CAND1 antibody

Principal name

CAND1 antibody

Alternative names for CAND1 antibody

KIAA0829, TIP120, TIP120A, p120 CAND1, Cullin-associated NEDD8-dissociated protein 1, Cullin-associated and neddylation-dissociated protein 1, TBP-interacting protein TIP120A, TBP-interacting protein of 120 kDa A

SwissProt ID

A7MBJ5 (Bovin), P97536 (Rat), Q6ZQ38 (Mouse), Q86VP6 (Human)

Gene ID

55832 (CAND1), 71902 (Cand1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Ze (Zebrafish), African clawed frog, Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), ELISA (detection) (E(detection))

Background of CAND1 antibody

Kit Component:
- KN302495G1, Cand1 gRNA vector 1 in pCas-Guide vector
- KN302495G2, Cand1 gRNA vector 2 in pCas-Guide vector
- KN302495D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:
CRISPR/CAS9 Genome Editing Manual

General readings

Specific Reference(s):
1. Feng S, Shen Y, Sullivan J, Rubio C, Xiong Y (2004) The Arabidopsis thaliana CAND1, an unmodified Cul1 intereacting protein, is involved in multiple devlopmen pathways controlled by ubiquitin/proteasome-mediated protein degradation. Plant Cell in press.
2. Cheng Y, Dai X, Zhao Y. (2004) AtCAND1, a HEAT-repeat protein that participates in auxin signaling in Arabidopsis. Plant Physiol. 135(2):1020-6.
3. Liu J, Furukawa M, Matsumoto T, Xiong Y.(2002) NEDD8 modification of CUL1 dissociates p120(CAND1), an inhibitor of CUL1-SKP1 binding and SCF ligases. Mol Cell. 10(6):1511-8.
4. Makino Y, et al.(1999) TATA-Binding protein-interacting protein 120, TIP120, stimulates three classes of eukaryotic transcription via a unique mechanism. Mol Cell Biol. 19(12):7951-60.
5. Makino Y, Yoshida T, Yogosawa S, Tanaka K, Muramatsu M, Tamura TA. (1999) Multiple mammalian proteasomal ATPases, but not proteasome itself, are associated with TATA-binding protein and a novel transcriptional activator, TIP120. Genes Cells. 4(9):529-39.
6. Oshikawa K, Matsumoto M, Yada M, Kamura T, Hatakeyama S, Nakayama KI. Preferential interaction of TIP120A with Cul1 that is not modified by NEDD8 and not associated with Skp1. Biochem Biophys Res Commun. 303(4):1209-16.
7. Zheng J, Yang X, Harrell JM, Ryzhikov S, Shim EH, Lykke-Andersen K, Wei N, Sun H, Kobayashi R, Zhang H. (2002) CAND1 binds to unneddylated CUL1 and regulates the formation of SCF ubiquitin E3 ligase complex. Mol Cell. 10(6):1519-26.
General Reference(s):
1. Jentsch S, Pyrowolakis G. (2000) Ubiquitin and its kin: how close are the family ties. Trends Cell Biol. 10(8):335-42.
2. Min,K.W., Hwang,J.W., Lee,J.S., Park,Y., Tamura,T.A. and Yoon,J.B. (2003 TIP120A associates with cullins and modulates ubiquitin ligase activity. J. Biol. Chem. 278 (18), 15905-15910.
3. Zheng,J., Yang,X., Harrell,J.M., Ryzhikov,S., Shim,E.H., Lykke-Andersen,K., Wei,N., Sun,H., Kobayashi,R. and Zhang,H. (2002) CAND1 binds to unneddylated CUL1 and regulates the formation of SCF ubiquitin E3 ligase complex. Mol. Cell 10 (6), 1519-1526.
4. Liu,J., Furukawa,M., Matsumoto,T. and Xiong,Y. (2002) NEDD8 modification of CUL1 dissociates p120(CAND1), an inhibitor of CUL1-SKP1 binding and SCF ligases. Mol. Cell 10 (6), 1511-1518.

16 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CAND1 antibody

  Rabbit Polyclonal Anti-CAND1 Antibody  
WB Suggested Anti-CAND1 Antibody ; Titration: 1 ug/ml; Positive Control: mouse testis Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

CAND1 antibody

  Rabbit Polyclonal Anti-CAND1 Antibody  
WB Suggested Anti-TIP120A Antibody Titration: 1.25 ug/ml; ELISA Titer: 1:62500; Positive Control: Human brain Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CAND1 (N-term) antibody

Kidney: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Serum Hu, Ms, Rt E, IP, P, WB
50 µl / €470.00
  Acris Antibodies GmbH

CAND1 antibody

Figure 1. Conjugation pathways for ubiquitin and ubiquitin-like modifiers (UBLs). Most modifiers mature by proteolytic processing from inactive precursors (a; amino acid).
Arrowheads point to the cleavage sites. Ubiquitin is expressed either as polyubiquitin or as a fusion with ribosomal proteins.
Conjugation requires activating (E1) and conjugating (E2) enzymes that form thiolesters (S) with the modifiers. Modification of cullins
by RUB involves SCF(SKP1/cullin-1/F-box protein)/CBC(cullin-2/elongin B/elongin C)-like E3 enzymes that are also involved in ubiquitination. In contrast to ubiquitin, the UBLs do not seem to form multi-UBL chains. UCRP(ISG15) resembles two ubiquitin moieties linked head-to-tail. Whether HUB1 functions as a modifier
is currently unclear. APG12 and URM1 are distinct from the other modifiers because they are unrelated in sequence to ubiquitin.
Data contributed by S. Jentsch, see references above. Rabbit Serum Hu, Ms, Rt E, IP, WB
0.1 ml / €450.00
  Acris Antibodies GmbH

CAND1 antibody

Figure 1. Conjugation pathways for ubiquitin and ubiquitin-like modifiers (UBLs). Most modifiers mature by
proteolytic processing from inactive precursors (a; amino acid).
Arrowheads point to the cleavage sites. Ubiquitin is expressed either as polyubiquitin or as a fusion with ribosomal proteins.
Conjugation requires activating (E1) and conjugating (E2) enzymes that form thiolesters (S) with the modifiers. Modification of cullins
by RUB involves SCF(SKP1/cullin-1/F-box protein) /CBC(cullin-2/elongin B/elongin C)-like E3 enzymes that are also involved in
ubiquitination. In contrast to ubiquitin, the UBLs do not seem to form multi-UBL chains. UCRP(ISG15) resembles two ubiquitin moieties linked head-to-tail. Whether HUB1 functions as a modifier
is currently unclear. APG12 and URM1 are distinct from the other modifiers because they are unrelated in sequence to ubiquitin.
Data contributed by S. Jentsch, see references above. Rabbit Serum Hu, Ms, Rt E, IP, WB
0.1 ml / €450.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

CAND1 - human gene knockout kit via CRISPR

CAND1 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

CAND1 - human gene knockout kit via CRISPR

CAND1 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

CAND1 - human gene knockout kit via CRISPR

CAND1 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

CAND1 - human gene knockout kit via CRISPR

CAND1 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Cand1 - mouse gene knockout kit via CRISPR

Cand1 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Cand1 - mouse gene knockout kit via CRISPR

Cand1 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Cand1 - mouse gene knockout kit via CRISPR

Cand1 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Cand1 - mouse gene knockout kit via CRISPR

Cand1 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

CAND1 overexpression lysate

CAND1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, CAND1 (mGFP-tagged) - Human cullin-associated and neddylation-dissociated 1 (CAND1), 200 uL, >10^7 TU/mL

Lenti ORF particles, CAND1 (mGFP-tagged) - Human cullin-associated and neddylation-dissociated 1 (CAND1), 200 uL, >10^7 TU/mL  
200 µl / €1,320.00
  OriGene Technologies, Inc.

Lenti ORF particles, CAND1 (Myc-DDK tagged) - Human cullin-associated and neddylation-dissociated 1 (CAND1) , 200 uL, >10^7 TU/mL

Lenti ORF particles, CAND1 (Myc-DDK tagged) - Human cullin-associated and neddylation-dissociated 1 (CAND1) , 200 uL, >10^7 TU/mL  
200 µl / €1,180.00
  OriGene Technologies, Inc.

16 Item(s)

per page
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