CARD5 / PYCARD / ASC antibody

Principal name

CARD5 / PYCARD / ASC antibody

Alternative names for CARD5 / PYCARD / ASC antibody

TMS1, PYD and CARD domain-containing protein, Target of methylation-induced silencing 1, Caspase recruitment domain-containing protein 5, poptosis-associated speck-like protein containing a CARD

SwissProt ID

Q8HXK9 (Bovin), Q9EPB4 (Mouse), Q9ULZ3 (Human)

Gene ID

29108 (PYCARD), 66824 (Pycard), 3829108 (map)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Por (Porcine)

Available hosts

Rabbit, Mouse, Goat

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunoprecipitation (IP), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F)

Background of CARD5 / PYCARD / ASC antibody

ASC (apoptosis-associated speck-like protein containing a CARD) or TMS 1 (target of methylation-induced silencing) is a recently identified CpG island-associated gene that becomes hypermethylated and silenced in cells overexpressing DNA cytosine-5-methyltransferase-1. TMS1 is aberrantly methylated and silenced in human breast cancer cells. Forty percent (11 of 27) of primary breast tumors exhibited aberrant methylation of ASC. Ectopic expression of ASC induced apoptosis in 293 cells and inhibited the survival of human breast cancer cells. The methylation-mediated silencing of ASC may confer a survival advantage by allowing cells to escape from apoptosis, supporting a new role for aberrant methylation in breast tumorigenesis. ASC encodes a 22-kDa predicted protein containing a COOH-terminal caspase recruitment domain (CARD). However, it is structurally unrelated to other known CARD adaptor and regulatory proteins. Ectopic expression of ASC alone was able to trigger apoptosis in 293 cells, and cell death correlated with relocalization of ASC from the cytoplasm to perinuclear, ball-like structures. Several lines of evidence support the idea that redistribution of ASC is an intermediate step in a ASC-triggered apoptotic pathway.

General readings

General / background references:Masumoto J et al. Murine ortholog of ASC, a CARD-containing protein, self-associates and exhibits restricted distribution in developing mouse embryos. Exp Cell Res 262:128-33 (2001). PubMed PMID: 11139337
Conway KE et al. TMS1, a novel proapoptotic caspase recruitment domain protein, is a target of methylation-induced gene silencing in human breast cancers. Cancer Res 60:6236-42 (2000). PubMed PMID: 11103776
McConnell BB & Vertino PM Activation of a caspase-9-mediated apoptotic pathway by subcellular redistribution of the novel caspase recruitment domain protein TMS1. Cancer Res 60:6243-7 (2000). PubMed PMID: 11103777
Masumoto J et al. ASC, a novel 22-kDa protein, aggregates during apoptosis of human promyelocytic leukemia HL-60 cells. J Biol Chem 274:33835-8 (1999). PubMed PMID: 10567338

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CARD5 / PYCARD / ASC antibody

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-PYCARD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800080) Mouse IgG1 OTI2A7 Purified Hu F, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CARD5 / PYCARD / ASC antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PYCARD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800081) Mouse IgG1 OTI3E9 Purified Hu F, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

CARD5 / PYCARD / ASC antibody

Western blot analysis of extracts of various cell lines, using PYCARD antibody. Rabbit IgG Purified Hu, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

CARD5 / PYCARD / ASC antibody

PYCARD MaxPab polyclonal antibody. Western Blot analysis of PYCARD expression in human spleen. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

CARD5 / PYCARD / ASC (C-term) antibody

TA302645 staining (1µg/ml) of U937 lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Hu E, P, WB
20 µg / €200.00
  Acris Antibodies GmbH

CARD5 / PYCARD / ASC antibody

Staining of Mouse (A) and Rat (B) Spleen lysates using PYCARD Antibody Cat.-No AP32825PU-N at 2 µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rt E, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

CARD5 / PYCARD / ASC (C-term) antibody

TA302645 staining (1µg/ml) of U937 lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CARD5 / PYCARD / ASC antibody

Western blot analysis of ASC in HL60 whole cell lysate in the absence (A) or presence (B) of blocking peptide (2287P) with ASC antibody at 1 ug /ml. Rabbit IgG Aff - Purified Hu E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CARD5 / PYCARD / ASC antibody

Predicted band size: 25 kDa . Positive control: HL60, A549, Raji and RAW264.7 cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lane1: HL60 cell lysate Lane2: A549 cell lysate Lane3: Raji cell lysate Lane4: RAW264.7 cell lysate Lysates: 40 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CARD5 / PYCARD / ASC antibody

Western blot analysis of extracts from RAW264.7 cells, using ASC antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CARD5 / PYCARD / ASC antibody

Western Blot: ASC/TMS1 Antibody [TA336600] - Western blot analysis of ASC in HL60 whole cell lysate (A) with anti-ASC using TA336600 at 1 ug /ml. Rabbit Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

CARD5 / PYCARD / ASC antibody

Immunohistochemistry: ASC/TMS1 Antibody [TA336850] - IHC of ASC/TMS1 in mouse intestine at a 1:400 dilution. Rabbit Purified Hu, Ms, Rt C, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CARD5 / PYCARD / ASC antibody

Immunohistochemistry: ASC/TMS1 Antibody [TA336851] - IHC analysis of ASC/TMS1 in mouse intestine using DAB with hematoxylin counterstain. Rabbit Purified Hu, Ms C, ICC/IF, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

CARD5 / PYCARD / ASC antibody

Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-PYCARD monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human). Mouse IgG1 OTI1A2 Purified Hu F, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CARD5 / PYCARD / ASC antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PYCARD (RC215592, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PYCARD. Mouse IgG1 OTI1H6 Purified Hu F, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CARD5 / PYCARD / ASC antibody

Western blot analysis of ASC expression in U937 (1), HL-60 (2), Jurkat(3), WR19L(4) and PC12 (5) using AM26452AF-N. Mouse IgG1 23-4 Azide Free Hu IP, WB
0.1 mg / €380.00
  Acris Antibodies GmbH

CARD5 / PYCARD / ASC antibody

Western blot analysis of ASC Antibody (Cat.-No AP06792PU-N) at 1/500 dilution in: Lane 1: DLD wohle cell lysate. Lane 2: HEK293T wohle cell lysate. Lane 3: Mouse brain Tissue lysate.Lane 4: Rat brain Tissue lysate. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

CARD5 / PYCARD / ASC antibody

AP07343PU-N PYCARD antibody staining of Formalin-Fixed Paraffin-Embedded Human Tonsil at 5 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, Alkaline Phosphatase-Streptavidin and Chromogen. Rabbit Aff - Purified Hu ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

CARD5 / PYCARD / ASC (31-46) antibody

Human Thymus: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit IgG Hu P, WB
50 µg / €470.00
  Acris Antibodies GmbH

CARD5 / PYCARD / ASC Control Peptide

CARD5 / PYCARD / ASC
50 µg / €160.00
  Acris Antibodies GmbH

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