CAPNS1 antibody

Principal name

CAPNS1 antibody

Alternative names for CAPNS1 antibody

Calpain small subunit 1, CSS1, CAPN4, CAPNS, Calcium-dependent protease small subunit 1, Calcium-dependent protease small subunit, Calpain regulatory subunit, Calcium-activated neutral proteinase small subunit

SwissProt ID

O88456 (Mouse), P04632 (Human), P13135 (Bovin), Q64537 (Rat)

Gene ID

826 (CAPNS1), 12336 (Capns1), 29156

Available reactivities

Hu (Human), Rt (Rat), Ms (Mouse), Can (Canine), GP (Guinea Pig)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Flow Cytometry (F)

Background of CAPNS1 antibody

Kit Component:
- KN302515G1, Capns1 gRNA vector 1 in pCas-Guide vector
- KN302515G2, Capns1 gRNA vector 2 in pCas-Guide vector
- KN302515D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:
CRISPR/CAS9 Genome Editing Manual

General readings

Miyake S., et al. (1987) Nucleic Acids Res. 14(22): 8805-8817.Ohno S., et al. (1986) Nucleic Acids Res. 14(13): 5559.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CAPNS1 antibody

  Anti-CAPNS1 Rabbit Polyclonal Antibody  
Predicted band size: 28 kDa . Positive control: Mouse spleen tissue, K562 and NIH/3T3 cell lysate. Recommended dilution: 1/200-1000. (Gel: 12%SDS-PAGE Lane1: Mouse spleen tissue lysate Lane2: K562 cell lysate Lane3: NIH/3T3 cell lysate Lysates: 60 ug per lane Primary antibody: 1/200 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 15 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CAPNS1 antibody

  Anti-CAPNS1 Rabbit Polyclonal Antibody  
Predicted band size: 28 kDa . Positive control: K562 and NIH/3T3 cell lysate. Recommended dilution: 1/500-2000. (Gel: 12%SDS-PAGE Lane1: K562 cell lysate Lane2: NIH/3T3 cell lysate Lysates: 60 ug per lane Primary antibody: 1/800 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 15 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CAPNS1 antibody

  Rabbit Polyclonal Anti-CAPNS1 Antibody - middle region  
WB Suggested Anti-CAPNS1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Human Lung Rabbit IgG Purified Can, GP WB
50 µg / €325.00
  OriGene Technologies, Inc.

CAPNS1 antibody

The recombinant human CAPNS1 (50ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CAPNS1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Lane 1.: CAPNS1 Recombinant protein
Lane 2.: HeLa cell lysate 
Lane 3.: A431 cell lysate 
Lane 4.: 293T cell lysate 
Lane 5.: Balb/3T3 cell lysate
Lane 6.: U87MG cell lysate Mouse IgG1 AT1D11 Purified Hu E, F, ICC/IF, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

CAPNS1 antibody

The recombinant human CAPNS1 (50ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CAPNS1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Lane 1.: CAPNS1 Recombinant protein
Lane 2.: HeLa cell lysate 
Lane 3.: A431 cell lysate 
Lane 4.: 293T cell lysate 
Lane 5.: Balb/3T3 cell lysate
Lane 6.: U87MG cell lysate Mouse IgG1 AT1D11 Purified Hu E, F, ICC/IF, WB
50 µl / €220.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

CAPNS1 - human gene knockout kit via CRISPR

CAPNS1 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

CAPNS1 - human gene knockout kit via CRISPR

CAPNS1 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

CAPNS1 - human gene knockout kit via CRISPR

CAPNS1 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

CAPNS1 - human gene knockout kit via CRISPR

CAPNS1 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Capns1 - mouse gene knockout kit via CRISPR

Capns1 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Capns1 - mouse gene knockout kit via CRISPR

Capns1 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Capns1 - mouse gene knockout kit via CRISPR

Capns1 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Capns1 - mouse gene knockout kit via CRISPR

Capns1 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

CAPNS1 (transcript variant 2)

  Recombinant protein of human calpain, small subunit 1 (CAPNS1), transcript variant 2  
CAPNS1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

CAPNS1 (transcript variant 1)

  Recombinant protein of human calpain, small subunit 1 (CAPNS1), transcript variant 1  
CAPNS1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

CAPNS1 (84-268, His-tag)

CAPNS1 Human Purified > 95 % by SDS - PAGE E. coli
0.5 mg / €730.00
  Acris Antibodies GmbH

CAPNS1 (84-268, His-tag)

CAPNS1 Human Purified > 95 % by SDS - PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, CAPNS1 (mGFP-tagged)-Human calpain, small subunit 1 (CAPNS1), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, CAPNS1 (mGFP-tagged)-Human calpain, small subunit 1 (CAPNS1), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €990.00
  OriGene Technologies, Inc.

Lenti ORF particles, CAPNS1 (mGFP-tagged)-Human calpain, small subunit 1 (CAPNS1), transcript variant 2, 200 uL, >10^7 TU/mL

Lenti ORF particles, CAPNS1 (mGFP-tagged)-Human calpain, small subunit 1 (CAPNS1), transcript variant 2, 200 uL, >10^7 TU/mL  
200 µl / €960.00
  OriGene Technologies, Inc.

Lenti ORF particles, CAPNS1 (Myc-DDK-tagged)-Human calpain, small subunit 1 (CAPNS1), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, CAPNS1 (Myc-DDK-tagged)-Human calpain, small subunit 1 (CAPNS1), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

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