Caspase-2 antibody

Principal name

Caspase-2 antibody

Alternative names for Caspase-2 antibody

CASP-2, CASP2, ICH1, ICH-1 protease, NEDD2, Neural precursor cell expressed developmentally down-regulated protein 2

SwissProt ID

P29594 (Mouse), P42575 (Human), P55215 (Rat), Q98943 (Chick)

Gene ID

835 (CASP2), 8352517 (G4LUI9), 64314 (Casp2), 12366

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Mky (Monkey), Can (Canine), Chk (Chicken)

Available hosts

Rabbit, Mouse, Rat

Available applications

Enzyme Immunoassay (E), Paraffin Sections (P), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C), Immunoprecipitation (IP), ELISA (detection) (E(detection)), Flow Cytometry (F)

Background of Caspase-2 antibody

Apoptosis occurs during normal cellular development and involves dramatic changes in cellular structure. Disruption of apoptosis may contribute to cancer and other diseases. Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. Caspase-2 has been called ICH-1 in human, CED-3 in C.elegans and NEDD-2 in mouse. Caspase-2 is synthesized as an inactive precursor that is processed in cells undergoing apoptosis. The precursor form of all caspases is composed of a pro-domain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli, including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. Caspase-2 is activated in vitro by caspase-1, caspase-3 and granzyme B. Caspase-2 functions as an upstream apoptosis initiator that participates in the activation of downstream caspases, such as caspase-3, caspase-6 and caspase-7. Caspase-2 is highly expressed in embryonic mouse brain but not in adult neural tissue. Alternative mRNA splicing produces a long (48 kDa) and short (35 kDa) form of caspase-2. These forms show tissue specificity and function either as inducers or suppressors of apoptosis. Mature caspase-2 is 435 amino acids in length cleaved (~50 kDa) and is cleaved upon activation into 10 and 20 kDa subunits.

General readings

Kumar S et al., (1992) Biochem Biophys Res Commun 185:1155-61
Wang L et al., (1994) Cell 78:739-50
Nancy A et al., (1998) Science 281: 1312-1316
Lassus P et al., (2002) Science 297:1352-1354
Baliga BC et al., (2003) J Biol Chem 278: 4899-4905

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Caspase-2 (N-term) antibody

Western blot analysis of caspase-2 in Ramos cells with caspase-2 antibody at (A) 0.5, (B) 1, and (C) 2 ug/mL. Rabbit IgG Aff - Purified Hu, Ms E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Caspase-2 antibody

Western blot analysis of extracts from Jurkat cells, treated with etoposide (25uM, 24hours), using CASP2 (p18, Cleaved-Gly170) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Caspase-2 antibody

Western blot analysis of extracts from rat lung cells and rat heart cells, using CASP2 (p18, Cleaved-Thr325) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Caspase-2 antibody

Antibody to Caspase-2 was used at 1:750 to detect rat caspase-2 in transfected human 292 cell lysates by WB.  Asterisk indicates a 48 kDa caspase-2 protein clearly detected in the rat caspase-2 transfected lysate. Control (lane 1) and transfected (lane 2) lysates were loaded on a 4-20% gel for SDS-PAGE. After primary antibody incubation and washing, a 1:5,000 dilution of HRP conjugated Gt-a-Rabbit IgG  preceded color development using Amersham's substrate system. Rabbit Aff - Purified Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Caspase-2 antibody

Predicted band size: 51kDa. Positive control: Hela cell lysate. Recommended dilution: 1/1000-5000. (Gel: 8%SDS-PAGE Lysate: 40 ug Primary antibody: 1/1000 dilution Secondary antibody: Donkey anti Rabbit IgG - H&L (HRP) at 1/3000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Caspase-2 antibody

Predicted band size: 51kDa. Positive control: Human fetal kidney and lung tissue lysate. Recommended dilution: 1/1000-5000. (Gel: 10%SDS-PAGE Lane1: Human fetal kidney tissue lysate Lane2: Human fetal lung tissue lysate Lysates: 30 ug per lane Primary antibody: 1/950 dilution Secondary antibody: Donkey anti Rabbit IgG - H&L (HRP) at 1/3000 dilution Exposure time: 2 minutes) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Caspase-2 antibody

Western blot analysis of extracts of various cell lines, using CASP2 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

Caspase-2 antibody

Western blot on Ramos (lane 1) and Jurkat cells (lane 2): Cell lysates of Ramos and Jurkat (each 40 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Caspase-2(1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a ECL detection system. Mouse IgG2b 1C10 Purified Hu E, WB
0.1 ml / €410.00
  Acris Antibodies GmbH

Caspase-2 Control Peptide

Caspase-2 Purified
50 µg / €190.00
  Acris Antibodies GmbH

Caspase-2 antibody

Western blot analysis of caspase-2 in untreated (Lane 1,2) and treated (Lane 3,4) cell lysate. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 mg / €370.00
  Acris Antibodies GmbH

Caspase-2 antibody

Immunohistochemistry (IHC) analyzes of Caspase 2 antibody (Cat.-No.: AP01357PU-N) in paraffin-embedded human lung carcinoma tissue Rabbit Aff - Purified Hu E, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Caspase-2 antibody

Figure 1. Formalin-Fixed Paraffin-Embedded (FFPE) on Placenta. Rabbit Aff - Purified Hu, Ms ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

Caspase-2 (C-term) antibody

Caspase 2 Polyclonal Antibody Rabbit IgG Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

Caspase-2 Control Peptide

Caspase-2
50 µg / €160.00
  Acris Antibodies GmbH

Caspase-2 antibody

Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit IgG Purified Hu P, WB
50 µl / €470.00
  Acris Antibodies GmbH

Caspase-2 (C-term) antibody

Western blot analysis of extracts from Hela cells, using anti-Caspase-2L Antibody Cat.-No AP33133PU-N Rabbit IgG Aff - Purified Hu, Ms, Rt IP, WB
0.125 ml / €200.00
  Acris Antibodies GmbH

Caspase-2 antibody

CASP2 polyclonal antibody (A01), Lot # 051116JC01. Western Blot analysis of CASP2 expression in Daoy. Isoform 34.923 kDa Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

Caspase-2 antibody

Western Blot analysis of CASP2 expression in transfected 293T cell line (H00000835-T02) by CASP2 MaxPab polyclonal antibody.

Lane 1: CASP2 transfected lysate(50.70 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

Caspase-2 antibody

Caspase-2 Mouse IgG2a 1C11 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Caspase-2 antibody

Western blot analysis of cell lysates ( each 40 µg ) . Lane 1 : Ramos. Lane 2 : Jurkat. To probed with CASP2 monoclonal antibody , clone 1C10 ( 1 : 500 ) ( Cat # MAB1084 ) . Proteins were visualized using a goat anti - mouse secondary antibody conjugated to HRP and a ECL detection system. Mouse IgG2b 1C10 Hu E, WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

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