CARS antibody

Principal name

CARS antibody

Alternative names for CARS antibody

Cysteinyl-tRNA synthetase, Cysteine-tRNA ligase, CysRS, Cysteinyl-tRNA synthetase cytoplasmic

SwissProt ID

P49589 (Human), Q5F408 (Chick), Q9ER72 (Mouse)

Gene ID

833 (CARS), 833836 (GRF3)

Available reactivities

Hu (Human), Chk (Chicken), Ms (Mouse), Rt (Rat), African clawed frog, Bov (Bovine), Dros (Drosophila), Can (Canine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit), Ze (Zebrafish), Por (Porcine), Ye (Yeast)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of CARS antibody

CARS is a class 1 aminoacyl-tRNA synthetase, cysteinyl-tRNA synthetase. Each of the twenty aminoacyl-tRNA synthetases catalyzes the aminoacylation of a specific tRNA or tRNA isoaccepting family with the cognate amino acid. This gene is one of several located near the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer. Western blots using two different antibodies against two unique regions of this protein target confirm the same apparent molecular weight in our tests.This gene encodes a class 1 aminoacyl-tRNA synthetase, cysteinyl-tRNA synthetase. Each of the twenty aminoacyl-tRNA synthetases catalyzes the aminoacylation of a specific tRNA or tRNA isoaccepting family with the cognate amino acid. This gene is one of several located near the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms.

General readings

Liu,C., (2007) J. Mol. Biol. 367 (4), 1063-1078

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CARS antibody

Immunofluorescence of purified MaxPab antibody to CARS on HeLa cell. [antibody concentration 7 ug/ml] Mouse Purified Hu E(detection), ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

CARS antibody

Host: Rabbit; Target Name: CARS; Sample Tissue: Jurkat Whole cell lysates; Antibody Dilution: 1.0 ug/ml.CARS is supported by BioGPS gene expression data to be expressed in Jurkat Rabbit IgG Purified Can, Eq, GP, Hu, Ms, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

CARS antibody

WB Suggested Anti-CARS Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: MCF7 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

CARS antibody

WB Suggested Anti-CARS Antibody Titration: 0.2-1 ug/ml; Positive Control: HepG2 cell lysate Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Por, Rb, Rt, Ye, Ze P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

CARS antibody

WB Suggested Anti-CARS Antibody Titration: 0.2-1 ug/ml; Positive Control: HepG2 cell lysate Rabbit IgG Purified Hu, Rt P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

CARS antibody

WB Suggested Anti-CARS Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: THP-1 cell lysate Rabbit IgG Purified Hu, Ye WB
50 µg / €325.00
  OriGene Technologies, Inc.

CARS antibody

CARS Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

CARS antibody

Human MCF-7; WB Suggested Anti-CARS Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: MCF7 cell lysate; CARS antibody - middle region (ARP40294_P050) in Human MCF-7 cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Chk, Dros, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

CARS antibody

Human Lung; Rabbit Anti-CARS Antibody. Catalog Number: ARP40295. Paraffin Embedded Tissue: Human Lung. Cellular Data: Alveolar cells. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X; CARS antibody - C-terminal region (ARP40295_P050) in Human Lung cells using Immunohistochemistry Rabbit Aff - Purified African clawed frog, Chk, Hu, Ms, Rt P, WB
50 µg / €350.00
  AVIVA Systems Biology

CARS antibody

Human Lung; Rabbit Anti-CARS Antibody. Catalog Number: ARP40297. Paraffin Embedded Tissue: Human Lung. Cellular Data: Alveolar cells. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X; CARS antibody - N-terminal region (ARP40297_P050) in Human Lung cells using Immunohistochemistry Rabbit Aff - Purified Hu P, WB
50 µg / €350.00
  AVIVA Systems Biology

CARS antibody

Human THP-1; WB Suggested Anti-CARS Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: THP-1 cell lysate; CARS antibody - N-terminal region (ARP40298_P050) in Human THP-1 cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

CARS antibody

Immunohistochemistry-Paraffin: CARS Antibody [NBP1-86623] - Immunohistochemical staining of human pancreas shows cytoplasmic positivity in exocrine glandular cells. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

CARS antibody

Immunohistochemistry-Paraffin: CARS Antibody [NBP1-86624] - Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells. Rabbit IgG Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

CARS (transcript variant 3)

CARS Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

CARS (transcript variant 1)

CARS Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

CARS

CARS Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

CARS

CARS Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

CARS

CARS Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

CARS

CARS Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

CARS 293T Cell Transient Overexpression Lysate(Denatured)

CARS 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

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