Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Chk (Chicken), Rb (Rabbit), Sh (Sheep), Ze (Zebrafish), Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine)
Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection))
Background of Casein kinase I isoform alpha-like antibody
Casein kinase I (also designated CKI) and casein kinase II (CKII) compose a family of serine/threonine protein kinases which are present in all eukaryotes examined to date. Casein kinase I family members, which include casein kinase I Alpha, I Gamma, I Delta and I Epsilon, have been implicated in the control of cytoplasmic and nuclear processes, including DNA replication and repair, membrane trafficking, circadian rhythm, cell cycle progression, chromosome segregation, apoptosis and cellular differentiation. Casein kinase I isoform alpha-like (CSNK1A1L) is a 337 amino acid protein that shares a high degree of sequence similarity with the alpha isoform of casein kinase 1. CSNK1A1L resides in the cytoplasm and participates in the Wnt signaling pathway. By utilizing ATP within its protein kinase domain, CSNK1A1L phosphorylates a large number of proteins.
Western Blot: CSNK1A1L Lysate [NBL1-09528] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for CSNK1A1L.
Human 721_B; WB Suggested Anti-CSNK1A1L Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: 721_B cell lysate; CSNK1A1L antibody - middle region (ARP52809_P050) in Human 721_B cells using Western Blot
Casein Kinase 1 alpha 1L antibody detects CSNK1A1L protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: CSNK1A1L protein stained by Casein Kinase 1 alpha 1L antibody (GTX105584) diluted at 1:500.Blue: Hoechst 33342 staining.
Immunohistochemical analysis of CK1 alpha staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of CK1 alpha staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).