Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), GP (Guinea Pig), Ye (Yeast)
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection))
Background of Carboxypeptidase A3 antibody
Carboxypeptidase A (CPA) is a pancreatic exopeptidase which hydrolyses the peptide bond adjacent to the C-terminal end in polypeptide chains. Mast cell carboxypeptidase A (MC-CPA), a part of the peptidase M14 family, is a highly conserved metalloprotease localized to the secretory granules, along with trytases and chymases. MC-CPA is stored as an active enzyme in the granule and is released, along with other inflammatory mediators, upon mast cell degranulation. MC-CPA mirrors pancreatic carboxypeptidase A in cleaving COOH-terminal aromatic and aliphatic amino acid residues. The optimum pH of MC-CPA is between neutral and basic, depending upon the substrate. The MC-CPA gene, CPA3, resides on chromosome 3 and contains 11 exons.
Western Blot: CPA3 Lysate [NBL1-09425] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for CPA3
Flow cytometric analysis of K562 cells using Carboxypeptidase A3 Antibody Cat.-No AP51045PU-N (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western Blot: CPA3 Antibody [NBP1-86117] Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 | Lane 2: Negative control (vector only transfected HEK293T lysate) | Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells.
Western Blot: CPA3 Antibody [NBP1-86130] Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 | Lane 2: Negative control (vector only transfected HEK293T lysate) | Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells.
Immunohistochemical staining of human small intestine shows strong cytoplasmic positivity in a subset of inflammatory cells in stroma.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.