Carboxypeptidase M antibody

Principal name

Carboxypeptidase M antibody

Alternative names for Carboxypeptidase M antibody

CPM

SwissProt ID

P14384 (Human), Q80V42 (Mouse)

Gene ID

1368 (CPM), 70574 (Cpm)

Available reactivities

Hu (Human)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Frozen Sections (C), Immunoprecipitation (IP), Flow Cytometry (F), Paraffin Sections (P)

Background of Carboxypeptidase M antibody

Monoclonal antibody (MoAb) M27 was generated after immunization of mice with the human B-lineage acute lymphoblastic leukemia cell line Pre-ALP. Under reducing conditions, MoAb M27 precipitated a 60- kD surface-membrane molecule from Pre-ALP cells. Expression cloning of Pre-ALP cDNA showed that M27 recognizes carboxypeptidase M (CPM), a cell-surface, zinc-dependent protease known to cleave off basic C-terminal amino acids from peptide hormones. Using M27 antibody, CPM was detected only at discrete B lymphocyte developmental stages, namely on committed precursors and on germinal center cells. CPM was also expressed on mature T cells, mainly after activation. These results provide the first description of a carboxy-peptidase on lymphoid cells. In addition, CPM was found on granulocytes and monocytes but not on their progenitors. CPM was found to be expressed with CD10 and CD13 respectively on lymphoid and myeloid cells. (de Saint Vis B et al., Blood 1995,86,1098-105).

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Carboxypeptidase M antibody

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-CPM mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 oC for 3 min, TA807285)(1:150) Mouse IgG1 OTI7C1 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Carboxypeptidase M antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-CPM mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA807289)(1:150) Mouse IgG1 OTI1F8 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Carboxypeptidase M antibody

Sample(30 ug whole cell lysate). A:Hep G2 . 7.5% SDS PAGE. TA308290 diluted at 1:500 Rabbit IgG Aff - Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Carboxypeptidase M antibody

Western blot analysis of extracts from A549 cells, using CPM antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Carboxypeptidase M antibody

Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-CPM mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA807283)(1:150) Mouse IgG1 OTI8E2 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Carboxypeptidase M antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-CPM mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 oC for 3 min, TA807284)(1:150) Mouse IgG2b OTI6C8 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Carboxypeptidase M antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CPM (RC224582, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CPM.(1:2000) Mouse IgG1 OTI1D8 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Carboxypeptidase M (Center) antibody

Western blot analysis of Carboxypeptidase M Antibody Cat.-No AP51052PU-N in 293 cell line lysates (35ug/lane).This demonstrates the CPM antibody detected the CPM protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Carboxypeptidase M antibody

Carboxypeptidase M Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

Carboxypeptidase M antibody

CPM MaxPab polyclonal antibody. Western Blot analysis of CPM expression in human placenta. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

Carboxypeptidase M antibody

Western blot analysis of extracts from A549 cells, using CPM Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, WB
50 µg / €205.00
  Assay Biotech

Carboxypeptidase M antibody

Western blot analysis of extracts from A549 cells, using CPM Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, WB
0.1 mg / €310.00
  Assay Biotech

Carboxypeptidase M antibody

FITC-M27 expression on leukaemia B lymphoid precursors Mouse IgG1 M27 Purified Hu C, F, IP
50 µg / €330.00
  Dendritics SAS

Carboxypeptidase M antibody

FITC-M27 expression on leukaemia B lymphoid precursors Mouse IgG1 M27 Purified Hu C, F, IP
0.1 mg / €440.00
  Dendritics SAS

CRISPR

Catalog No. Species Donor Vector  

CPM - human gene knockout kit via CRISPR

  CPM - human gene knockout kit via CRISPR  
CPM - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

CPM - human gene knockout kit via CRISPR

  CPM - human gene knockout kit via CRISPR  
CPM - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

CPM - human gene knockout kit via CRISPR

  CPM - human gene knockout kit via CRISPR  
CPM - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

CPM - human gene knockout kit via CRISPR

  CPM - human gene knockout kit via CRISPR  
CPM - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Carboxypeptidase M (transcript variant 3)

Carboxypeptidase M Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
10 µg / €139.00
  OriGene Technologies, Inc.

Carboxypeptidase M

Carboxypeptidase M Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

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