CAMK2A antibody

Principal name

CAMK2A antibody

Alternative names for CAMK2A antibody

CAMKA, KIAA0968, CaM-kinase II alpha chain, CaM kinase II subunit alpha, CaMK-II subunit alpha, Calcium/calmodulin-dependent protein kinase type II alpha chain

SwissProt ID

P11275 (Rat), P11798 (Mouse), Q00168 (Drome), Q9UQM7 (Human)

Gene ID

815 (CAMK2A), 818 (CAMK2G), 25400 (Camk2a), 94032 (CAMK2N2), 12322, 816 (CAMK2B)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Mky (Monkey), Bov (Bovine), Chk (Chicken), GP (Guinea Pig), Hst (Hamster), Rb (Rabbit), Amph (Amphibian), Can (Canine), Por (Porcine), Ze (Zebrafish), Xen (Xenopus), Frog, Orang-Utan, Sh (Sheep)

Available hosts

Rabbit, Mouse, Goat

Available applications

Western blot / Immunoblot (WB), Frozen Sections (C), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Immunoprecipitation (IP), Radioimmunoassay (R), ELISA (detection) (E(detection)), Dot blot (Dot)

Background of CAMK2A antibody

Ca2+/CalmodulinDependent Protein Kinase II (CaM Kinase II) is a multifunctional calcium and calmodulindependent protein kinase that mediates cellular responses to a wide variety of intercellular signals. CaM Kinase II has been shown to regulate diverse cellular functions including synaptic plasticity, neurotransmitter synthesis and release, gene expression, ion channel function, carbohydrate metabolism, cytoskeletal function, and Ca2+homeostasis. Phosphorylation of Thr286 on the kinase produces an autonomously active form of CaM Kinase II. Autophosphorylation of Thr305 inhibits the activity CaM Kinase II. Phosphorylation at this site appears to reduce the association of CaM Kinase II with the PSD and reduce LTP and learning.
CaM kinase II, in a calcium and calmodulin dependent manner, phosphorylates many different brain substrates including synapsin I, tryptophan hydroxylase, tyrosine hydroxylase and nitric oxide synthase thereby performing regulatory functions associated with increases in intracellular free calcium. CaM kinase II is particularly abundant in the hippocampus and forebrain where it comprises ~1% of total protein. Within the neuron, CaM kinase II is a major component of the postsynaptic density fraction accounting for as much as 30% of total protein in the post synaptic density (PSD) region. Autophosphorylation of threonine-286 occurs after calcium/calmodulin activation and can enable CaM kinase II to remain active independent of free calcium. Autophosphorylation of CaM kinase II in hippocampal neurons, which results in relatively high levels of kinase activity even at basal calcium concentrations, may allow for both upward and downward regulation as opposed to simply on/off regulation.

General readings

Abbott AL et al. Identification of a translocation deficiency in cortical granule secretion in preovulatory mouse oocytes. Biol Reprod 65:1640-7 (2001). PubMed PMID: 11717123
Erondu NE & Kennedy MB Regional distribution of type II Ca2+/calmodulin-dependent protein kinase in rat brain. J Neurosci 5:3270-7 (1985). PubMed PMID: 4078628

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CAMK2A antibody

Immunofluorescence of monoclonal antibody to CAMK2A on HeLa cell. [antibody concentration 10 ug/ml] Mouse IgG2a 2C4 Purified Hu, Rt E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

CAMK2A (487-505) antibody

Human brain stained with Anti-CaM kinase kinase alpha, cat# AP15389PU Rabbit Purified Hu, Rt P
0.5 ml / €360.00
  Acris Antibodies GmbH

CAMK2A (487-505) antibody

Human brain stained with Anti-CaM kinase kinase alpha, cat# AP15389PU Rabbit Purified Hu, Rt P
1 ml / €520.00
  Acris Antibodies GmbH

CAMK2A (487-505) antibody

Human brain stained with Anti-CaM kinase kinase alpha, cat# AP15389PU Rabbit Purified Hu, Rt P
0.1 ml / €190.00
  Acris Antibodies GmbH

CAMK2A antibody

TA305709 (0.1ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CAMK2A antibody

IHC staining of hippocampal neurons Mouse IgG1 6G9 Purified Bov, Hu, Ms, Rt C, E, ICC/IF, IP, R, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CAMK2A antibody

Immunohistochemistry analysis of paraffin-embedded human brain tissue using CaMK2a/ß/d antibody.The picture on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

CAMK2A (6-23) antibody

WB using Anti-CaM Kinase II antibody shows detection of a band ~54 kDa corresponding to human alpha CaM Kinase II (arrowhead lane 1). Specific reactivity with this band is blocked when the antibody is pre-incubated with the immunizing peptide (lane 2). The primary antibody diluted to 1:1,500 for 2h at RTfollowed by washes and reaction with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX  for 45 min at room temperature. Rabbit IgG Aff - Purified Can, Chk, Frog, Hu, Ms, Orang-Utan, Por, Rb, Rt, Ze E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CAMK2A antibody

Western blot analysis of CaMK2 alpha/delta expression in NIH-3T3 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CAMK2A antibody

CAMK2A Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CAMK2A antibody

IHC staining of hippocampal neurons Mouse IgG1 6G9 Purified Bov, Hu, Ms, Rt C, E, ICC/IF, IP, R, WB
25 µg / €200.00
  Acris Antibodies GmbH

CAMK2A antibody

CAMK2A Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 mg / €390.00
  Acris Antibodies GmbH

CAMK2A pThr286 antibody

Western blot of 10 μg of rat brain lysate showing specific immunolabeling of α- and β-CaM Kinase II phosphorylated at Thr286. The labeling by the antibody to CaM Kinase II Thr286 is specifically blocked by the Thr286 phosphopeptide used as antigen. The corresponding nonphosphopeptide did not block the immunolabeling (not shown). Rabbit IgG Aff - Purified Hu, Ms, Rt, Xen Dot, WB
10 Blots / €460.00
  Acris Antibodies GmbH

CAMK2A pThr305 antibody

Western blot of 10 μg of rat brain lysate showing specific immunolabeling of α- and β-CaM Kinase II phosphorylated at Thr305. The labeling by the antibody to CaM Kinase II Thr305 is specifically blocked by the Thr305 phosphopeptide used as antigen. The corresponding nonphosphopeptide did not block the immunolabeling (not shown). Rabbit cocktail Aff - Purified Bov, Chk, Hu, Ms, Rt, Xen Dot, WB
10 Blots / €460.00
  Acris Antibodies GmbH

CAMK2A pThr286 antibody

CAMK2A Rabbit IgG Purified Hu, Ms, Rt, Xen WB
0.1 ml / €510.00
  Acris Antibodies GmbH

CAMK2A pThr286 antibody

Brain, cerebellum: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu, Ms, Rt P, WB
50 µg / €470.00
  Acris Antibodies GmbH

CAMK2A (6-23) antibody

Brain, cortex: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Purified Can, Frog, Hu, Por, Rb, Rt, Ze E, ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

CAMK2A (C-term) antibody

Formalin-fixed and paraffin-embedded human brain tissue reacted with CAMK2 alpha Antibody (C-term) (AP13884PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CAMK2A (C-term) antibody

Formalin-fixed and paraffin-embedded human brain tissue reacted with CAMK2A (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CAMK2A (+CAMK2D) antibody

Immunohistochemistry (IHC) analyzes of CaMKIIalpha/delta antibody (Cat.-No.: AP20564PU-N) in paraffin-embedded human breast carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

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