Casein kinase II subunit alpha antibody

Principal name

Casein kinase II subunit alpha antibody

Alternative names for Casein kinase II subunit alpha antibody

CSNK2A1, CK2A1, CK II alpha

SwissProt ID

P19139 (Rat), P21868 (Chick), P28020 (Xenla), P33674 (Rabit), P68399 (Bovin), P68400 (Human), Q60737 (Mouse)

Gene ID

1457 (CSNK2A1), 1459 (CSNK2A2), 1460 (CSNK2B), 116549 (Csnk2a1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Chk (Chicken), Rb (Rabbit), Xen (Xenopus), Ce (C. elegans), Dros (Drosophila), Ze (Zebrafish), African clawed frog, Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Ye (Yeast)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Immunoprecipitation (IP), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), PLA/Proximity ligation assay (PLA), Flow Cytometry (F)

Background of Casein kinase II subunit alpha antibody

Catalytic subunit of a constitutively active serine/threonine-protein kise complex that phosphorylates a large number of substrates containing acidic residues C-termil to the phosphorylated serine or threonine. Regulates numerous cellular processes, such as cell cycle progression, apoptosis and transcription, as well as viral infection. May act as a regulatory node which integrates and coordites numerous sigls leading to an appropriate cellular response. During mitosis, functions as a component of the p53/TP53-dependent spindle assembly checkpoint (SAC) that maintains cyclin-B-CDK1 activity and G2 arrest in response to spindle damage. Also required for p53/TP53-mediated apoptosis, phosphorylating 'Ser-392' of p53/TP53 following UV irradiation. Can also negatively regulate apoptosis. Phosphorylates the caspases CASP9 and CASP2 and the apoptotic regulator NOL3. Phosphorylation protects CASP9 from cleavage and activation by CASP8, and inhibits the dimerization of CASP2 and activation of CASP8. Regulates transcription by direct phosphorylation of R polymerases I, II, III and IV. Also phosphorylates and regulates numerous transcription factors including NF-kappa-B, STAT1, CREB1, IRF1, IRF2, ATF1, SRF, MAX, JUN, FOS, MYC and MYB. Phosphorylates Hsp90 and its co-chaperones FKBP4 and CDC37, which is essential for chaperone function. Regulates Wnt sigling by phosphorylating CTNNB1 and the transcription factor LEF1. Acts as an ectokise that phosphorylates several extracellular proteins.

General readings

1. Keller D.M., Zeng X., et al., Mol. Cell 7:283-292(2001)
Keller D.M., Lu H. et al., J. Biol. Chem. 277:50206-50213(2002).
2. Trembley J.H., Tatsumi S., et al., Mol. Cell. Biol. 25:1446-1457(2005).
3. Niefind K., Guerra B., et al., Acta Crystallogr. D 56:1680-1684(2000).

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Casein kinase II subunit alpha antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between TP53 and CSNK2A1 HeLa cells were stained with anti-TP53 rabbit purified polyclonal 1:1200 and anti-CSNK2A1 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Mouse IgG2a 3D9 Purified Hu E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Casein kinase II subunit alpha antibody

Predicted band size: 45 kDa . Positive control: Mouse heart tissue, Hela and Jurkat cell lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lane1: Mouse heart tissue lysate Lane2: Hela cell lysate Lane3: Jurkat cell lysate Lysates: 40 ug per lane Primary antibody: 1/250 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 5 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Casein kinase II subunit alpha antibody

Gel: 8%SDS-PAGE, Lysate: 40 µg, Lane 1-4: K562 cells, 293T cells, Jurkat cells, mouse brain tissue, Primary antibody: CSNK2A1 Antibody at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 seconds Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Casein kinase II subunit alpha antibody

The anti-CSNK2A1 Pab (Cat. #TA325032) is used in Western blot to detect CSNK2A1 in mouse brain tissue lysate. Rabbit Ig Purified Bov, Chk, Hu, Ms, Rb, Rt, Xen P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

Casein kinase II subunit alpha antibody

Rat Csnk2a1 Antibody (N-term) (Cat. #TA328199) western blot analysis in 293 cell line , rat brain and cerebellum tissue lysates (35ug/lane).This demonstrates the Rat Csnk2a1 antibody detected the Rat Csnk2a1 protein (arrow). Rabbit Purified Bov, Chk, Dros, Hu, Ms, Rb, Rt, Xen WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

Casein kinase II subunit alpha antibody

WB Suggested Anti-CSNK2A1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: 721_B cell lysateCSNK2A1 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ye, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

Casein kinase II subunit alpha antibody

Immunohistochemical staining of human hippocampus shows strong nuclear and cytoplasmic positivity in neuronal cells.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only. Rabbit IgG Purified Hu E, ICC/IF, P
0.1 mg / €325.00
  OriGene Technologies, Inc.

Casein kinase II subunit alpha antibody

Cell lysates of HeLa (30ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PkCk-2(1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG1 3G1 Purified Hu E, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

Casein kinase II subunit alpha antibody

Cell lysates of HeLa (30ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PkCk-2(1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG1 3G1 Purified Hu E, WB
50 µl / €230.00
  Acris Antibodies GmbH

Casein kinase II subunit alpha (350-391) antibody

Immunoprecipitation of CKII alpha from a RIPA extract of HeLa cells (0.5 mg) with Rabbit anti Human CKII alpha (5 µg/0.5 mg lysate) (AP05321PU-N). The immunoprecipitated CKII alpha was subsequently detected by Western Blotting using a Monoclonal antibody to CKII alpha Rabbit IgG Purified Chk, Hu, Ms, Rt IP, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

Casein kinase II subunit alpha antibody

Western blot analysis of CKII-α antibody (AP20375PU-N) in extracts from HeLa cells. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Casein kinase II subunit alpha pThr360/pSer362 antibody

Western blot of CK2α(Phospho- Thr360/Ser362) antibody (AP26043PU-N) and CK2α antibody in vitro kinase assay. Both purified ERK2 and CK2 were used. CK2α (Phospho-Thr360/Ser362) antibody could recognize ERK2 phosphorylated wild type CK2α and CK2α  when Ser362 was mutated to alanine. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 ml / €320.00
  Acris Antibodies GmbH

Casein kinase II subunit alpha pThr360/pSer362 antibody

Western blot of CK2α(Phospho- Thr360/Ser362) antibody (AP26043PU-N) and CK2α antibody in vitro kinase assay. Both purified ERK2 and CK2 were used. CK2α (Phospho-Thr360/Ser362) antibody could recognize ERK2 phosphorylated wild type CK2α and CK2α  when Ser362 was mutated to alanine. Rabbit Aff - Purified Hu, Ms, Rt WB
50 µl / €240.00
  Acris Antibodies GmbH

Casein kinase II subunit alpha (353-357) antibody

Western blot of CK2α(Phospho-Thr360/Ser362) antibody and CK2αantibody (AP26072PU-N) in vitro kinase assay. Both purified ERK2 and CK2 were used. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 ml / €270.00
  Acris Antibodies GmbH

Casein kinase II subunit alpha (353-357) antibody

Western blot of CK2α(Phospho-Thr360/Ser362) antibody and CK2αantibody (AP26072PU-N) in vitro kinase assay. Both purified ERK2 and CK2 were used. Rabbit Aff - Purified Hu, Ms, Rt WB
50 µl / €230.00
  Acris Antibodies GmbH

Casein kinase II subunit alpha (exon 3) antibody

Detection of human CKII alpha in a RIPA extract of Hela cells treated with either CKII alpha siRNA (a) or vimentin SiRNA (v) by Western blotting with Rabbit anti Human CKII alpha (SP2062P) Rabbit Aff - Purified Hu WB
50 µg / €330.00
  Acris Antibodies GmbH

Casein kinase II subunit alpha antibody

Western Blot analysis of CSNK2A1 expression in transfected 293T cell line (H00001457-T02) by CSNK2A1 MaxPab polyclonal antibody.

Lane 1: CSNK2A1 transfected lysate(45.10 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

Casein kinase II subunit alpha antibody

Western blot analysis of HeLa cell lysate ( 30 µg ) was resolved by SDS - PAGE , transferred to PVDF membrane and probed with CSNK2A1 monoclonal antibody , clone 3G1 ( 1 : 1000 ) ( Cat # MAB1086 ) . Proteins were visualized using a goat anti - mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG1 3G1 Hu E, WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

Casein kinase II subunit alpha antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with CSNK2A1 polyclonal antibody ( Cat # PAB4015 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Hu, Ms E, P, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

Casein kinase II subunit alpha pTyr255 antibody

Western blot analysis of extracts from HeLa cells and Jurkat cells, using Casein Kinase II alpha (Phospho-Tyr255) Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €205.00
  Assay Biotech

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