Cardiac Troponin I antibody

Principal name

Cardiac Troponin I antibody

Alternative names for Cardiac Troponin I antibody

TNNI3, TNNC1

SwissProt ID

P02646 (Rabit), P08057 (Bovin), P19429 (Human), P23693 (Rat), P27673 (Chick), P48787 (Mouse), Q5PYI0 (Horse), Q863B6 (Felca), Q8MKD5 (Canfa)

Gene ID

7137 (TNNI3), 511094, 403566, 21954 (Tnni3), 29248, 7138 (TNNT1)

Available reactivities

Bov (Bovine), Can (Canine), Fe (Feline), Gt (Goat), Hu (Human), Por (Porcine), Rb (Rabbit), Fi (Fish), Mam (Mammalia), Amph (Amphibian), Chk (Chicken), Ms (Mouse), Rt (Rat), Frog, Mky (Monkey), Prim (Primate), Xen (Xenopus), Ze (Zebrafish)

Available hosts

Mouse, Goat, Rabbit

Available applications

Frozen Sections (C), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Western blot / Immunoblot (WB), ELISA (capture) (E(capture)), Paraffin Sections (P), Radioimmunoassay (R), Immunocytochemistry/Immunofluorescence (ICC/IF), Lateral Flow (LF), Luminex (LMNX)

Background of Cardiac Troponin I antibody

Troponin I (cTnI) is 1 of 3 subunits, along with troponin C (TnC) and troponin T (TnT) of troponin complex found in cardiac muscle. cTnI binds to actin in thin myofilaments to hold the troponin-tropomyosin complex in place. Phosphorylation of cardiac isoform of TnI at serines 22,23 in the unique amino-terminal end molecule decreases the ca lcium sensitivity of the sarcomere, promotes calcium dissociation from troponin C and by ext ension enhances rates of cross-bridge cycling and diastolic relaxation (Noland, Jr. et al., 1995; Noland et al., 1989). In addition, studies using reconstituted fibers and mutational analysis have shown that PKC phosp horylation of TnI (largely at Ser43) inhibits the actin-cross bridge reaction and reduces the Ca ++ dependent actomyosin ATPase rate as well as the calcium sensitivity of force generation (Noland, Jr. and Kuo, 1991). Phosphorylation at Thr144 (mediated by several PKC isoforms) reduces maximal tension development and cross-bridge cycling rates (Sumandea et al., 2008). Importantly, changes in th e phosphorylation at each of these sites have been shown to be stage-specific with regard to cardiac disease progression (Walker et al., 2010).

General readings

General / background references:Sarko J & Pollack CV Cardiac troponins. J Emerg Med 23:57-65 (2002). PubMed PMID: 12217473
Gomes AV et al. The role of troponins in muscle contraction. IUBMB Life 54:323-33 (2002). PubMed PMID: 12665242
Hamm CW Acute coronary syndromes. The diagnostic role of troponins. Thromb Res 103 Suppl 1:S63-9 (2001). PubMed PMID: 11567671
Labugger R et al. Extensive troponin I and T modification detected in serum from patients with acute myocardial infarction. Circulation 102:1221-6 (2000). PubMed PMID: 10982534
Wu AH et al. Characterization of cardiac troponin subunit release into serum after acute myocardial infarction and comparison of assays for troponin T and I. American Association for Clinical Chemistry Subcommittee on cTnI Standardization. Clin Chem 44:1198-208 (1998). PubMed PMID: 9625043
Katrukha AG et al. Troponin I is released in bloodstream of patients with acute myocardial infarction not in free form but as complex. Clin Chem 43:1379-85 (1997). PubMed PMID: 9267317
Jaquet K et al. Pattern formation on cardiac troponin I by consecutive phosphorylation and dephosphorylation. Eur J Biochem 231:486-90 (1995). PubMed PMID: 7635159
Cummins B et al. Cardiac-specific troponin-I radioimmunoassay in the diagnosis of acute myocardial infarction. Am Heart J 113:1333-44 (1987). PubMed PMID: 3591601

  1. Quick-Filters:
  2. Cited (1)
  3. Highly Validated (3)

Items 1 to 20 of 181 total

per page
Page:
  1. 1
  2. 2
  3. 3
  4. 4
  5. 5

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Cardiac Troponin I (+ skeletal) antibody

Cardiac Troponin I Mouse IgG2b C5 Purified Amph, Bov, Chk, Fi, Hu, Ms, Rb E, WB
1 mg / €540.00
  Acris Antibodies GmbH

Cardiac Troponin I antibody

Immunofluorescent staining of A549 cells using anti-TNNI3 mouse monoclonal antibody (TA500057). Mouse IgG1 OTI4E5 Purified Can, Hu, Mky ICC/IF, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

Cardiac Troponin I antibody

TNNI3 ELISA with 4E5 Capture (TA600516) and TPC110 Detection (TA700515) Antibodies. Substrate used: Recombinant Human TNNI3 (TP314740) Mouse IgG1 OTITPC110 Purified Hu E, LMNX
50 µg / €345.00
  OriGene Technologies, Inc.

Cardiac Troponin I (cardiac) antibody

Western Blot: Troponin I (cardiac) Antibody (4E5) [NBP1-48026] - Western blot analysis of 35ug of cell extracts from Rat (PC12) cells using anti-TNNI3 antibody. Mouse 4E5 Aff - Purified Can, Hu, Mky ICC/IF, WB
0.1 ml / €620.00
  Novus Biologicals Inc.

Cardiac Troponin I antibody

Staining of Human Heart lysate using TNNI3 Antibody Cat.-No AP32345PU-N at 1 μg/ml (35μg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu E, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

Cardiac Troponin I antibody

Predicted band size: 24kDa. Positive control: Mouse heart tissue lysate. Recommended dilution: 1/200-1000. (Gel: 12%SDS-PAGE Lysate: 40 ug Primary antibody: 1/200 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/8000 dilution Exposure time: 10 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Cardiac Troponin I antibody

Western blot analysis of TNNI3 expression in Mouse hearttissue lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Cardiac Troponin I antibody

Western blot analysis of TNNI3 expression in Jurkat whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Cardiac Troponin I antibody

Western Blot: Troponin I Type 3 (cardiac) Antibody (2C9) [TA337220] - Western blot analysis of clone 2C9 in human heart at 0.5 and 1.0 ug/ml. Mouse IgG2b 2C9 Purified Hu, Ms C, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Cardiac Troponin I antibody

Western Blot: Troponin I Type 3 (cardiac) Antibody (1E10) [TA337221] - Western blot analysis of clone 1E10 in human heart at 0.5 and 1.0 ug/ml. Mouse IgG2b 1E10 Purified Hu, Ms C, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Cardiac Troponin I (117-127) antibody

TA348990 (0.2 ug/ml) staining of Human Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Cardiac Troponin I antibody

Immunohistochemical staining of paraffin-embedded prostate tissue within the normal limits using anti-TNNI3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500255, Dilution 1:50) Mouse IgG2a OTI3G2 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Cardiac Troponin I antibody

Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-TNNI3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500256, Dilution 1:50) Mouse IgG1 OTI4A3 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Cardiac Troponin I antibody

TNNI3 ELISA with 4A3 Capture (TA600515) and TPC110 Detection (TA700515) Antibodies. Substrate used: Recombinant Human TNNI3 (TP314740) Mouse IgG1 OTI4A3 Purified Hu E, LMNX
0.1 mg / €325.00
  OriGene Technologies, Inc.

Cardiac Troponin I antibody

TNNI3 ELISA with 4E5 Capture (TA600516) and TPC110 Detection (TA700515) Antibodies. Substrate used: Recombinant Human TNNI3 (TP314740) Mouse IgG1 OTI4E5 Purified Hu E, LMNX
0.1 mg / €325.00
  OriGene Technologies, Inc.

Cardiac Troponin I antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TNNI3 (RC214740, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TNNI3. Mouse IgG2a OTI13E9 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Cardiac Troponin I antibody

Immunohistochemical staining of paraffin-embedded Human muscle tissue using anti-TNNI3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807911) Mouse IgG1 OTI3H9 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Cardiac Troponin I antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TNNI3 (RC214740, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TNNI3. Mouse IgG2b OTI3B4 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Cardiac Troponin I antibody

Immunohistochemical staining of paraffin-embedded Human adult heart tissue using anti-TNNI3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807946)(1:150) Mouse IgG1 OTI8G8 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Cardiac Troponin I antibody

Immunohistochemical staining of paraffin-embedded Human muscle tissue using anti-TNNI3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA808020) Mouse IgG1 OTI14A2 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Items 1 to 20 of 181 total

per page
Page:
  1. 1
  2. 2
  3. 3
  4. 4
  5. 5
  • LinkedIn