Calreticulin antibody

Principal name

Calreticulin antibody

Alternative names for Calreticulin antibody

CRP55, Calregulin, HACBP, ERp60, grp60, CALR, CRTC

SwissProt ID

P14211 (Mouse), P18418 (Rat), P27797 (Human), P28490 (Canfa), P28491 (Pig)

Gene ID

811 (CALR), 12317 (Calr)

Available reactivities

Can (Canine), Hu (Human), Ms (Mouse), Por (Porcine), Rb (Rabbit), Rt (Rat), Mky (Monkey), Bov (Bovine), Hst (Hamster), Chk (Chicken), GP (Guinea Pig), Sh (Sheep), Prim (Primate), Ze (Zebrafish)

Available hosts

Rabbit, Mouse, Goat, Sheep

Available applications

Western blot / Immunoblot (WB), Immunoprecipitation (IP), Paraffin Sections (P), Frozen Sections (C), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Flow Cytometry (F)

Background of Calreticulin antibody

Calreticulin, also known as RO, CRT, SSA, cC1qR, FLJ26680, CALR. Entrez Protein NP_004334. It is a multifunctional protein that acts as a major Ca(2+)-binding (storage) protein in the lumen of the endoplasmic reticulum. It is also found in the nucleus, suggesting that it may have a role in transcription regulation. Calreticulin binds to the synthetic peptide KLGFFKR, which is almost identical to an amino acid sequence in the DNA-binding domain of the superfamily of nuclear receptors. Calreticulin binds to antibodies in certain sera of systemic lupus and Sjogren patients which contain anti-Ro/SSA antibodies, it is highly conserved among species, and it is located in the endoplasmic and sarcoplasmic reticulum where it may bind calcium. The amino terminus of calreticulin interacts with the DNA-binding domain of the glucocorticoid receptor and prevents the receptor from binding to its specific glucocorticoid response element. Calreticulin can inhibit the binding of androgen receptor to its hormone-responsive DNA element and can inhibit androgen receptor and retinoic acid receptor transcriptional activities in vivo, as well as retinoic acid-induced neuronal differentiation. Thus, calreticulin can act as an important modulator of the regulation of gene transcription by nuclear hormone receptors. Systemic lupus erythematosus is associated with increased autoantibody titers against calreticulin but calreticulin is not a Ro/SS-A antigen. Earlier papers referred to calreticulin as an Ro/SS-A antigen but this was later disproven. Increased autoantibody titer against human calreticulin is found in infants with complete congenital heart block of both the IgG and IgM classes.

General readings

Kropp LE., et al. (2010) J Immunol. 184(10):5619-27.
Singh P., et al. (2010) J Biochem. 147(5):625-32.

  1. Quick-Filters:
  2. Highly Validated (4)

Items 1 to 20 of 43 total

per page
Page:
  1. 1
  2. 2
  3. 3

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Calreticulin antibody

Western Blot: Calreticulin Antibody (1G6A7) [TA336776] - Western blot analysis using anti-Calreticulin mAb against Hela (1), A549 (2), NTERA2 (3) and MCF-7 (4) cell lysate. Mouse IgG2a 1G6A7 Purified Hu, Ms C, E, F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Calreticulin antibody

Formalin-Fixed, Paraffin-Embedded Human Placenta stained with CALR antibody Cat.-No AM06211SU-N followed by biotinylated anti-Mouse IgG secondary antibody, Alkaline Phosphatase-Streptavidin and Chromogen. Mouse IgG2a 1G6A7 Ascites Hu, Ms E, ICC/IF, P, WB
0.1 ml / €440.00
  Acris Antibodies GmbH

Calreticulin antibody

CALR Antibody (Center) (Cat.-No AP17172PU-N) western blot analysis in Jurkat, Ramos, Rat C6 cell line lysates (35ug/lane). This demonstrates the CALR antibody detected the CALR protein (arrow). Rabbit Aff - Purified Hu, Rt E, F, P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Calreticulin antibody

CALR Antibody (Cat.-No AP33519PU-N) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CALR Antibody for immunohistochemistry. Clinical relevance has not been evaluated. Rabbit Aff - Purified Hu, Rt E, F, P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Calreticulin antibody

Western blot analysis of Calreticulin in various cell lysates, using a 1:1000 dilution of TA309300. Rabbit IgG Serum Bov, Can, Chk, GP, Hst, Hu, Mky, Ms, Por, Rb, Rt, Sh C, ICC/IF, IP, WB
0.2 ml / €325.00
  OriGene Technologies, Inc.

Calreticulin antibody

Western blot analysis of extracts from COS-7 cells, using CALR antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Calreticulin antibody

Predicted band size: 48 kDa . Positive control: NIH/3T3 cell lysate. Recommended dilution: 1/1000-5000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/800 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 3 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Calreticulin antibody

Predicted band size: 48 kDa . Positive control: Hela, 293T and NIH/3T3 cell lysate. Recommended dilution: 1/1000-5000. (Gel: 10%SDS-PAGE Lane1: Hela cell lysate Lane2: 293T cell lysate Lane3: NIH/3T3 cell lysate Lysates: 40 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 30 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Calreticulin antibody

Western blot analysis of Hela cell and HepG2 cell lysate using CALR antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

Calreticulin antibody

Anti-CALR antibody (0.01µg/ml) staining of Human (A) and Rat (B) Lung lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Calreticulin antibody

Western Blot: Calreticulin Antibody [TA336667] - Human kidney lysate. Rabbit Purified Bov, Hst, Hu, Ms, Prim, Rt C, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Calreticulin antibody

Western Blot: Calreticulin Antibody [TA336669] - Detection of calreticulin in human kidney lysate using NB 600-103 at 1:1,000. ECL exposure, 10 seconds. Rabbit Purified Hu, Ms ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Calreticulin antibody

WB Suggested Anti-CALR Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Human brain Rabbit IgG Purified Bov, Can, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

Calreticulin antibody

WB Suggested Anti-CALR Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: HepG2 cell lysateCALR is strongly supported by BioGPS gene expression data to be expressed in Human HepG2 cells Rabbit IgG Purified Bov, Can, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

Calreticulin antibody

Host: Rabbit; Target Name: CALR; Sample Tissue: 721_B Whole cell lysates; Antibody Dilution: 1.0 ug/mlThere is BioGPS gene expression data showing that CALR is expressed in 721_B Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

Calreticulin antibody

Calreticulin Mouse IgG1 S75 Purified Hu, Mky IP, P, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

Calreticulin antibody

Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG1 1G11-1A9 Purified Hu E, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

Calreticulin antibody

Calreticulin Rabbit Purified Hu, Mky, Ms, Rt P, WB
0.1 mg / €390.00
  Acris Antibodies GmbH

Calreticulin antibody

Immunofluorescence of MCF-7 cells stained with antibody at 1/50 dilution. Sheep IgG Purified Can, Hu, Ms, Rt ICC/IF, IP, P, WB
0.25 mg / €360.00
  Acris Antibodies GmbH

Calreticulin antibody

Calreticulin Goat Serum Hu C, WB
0.1 ml / €440.00
  Acris Antibodies GmbH

Items 1 to 20 of 43 total

per page
Page:
  1. 1
  2. 2
  3. 3
  • LinkedIn