Calponin 2 is found in heart muscle tissue and Calponin 3 is found in the brain.

General readings

Masuda H, Tanaka K, Takagi M, Ohgami K, Sakamaki T, Shibata N, Takahashi K,
Molecular cloning and characterization of human non-smooth muscle calponin.
Journal of biochemistry 1996 Aug 120 (2): 415-24.
PMID: 8889829

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Calponin-2 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-CNN2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503688) Mouse IgG1 OTI2B5 Purified Hu, Mky ICC/IF, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

Calponin-2 antibody

TA305763 (0.03ug/ml) staining of HepG2 lysates (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Calponin-2 antibody

Western blot analysis of extracts from HUVEC cells, using CNN2 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Calponin-2 antibody

WB Suggested Anti-CNN2 Antibody Titration: 0.2-1 ug/ml; Positive Control: HepG2 cell lysateCNN2 is supported by BioGPS gene expression data to be expressed in HepG2 Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

Calponin-2 antibody

WB Suggested Anti-CNN2 Antibody Titration: 0.2-1 ug/ml; Positive Control: 721_B cell lysateCNN2 is supported by BioGPS gene expression data to be expressed in 721_B Rabbit IgG Purified Bov, Can, GP, Hu, Ms, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

Calponin-2 (N-term) antibody

CNN2 Antibody (N-term) (Cat. #AP50987PU-N)immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CNN2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Calponin-2 antibody

Immunofluorescence of purified MaxPab antibody to CNN2 on HeLa cell. [antibody concentration 10 ug/ml] Mouse Serum Hu E(detection), ICC/IF, WB
50 µl / €370.00
  Abnova Taiwan Corp.

Calponin-2 antibody

Immunofluorescence of purified MaxPab antibody to CNN2 on HeLa cell. [antibody concentration 10 ug/ml] Mouse Purified Hu E(detection), ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

Calponin-2 antibody

Western Blot analysis of CNN2 expression in transfected 293T cell line (H00001265-T02) by CNN2 MaxPab polyclonal antibody.

Lane 1: CNN2 transfected lysate(29.50 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

Calponin-2 antibody

Immunofluorescence analysis of A549 cells, using CNN2 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, ICC/IF, WB
50 µg / €205.00
  Assay Biotech

Calponin-2 antibody

Immunofluorescence analysis of A549 cells, using CNN2 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, ICC/IF, WB
0.1 mg / €310.00
  Assay Biotech

Calponin-2 antibody

Human HepG2; WB Suggested Anti-CNN2 Antibody Titration: 0.2-1 ug/ml. Positive Control: HepG2 cell lysate. CNN2 is supported by BioGPS gene expression data to be expressed in HepG2.; CNN2 antibody - N-terminal region (ARP52039_P050) in Human HepG2 cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Por, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

Calponin-2 antibody

Human 721_B; WB Suggested Anti-CNN2 Antibody Titration: 0.2-1 ug/ml. Positive Control: 721_B cell lysate. CNN2 is supported by BioGPS gene expression data to be expressed in 721_B.; CNN2 antibody - N-terminal region (ARP52040_P050) in Human 721_B cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Por, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

Calponin-2 antibody

Calponin-2 Mouse IgG1 CP-19 Ascites Chk, Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €290.00
  Boster Immunoleader

Calponin-2 antibody

Western Blot: Calponin 2 Antibody [NBP1-36741] - (0.03µg/ml) staining of HepG2 lysates (35µg protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

Calponin-2 antibody

Calponin-2 Rabbit IgG Aff - Purified Hu, Ms E, ICC/IF, WB
0.1 mg / €360.00
  Novus Biologicals Inc.

Calponin-2 antibody

Calponin-2 Rabbit IgG Aff - Purified Hu, Ms E, ICC/IF, WB
50 µg / €290.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Calponin-2 (transcript variant 1)

Calponin-2 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Calponin-2 (transcript variant 2)

Calponin-2 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Calponin-2 (1-131, His-tag)

Calponin-2 Human Purified > 95 % by SDS - PAGE E. coli
0.5 mg / €730.00
  Acris Antibodies GmbH

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