CAND1 antibody

Principal name

CAND1 antibody

Alternative names for CAND1 antibody

KIAA0829, TIP120, TIP120A, p120 CAND1, Cullin-associated NEDD8-dissociated protein 1, Cullin-associated and neddylation-dissociated protein 1, TBP-interacting protein TIP120A, TBP-interacting protein of 120 kDa A

SwissProt ID

A7MBJ5 (Bovin), P97536 (Rat), Q6ZQ38 (Mouse), Q86VP6 (Human)

Gene ID

55832 (CAND1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Ze (Zebrafish), African clawed frog, Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), ELISA (detection) (E(detection)), Frozen Sections (C)

Background of CAND1 antibody

CAND1 is also known as TIP120A, and TATA-binding protein-interacting protein 120A. The SCF complex consists of the invariable components Skp1, Cul1, and Rbx1 as well as a variable F-box protein, and functions as an E3 ubiquitin ligase. E3 ubiquitin ligases regulate various physiological processes. CAND1 binds to Cul1 and potentially regulates the SCF complex. CAND1 physically associates with Cul1 in the nucleus and this interaction is mediated by a central region of Cul1 distinct from its binding sites for Skp1 and Rbx1. CAND1 selectively binds to unneddylated CUL1 and is dissociated by CUL1 neddylation. CAND1 forms a ternary complex with CUL1 and ROC1.

General readings

Specific Reference(s):
1. Feng S, Shen Y, Sullivan J, Rubio C, Xiong Y (2004) The Arabidopsis thaliana CAND1, an unmodified Cul1 intereacting protein, is involved in multiple devlopmen pathways controlled by ubiquitin/proteasome-mediated protein degradation. Plant Cell in press.
2. Cheng Y, Dai X, Zhao Y. (2004) AtCAND1, a HEAT-repeat protein that participates in auxin signaling in Arabidopsis. Plant Physiol. 135(2):1020-6.
3. Liu J, Furukawa M, Matsumoto T, Xiong Y.(2002) NEDD8 modification of CUL1 dissociates p120(CAND1), an inhibitor of CUL1-SKP1 binding and SCF ligases. Mol Cell. 10(6):1511-8.
4. Makino Y, et al.(1999) TATA-Binding protein-interacting protein 120, TIP120, stimulates three classes of eukaryotic transcription via a unique mechanism. Mol Cell Biol. 19(12):7951-60.
5. Makino Y, Yoshida T, Yogosawa S, Tanaka K, Muramatsu M, Tamura TA. (1999) Multiple mammalian proteasomal ATPases, but not proteasome itself, are associated with TATA-binding protein and a novel transcriptional activator, TIP120. Genes Cells. 4(9):529-39.
6. Oshikawa K, Matsumoto M, Yada M, Kamura T, Hatakeyama S, Nakayama KI. Preferential interaction of TIP120A with Cul1 that is not modified by NEDD8 and not associated with Skp1. Biochem Biophys Res Commun. 303(4):1209-16.
7. Zheng J, Yang X, Harrell JM, Ryzhikov S, Shim EH, Lykke-Andersen K, Wei N, Sun H, Kobayashi R, Zhang H. (2002) CAND1 binds to unneddylated CUL1 and regulates the formation of SCF ubiquitin E3 ligase complex. Mol Cell. 10(6):1519-26.
General Reference(s):
1. Jentsch S, Pyrowolakis G. (2000) Ubiquitin and its kin: how close are the family ties. Trends Cell Biol. 10(8):335-42.
2. Min,K.W., Hwang,J.W., Lee,J.S., Park,Y., Tamura,T.A. and Yoon,J.B. (2003 TIP120A associates with cullins and modulates ubiquitin ligase activity. J. Biol. Chem. 278 (18), 15905-15910.
3. Zheng,J., Yang,X., Harrell,J.M., Ryzhikov,S., Shim,E.H., Lykke-Andersen,K., Wei,N., Sun,H., Kobayashi,R. and Zhang,H. (2002) CAND1 binds to unneddylated CUL1 and regulates the formation of SCF ubiquitin E3 ligase complex. Mol. Cell 10 (6), 1519-1526.
4. Liu,J., Furukawa,M., Matsumoto,T. and Xiong,Y. (2002) NEDD8 modification of CUL1 dissociates p120(CAND1), an inhibitor of CUL1-SKP1 binding and SCF ligases. Mol. Cell 10 (6), 1511-1518.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CAND1 antibody

CAND1 polyclonal antibody (A01), Lot # 051005JC01. Western Blot analysis of CAND1 expression in NIH/3T3. Mouse Serum Hu, Ms, Rt E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

CAND1 antibody

Immunoperoxidase of monoclonal antibody to CAND1 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml] Mouse IgG1 5D7 Purified Hu E, ICC/IF, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

CAND1 antibody

CAND1 monoclonal antibody (M02), clone 5F2. Western Blot analysis of CAND1 expression in NIH/3T3(Cat # L018V1 ). Mouse IgG3 5F2 Purified Hu, Ms, Rt E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

CAND1 antibody

CAND1 monoclonal antibody (M03), clone 2D7. Western Blot analysis of CAND1 expression in NIH/3T3(Cat # L018V1 ). Mouse IgG3 2D7 Purified Hu, Ms, Rt E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

CAND1 antibody

Immunofluorescence of monoclonal antibody to CAND1 on HeLa cell . [antibody concentration 10 ug/ml] Mouse IgG3 4D10 Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

CAND1 antibody

CAND1 monoclonal antibody (M05), clone 1G5. Western Blot analysis of CAND1 expression in NIH/3T3(Cat # L018V1 ). Mouse IgG3 1G5 Purified Hu, Ms, Rt E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

CAND1 antibody

WB Suggested Anti-CAND1 Antibody ; Titration: 1 ug/ml; Positive Control: mouse testis Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

CAND1 antibody

WB Suggested Anti-TIP120A Antibody Titration: 1.25 ug/ml; ELISA Titer: 1:62500; Positive Control: Human brain Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CAND1 (N-term) antibody

Kidney: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Serum Hu, Ms, Rt E, IP, P, WB
50 µl / €470.00
  Acris Antibodies GmbH

CAND1 antibody

Figure 1. Conjugation pathways for ubiquitin and ubiquitin-like modifiers (UBLs). Most modifiers mature by proteolytic processing from inactive precursors (a; amino acid).
Arrowheads point to the cleavage sites. Ubiquitin is expressed either as polyubiquitin or as a fusion with ribosomal proteins.
Conjugation requires activating (E1) and conjugating (E2) enzymes that form thiolesters (S) with the modifiers. Modification of cullins
by RUB involves SCF(SKP1/cullin-1/F-box protein)/CBC(cullin-2/elongin B/elongin C)-like E3 enzymes that are also involved in ubiquitination. In contrast to ubiquitin, the UBLs do not seem to form multi-UBL chains. UCRP(ISG15) resembles two ubiquitin moieties linked head-to-tail. Whether HUB1 functions as a modifier
is currently unclear. APG12 and URM1 are distinct from the other modifiers because they are unrelated in sequence to ubiquitin.
Data contributed by S. Jentsch, see references above. Rabbit Serum Hu, Ms, Rt E, IP, WB
0.1 ml / €440.00
  Acris Antibodies GmbH

CAND1 antibody

Figure 1. Conjugation pathways for ubiquitin and ubiquitin-like modifiers (UBLs). Most modifiers mature by
proteolytic processing from inactive precursors (a; amino acid).
Arrowheads point to the cleavage sites. Ubiquitin is expressed either as polyubiquitin or as a fusion with ribosomal proteins.
Conjugation requires activating (E1) and conjugating (E2) enzymes that form thiolesters (S) with the modifiers. Modification of cullins
by RUB involves SCF(SKP1/cullin-1/F-box protein) /CBC(cullin-2/elongin B/elongin C)-like E3 enzymes that are also involved in
ubiquitination. In contrast to ubiquitin, the UBLs do not seem to form multi-UBL chains. UCRP(ISG15) resembles two ubiquitin moieties linked head-to-tail. Whether HUB1 functions as a modifier
is currently unclear. APG12 and URM1 are distinct from the other modifiers because they are unrelated in sequence to ubiquitin.
Data contributed by S. Jentsch, see references above. Rabbit Serum Hu, Ms, Rt E, IP, WB
0.1 ml / €440.00
  Acris Antibodies GmbH

CAND1 antibody

Western Blot analysis of CAND1 expression in transfected 293T cell line (H00055832-T01) by CAND1 MaxPab polyclonal antibody.

Lane 1: CAND1 transfected lysate(135.3 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

CAND1 antibody

CAND1 Hu, Ms, Rt
0.1 ml / €560.00
  Abnova Taiwan Corp.

CAND1 antibody

mouse testes; WB Suggested Anti-CAND1 Antibody . . Titration: 1 ug/ml. Positive Control: mouse testis; CAND1 antibody - middle region (ARP34584_P050) in mouse testes cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

CAND1 antibody

Human Brain; WB Suggested Anti-TIP120A Antibody Titration: 1.25ug/ml. ELISA Titer: 1:62500. Positive Control: Human brain; TIP120A antibody - N-terminal region (ARP34585_T100) in Human Brain cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Hu, Ms, Rt, Ze WB
0.1 mg / €310.00
  AVIVA Systems Biology

CAND1 antibody

Western Blot: TIP120A Antibody [NBP1-49918] - Detection of Human and Mouse CAND1 by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells.  Antibodies: Affinity purified rabbit anti-CAND1 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate.  CAND1 was also immunoprecipitated by rabbit anti-CAND1 antibody, which recognizes an upstream epitope.  For blotting immunoprecipitated CAND1 was used.  Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B). Rabbit Aff - Purified Hu, Ms IP, WB
0.1 mg / €440.00
  Novus Biologicals Inc.

CAND1 antibody

CAND1 Rabbit Purified Hu E, WB
0.1 ml / €510.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

CAND1

CAND1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

CAND1

CAND1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

CAND1 293T Cell Transient Overexpression Lysate(Denatured)

CAND1 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

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