Cardiac Troponin I antibody

Principal name

Cardiac Troponin I antibody

Alternative names for Cardiac Troponin I antibody

TNNI3, TNNC1

SwissProt ID

P02646 (Rabit), P08057 (Bovin), P19429 (Human), P23693 (Rat), P27673 (Chick), P48787 (Mouse), Q5PYI0 (Horse), Q863B6 (Felca), Q8MKD5 (Canfa)

Gene ID

7137 (TNNI3), 511094, 403566, 21954 (Tnni3), 29248

Available reactivities

Bov (Bovine), Can (Canine), Fe (Feline), Gt (Goat), Hu (Human), Por (Porcine), Rb (Rabbit), Fi (Fish), Mam (Mammalia), Amph (Amphibian), Chk (Chicken), Ms (Mouse), Rt (Rat), Frog, Prim (Primate), Xen (Xenopus), Ze (Zebrafish), Mky (Monkey)

Available hosts

Mouse, Goat, Rabbit

Available applications

Frozen Sections (C), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Western blot / Immunoblot (WB), ELISA (capture) (E(capture)), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Lateral Flow (LF), Luminex (LMNX)

Background of Cardiac Troponin I antibody

Troponin is the inhibitory or contractile regulating protein complex of striated muscle. It is located periodically along the thin filament of the muscle and consists of three distinct proteins: troponin I, troponin C, and troponin T. Likewise, the troponin I subunit exists in three separate isoforms; two in fast-twitch and slow-twitch skeletal muscle fibers, and one in cardiac muscle. The cardiac isoform (cTnI) is about 40% dissimilar, has a molecular weight of 22,500 daltons, Cardiac troponin I (cTnI) has been useful in the differential diagnosis of patients presenting to Emergency Departments (ED) with chest pain 18-20. Myocardial infarction is diagnosed when blood levels of sensitive and specific biomarkers, such as cardiac troponin, the MB isoenzyme of creatine kinase (CK-MB), and myoglobin, are increased in a clinical setting of acute ischemia. The most recently described and preferred biomarker for myocardial damage is cardiac troponin (I or T). The cardiac troponins exhibit myocardial tissue specificity and high sensitivity. The level of cTnI remains elevated for a much longer period of time (6-10 days), thus providing for a longer window of detection of cardiac injury. Normal levels of cTn I in the blood are very low. After the onset of an AMI, cTnI levels increase substantially and are measurable in serum within 4 to 6 hours, with peak concentrations reached in approximately 12 to 24 hours after infarction. The cTnI Enzyme Immunoassay provides a rapid, sensitive, and reliable assay for the quantitative measurement of cardiac-specific troponin I. The antibodies developed for the test will determine a minimal concentration of 1.0 ng/ml, and theres no cross-reactivity with human cardiac or skeletal troponin T or I.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Cardiac Troponin I (+ skeletal) antibody

Cardiac Troponin I Mouse IgG2b C5 Purified Amph, Bov, Chk, Fi, Hu, Ms, Rb E, WB
1 mg / €560.00
  Acris Antibodies GmbH

Cardiac Troponin I antibody

Immunofluorescent staining of A549 cells using anti-TNNI3 mouse monoclonal antibody (TA500057). Mouse IgG1 OTI4E5 Purified Can, Hu, Mky ICC/IF, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

Cardiac Troponin I antibody

TNNI3 ELISA with 4E5 Capture (TA600516) and TPC110 Detection (TA700515) Antibodies. Substrate used: Recombinant Human TNNI3 (TP314740) Mouse IgG1 OTITPC110 Purified Hu E, LMNX
50 µg / €345.00
  OriGene Technologies, Inc.

Cardiac Troponin I antibody

Staining of Human Heart lysate using TNNI3 Antibody Cat.-No AP32345PU-N at 1 μg/ml (35μg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu E, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Cardiac Troponin I antibody

Predicted band size: 24kDa. Positive control: Mouse heart tissue lysate. Recommended dilution: 1/200-1000. (Gel: 12%SDS-PAGE Lysate: 40 ug Primary antibody: 1/200 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/8000 dilution Exposure time: 10 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Cardiac Troponin I antibody

Western blot analysis of TNNI3 expression in Mouse hearttissue lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Cardiac Troponin I antibody

Western blot analysis of TNNI3 expression in Jurkat whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Cardiac Troponin I antibody

Western Blot: Troponin I Type 3 (cardiac) Antibody (2C9) [TA337220] - Western blot analysis of clone 2C9 in human heart at 0.5 and 1.0 ug/ml. Mouse IgG2b 2C9 Purified Hu, Ms C, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Cardiac Troponin I antibody

Western Blot: Troponin I Type 3 (cardiac) Antibody (1E10) [TA337221] - Western blot analysis of clone 1E10 in human heart at 0.5 and 1.0 ug/ml. Mouse IgG2b 1E10 Purified Hu, Ms C, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Cardiac Troponin I (117-127) antibody

TA348990 (0.2 ug/ml) staining of Human Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Cardiac Troponin I antibody

Immunohistochemical staining of paraffin-embedded prostate tissue within the normal limits using anti-TNNI3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500255, Dilution 1:50) Mouse IgG2a OTI3G2 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Cardiac Troponin I antibody

Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-TNNI3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500256, Dilution 1:50) Mouse IgG1 OTI4A3 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Cardiac Troponin I antibody

TNNI3 ELISA with 4A3 Capture (TA600515) and TPC110 Detection (TA700515) Antibodies. Substrate used: Recombinant Human TNNI3 (TP314740) Mouse IgG1 OTI4A3 Purified Hu E, LMNX
0.1 mg / €325.00
  OriGene Technologies, Inc.

Cardiac Troponin I antibody

TNNI3 ELISA with 4E5 Capture (TA600516) and TPC110 Detection (TA700515) Antibodies. Substrate used: Recombinant Human TNNI3 (TP314740) Mouse IgG1 OTI4E5 Purified Hu E, LMNX
0.1 mg / €325.00
  OriGene Technologies, Inc.

Cardiac Troponin I antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TNNI3 (RC214740, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TNNI3. Mouse IgG2a OTI13E9 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Cardiac Troponin I antibody

Immunohistochemical staining of paraffin-embedded Human muscle tissue using anti-TNNI3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807911) Mouse IgG1 OTI3H9 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Cardiac Troponin I antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TNNI3 (RC214740, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TNNI3. Mouse IgG2b OTI3B4 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Cardiac Troponin I antibody

Immunohistochemical staining of paraffin-embedded Human adult heart tissue using anti-TNNI3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807946)(1:150) Mouse IgG1 OTI8G8 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Cardiac Troponin I antibody

Immunohistochemical staining of paraffin-embedded Human muscle tissue using anti-TNNI3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA808020) Mouse IgG1 OTI14A2 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Cardiac Troponin I antibody

Immunohistochemical staining of paraffin-embedded Human adult heart tissue using anti-TNNI3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA808038)(1:150) Mouse IgG1 OTI4C1 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

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