CAMK2A antibody

Principal name

CAMK2A antibody

Alternative names for CAMK2A antibody

CAMKA, KIAA0968, CaM-kinase II alpha chain, CaM kinase II subunit alpha, CaMK-II subunit alpha, Calcium/calmodulin-dependent protein kinase type II alpha chain

SwissProt ID

P11275 (Rat), P11798 (Mouse), Q00168 (Drome), Q9UQM7 (Human)

Gene ID

815 (CAMK2A), 25400 (Camk2a), 12322

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Mky (Monkey), Xen (Xenopus), Bov (Bovine), Chk (Chicken), Can (Canine), Frog, Por (Porcine), Rb (Rabbit), Ze (Zebrafish), Sh (Sheep), Orang-Utan

Available hosts

Rabbit, Mouse, Goat

Available applications

Western blot / Immunoblot (WB), Frozen Sections (C), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), Dot blot (Dot), Paraffin Sections (P), Immunoprecipitation (IP), Radioimmunoassay (R)

Background of CAMK2A antibody

Ca2+/CalmodulinDependent Protein Kinase II (CaM Kinase II) is a multifunctional calcium and calmodulindependent protein kinase that mediates cellular responses to a wide variety of intercellular signals. CaM Kinase II has been shown to regulate diverse cellular functions including synaptic plasticity, neurotransmitter synthesis and release, gene expression, ion channel function, carbohydrate metabolism, cytoskeletal function, and Ca2+homeostasis. Phosphorylation of Thr286 on the kinase produces an autonomously active form of CaM Kinase II. Autophosphorylation of Thr305 inhibits the activity CaM Kinase II. Phosphorylation at this site appears to reduce the association of CaM Kinase II with the PSD and reduce LTP and learning.
CaM kinase II, in a calcium and calmodulin dependent manner, phosphorylates many different brain substrates including synapsin I, tryptophan hydroxylase, tyrosine hydroxylase and nitric oxide synthase thereby performing regulatory functions associated with increases in intracellular free calcium. CaM kinase II is particularly abundant in the hippocampus and forebrain where it comprises ~1% of total protein. Within the neuron, CaM kinase II is a major component of the postsynaptic density fraction accounting for as much as 30% of total protein in the post synaptic density (PSD) region. Autophosphorylation of threonine-286 occurs after calcium/calmodulin activation and can enable CaM kinase II to remain active independent of free calcium. Autophosphorylation of CaM kinase II in hippocampal neurons, which results in relatively high levels of kinase activity even at basal calcium concentrations, may allow for both upward and downward regulation as opposed to simply on/off regulation.

General readings

J Bossuyt, K Helmstadter, X Wu, et al. (2008) Ca2+/Calmodulin-Dependent Protein Kinase II {delta} and Protein Kinase D Overexpression Reinforce. Circulation Research, 102(6):695-702.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CAMK2A antibody

Immunofluorescence of monoclonal antibody to CAMK2A on HeLa cell. [antibody concentration 10 ug/ml] Mouse IgG2a 2C4 Purified Hu, Rt E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

CAMK2A antibody

TA305709 (0.1ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CAMK2A antibody

Western blot of rat brain lysate showing specific immunolabeling of the ~50k α- and the ~60k β-CaM Kinase II phosphorylated at Thr286 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: λ-Ptase). The blot is identical to the control except that it was incubated in λ-Ptase (1200 units for 30 min) before being exposed to the Anti-Thr286 CaM Kinase II. The immunolabeling is completely eliminated by treatment with λ-Ptase. Rabbit IgG Purified Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CAMK2A antibody

Western blot of rat brain lysate showing specific immunolabeling of the ~50k α- and the ~60k β-CaM Kinase II phosphorylated at Thr305 (Control).  The immunolabeling is blocked by the phosphopeptide used as the antigen (Peptide) but not by the corresponding dephosphopeptide (not shown). Rabbit IgG Purified Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CAMK2A antibody

IHC staining of hippocampal neurons Mouse IgG1 6G9 Purified Bov, Hu, Ms, Rt C, E, ICC/IF, IP, R, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CAMK2A antibody

Western blot analysis of extracts from NIH/3T3 cells, using CaMKII (Phospho-Thr305) Antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CAMK2A antibody

Immunohistochemistry analysis of paraffin-embedded human brain tissue using CaMK2a/ß/d antibody.The picture on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

CAMK2A (6-23) antibody

WB using Anti-CaM Kinase II antibody shows detection of a band ~54 kDa corresponding to human alpha CaM Kinase II (arrowhead lane 1). Specific reactivity with this band is blocked when the antibody is pre-incubated with the immunizing peptide (lane 2). The primary antibody diluted to 1:1,500 for 2h at RTfollowed by washes and reaction with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX  for 45 min at room temperature. Rabbit IgG Aff - Purified Can, Chk, Frog, Hu, Ms, Orang-Utan, Por, Rb, Rt, Ze E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CAMK2A antibody

Western blot analysis of CaMK2 alpha/delta expression in NIH-3T3 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CAMK2A antibody

Western blot analysis of CaMK2 phosphorylation expression in K562 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CAMK2A antibody

Western blot analysis of CaMK2 alpha/ beta/ delta phosphorylation expression in NIH-3T3 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CAMK2A antibody

CAMK2A Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CAMK2A antibody

Human Brain stained with anti-CaM Kinase Kinase alpha antibody. Rabbit IgG Purified Hu P
0.1 ml / €325.00
  OriGene Technologies, Inc.

CAMK2A antibody

IHC staining of hippocampal neurons Mouse IgG1 6G9 Purified Bov, Hu, Ms, Rt C, E, ICC/IF, IP, R, WB
25 µg / €200.00
  Acris Antibodies GmbH

CAMK2A antibody

CAMK2A Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 mg / €390.00
  Acris Antibodies GmbH

CAMK2A pThr286 antibody

Western blot of 10 μg of rat brain lysate showing specific immunolabeling of α- and β-CaM Kinase II phosphorylated at Thr286. The labeling by the antibody to CaM Kinase II Thr286 is specifically blocked by the Thr286 phosphopeptide used as antigen. The corresponding nonphosphopeptide did not block the immunolabeling (not shown). Rabbit IgG Aff - Purified Hu, Ms, Rt, Xen Dot, WB
10 Blots / €470.00
  Acris Antibodies GmbH

CAMK2A pThr305 antibody

Western blot of 10 μg of rat brain lysate showing specific immunolabeling of α- and β-CaM Kinase II phosphorylated at Thr305. The labeling by the antibody to CaM Kinase II Thr305 is specifically blocked by the Thr305 phosphopeptide used as antigen. The corresponding nonphosphopeptide did not block the immunolabeling (not shown). Rabbit cocktail Aff - Purified Bov, Chk, Hu, Ms, Rt, Xen Dot, WB
10 Blots / €470.00
  Acris Antibodies GmbH

CAMK2A pThr286 antibody

CAMK2A Rabbit IgG Purified Hu, Ms, Rt, Xen WB
0.1 ml / €510.00
  Acris Antibodies GmbH

CAMK2A pThr286 antibody

Brain, cerebellum: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu, Ms, Rt P, WB
50 µg / €470.00
  Acris Antibodies GmbH

CAMK2A (6-23) antibody

Brain, cortex: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Purified Can, Frog, Hu, Por, Rb, Rt, Ze E, ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

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