CAS9 antibody

Principal name

CAS9 antibody

Alternative names for CAS9 antibody

csn1, SpyCas9, CRISPR-associated endonuclease Cas9/Csn1

SwissProt ID

Q99ZW2

Gene ID

901176 (SPy_1046)

Available reactivities

S. pyrogenes (Streptococcus pyrogenes)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF)

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  2. Highly Validated (1)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CAS9 antibody

Immunoprecipitation of WT Cas9 and Cas9 nickase by using TA190309. HEK293T were transfected with either WT CAS9 plasmid (GE100002), CAS9 nickase plasmid (GE100019), or negative control vector (PS100001). Input: 10ug total cell lysates; WB was performed using TA190309 (1:2000). IP: 100ug of cell lysates were precipitated with 20ug of TA190309 or control antibody, and 5ug Protein G Beads. Mouse anti-DDK monoclonal antibody (TA50011) was used in WB to detect the immuno-precipitated Cas9 (as Cas9 is Myc-DDK tagged). Rabbit Purified S. pyrogenes WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CAS9 antibody

Western blot was performed on protein extracts from HEK293 cells transfected with a myc-tagged Cas9 using the CRISPR/Cas9 antibody as well as two commercially available purified monoclonal myc tag antibodies (supplier A and B). The three antibodies were used at different dilutions (antibody stocks: purified monoclonal Myc A 1ug/ul, Myc B 1ug/ul and Cas9 1.9 ug/ul). The ladder has been revealed by using the HRP coupled Blue ladder antibody. Exposure time was 1 hour. Mouse IgG1 7A9 Purified S. pyrogenes ICC/IF, IP, WB
50 µg / €475.00
  OriGene Technologies, Inc.

CAS9 antibody

Western blot was performed on protein extracts from HeLa cells transfected with a FLAG-tagged Cas9 using the Cas9 antibody. The antibody was used at different dilutions. The marker is shown on the left, the position of the FLAG-tagged Cas9 protein is indicated on the right. Mouse IgG1 7A9 HRP S. pyrogenes WB
0.5 ml / €495.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

CAS9 (C-term Myc/DDK tag)

CAS9 > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

CAS9 (N-term HIS tag)

CAS9 > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €299.00
  OriGene Technologies, Inc.

5 Item(s)

per page
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