Casein kinase I epsilon antibody

Principal name

Casein kinase I epsilon antibody

Alternative names for Casein kinase I epsilon antibody

CKI-epsilon, CKIe, CSNK1E

SwissProt ID

P49674 (Human), Q5ZLL1 (Chick), Q9JMK2 (Mouse)

Gene ID

1454 (CSNK1E), 3614547 (IPR007113)

Available reactivities

Can (Canine), Hu (Human), Ms (Mouse), Rt (Rat), Dros (Drosophila), African clawed frog, Bov (Bovine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit), Sh (Sheep), Ye (Yeast), Ze (Zebrafish)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of Casein kinase I epsilon antibody

Casein kises are operatiolly defined by their preferential utilization of acidic proteins such as caseins as substrates. CSNK1E can phosphorylate a large number of proteins. CSNK1E participates in Wnt sigling and is the central component of the circadian clock. It may act as a negative regulator of circadian rhythmicity by phosphorylating PER1 and PER2. CSNK1E inhibits cytokine-induced granuloytic differentiation.The protein encoded by this gene is a serine/threonine protein kise and a member of the casein kise I protein family, whose members have been implicated in the control of cytoplasmic and nuclear processes, including D replication and repair. The encoded protein is found in the cytoplasm as a monomer and can phosphorylate a variety of proteins, including itself. This protein has been shown to phosphorylate period, a circadian rhythm protein. Two transcript variants encoding the same protein have been found for this gene.

General readings

Hammerlein A, Weiske J, Huber O.
A second protein kinase CK1-mediated step negatively regulates Wnt signalling by disrupting the lymphocyte enhancer factor-1/beta-catenin complex.
Cell Mol Life Sci. 2005 Mar;62(5):606-18.
PMID: 15747065

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Casein kinase I epsilon antibody

Western blot analysis of extracts from LOVO cells, using CKI-e antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Casein kinase I epsilon antibody

Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-CSNK1E mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA803799) Mouse IgG1 OTI5D4 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Casein kinase I epsilon (C-term) antibody

TA302458 (1µg/ml) staining of HEK293 cell lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Casein kinase I epsilon antibody

WB Suggested Anti-CSNK1E Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: THP-1 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Sh, Ye, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

Casein kinase I epsilon antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CSNK1E (RC202436, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CSNK1E. Mouse IgG1 OTI2B3 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Casein kinase I epsilon antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CSNK1E (RC202436, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CSNK1E. Mouse IgG2a OTI5C9 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Casein kinase I epsilon antibody

Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-CSNK1E mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA803801) Mouse IgG2a OTI5H10 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Casein kinase I epsilon (incl. pos. control) antibody

Detection of endogenous Casein Kinase 1 epsilon: Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab CK1e-4D7(0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec) lane 1: HeLa; lane 2: HepG2; lane 3: HEK293; lane 4: SH-SY5Y; lane 5: MDCK; lane 6: PC12; lane 7: CMT 93; lane 8: Neuro 2A; lane 9: NIH 3T3. Mouse IgG1 4D7 Purified Can, Hu, Ms, Rt E, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

Casein kinase I epsilon (403-416) antibody

AP08800PU-N at 5 µg/ml, staining CSNK1E in Tonsil tissue by Immunohistochemistry (Formalin-Fixed Paraffin-Embedded). Goat IgG Aff - Purified Bakers Yeast, Can, Chk, Elephant, Eq, Hu, Mky, Ms, Platypus, Por, Prim, Rt, Tr, Xen E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

Casein kinase I epsilon (C-term) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Casein kinase I epsilon antibody

Immunohistochemistry analyzes of Casein Kinase Iε antibody (Cat.-No.: AP20419PU-N) in paraffin-embedded human placenta tissue. Rabbit Aff - Purified Hu, Ms ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Casein kinase I epsilon antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between TP53 and CSNK1E. HeLa cells were stained with anti-TP53 rabbit purified polyclonal 1:1200 and anti-CSNK1E mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Mouse IgG2a 2E1 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

Casein kinase I epsilon antibody

Immunoblot analysis of cell lysates.
Lane 1 : HeLa cell lysate.
 Lnae 2 : Ramos cell lysate.
Lane 3 : A-431 cell lysate.
Lane 4 : 293T cell lysate.
Lane 5 : Jurkat cell lysate.
Lane 6 : PC-12 cell lysate. Mouse AF6C1 Hu, Ms, Rt E, IP, WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

Casein kinase I epsilon antibody

Immunoprecipitation analysis of Jurkat lysates.
Lane 1 : Input.
Lane 2 : Precipitatates. Mouse AF26H1 Hu E, IP
0.1 ml / €390.00
  Abnova Taiwan Corp.

Casein kinase I epsilon antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with CSNK1E polyclonal antibody ( Cat # PAB2877 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma. Rabbit Hu E, P, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

Casein kinase I epsilon antibody

293 overexpressing CSNK1E and probed with CSNK1E polyclonal antibody ( Cat # PAB7264 ) ( mock transfection in first lane), tested by Origene. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

Casein kinase I epsilon antibody

Immunohistochemistry analysis of paraffin-embedded human placenta tissue, using CKI-epsilon Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, ICC/IF, P, WB
50 µg / €205.00
  Assay Biotech

Casein kinase I epsilon antibody

Immunohistochemistry analysis of paraffin-embedded human placenta tissue, using CKI-epsilon Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, ICC/IF, P, WB
0.1 mg / €310.00
  Assay Biotech

Casein kinase I epsilon antibody

Human THP-1; WB Suggested Anti-CSNK1E Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: THP-1 cell lysate; CSNK1E antibody - N-terminal region (ARP51987_P050) in Human THP-1 cells using Western Blot Rabbit Aff - Purified African clawed frog, Can, Dros, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

Casein kinase I epsilon antibody

Immunohistochemical analysis of CK1 epsilon staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

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