Caspase-3 antibody

Principal name

Caspase-3 antibody

Alternative names for Caspase-3 antibody

CASP-3, CASP3, CPP32, CPP-32, Yama protein, Apopain, SCA-1, SCA1, Cysteine protease CPP32, SREBP cleavage activity 1

SwissProt ID

P42574 (Human), P55213 (Rat), P70677 (Mouse), Q08DY9 (Bovin), Q95ND5 (Pig)

Gene ID

836 (CASP3), 12367 (Casp3), 25402

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Por (Porcine), Rb (Rabbit), Hst (Hamster), Mky (Monkey), Sh (Sheep), Chk (Chicken), GP (Guinea Pig), Ye (Yeast), Gt (Goat), Ge (Gerbil)

Available hosts

Mouse, Rabbit, Goat, Rat

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Frozen Sections (C), Immunoprecipitation (IP), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), Flow Cytometry (F)

Background of Caspase-3 antibody

Apoptosis, or programmed cell death, is a common property of all multicellular organisms. The current dogma of apoptosis suggests that the components of the core cell-death machinery are integral to cells and widely conserved across species. Caspases, a family of cysteinyl aspartate-specific proteases, are integral components of the cell death machinery (reviewed in Siegal, 2006; and Lavrik et al, 2005). They play a central role in the initiation and execution of apoptotic cell death and in inflammation. Caspases are typically divided into 3 major groups, depending on the structure of their prodomain and their function. Group 1: inflammatory caspases (caspases 1, 4, 5, 11, 12, 14). Group II: initiator of apoptosis caspases (caspases 2, 8, 9). Group II: effector caspases (caspases 3, 6, 7). Caspases are synthesized as zymogens (ictive pro enzyme precursors which require a biochemical change to become active enzymes) with an N-termil prodomain of variable length followed by a large subunit (p20) and a small subunit (p10). Caspases are activated through proteolytic cleavage at specific asparagine residues that are located within the prodomain, the p10, and p20 subunits. Activation results in the generation of mature active caspases that consist of the heterotetramer p20-p10. Active caspases mediate cell death and inflammation through cleavage of particular cellular substrates that are involved in these processes. The Active/Cleaved Caspase-3 polyclol antisera recognizes the large (~14-21 kDa) and small (~10 kDa) subunits of active/cleaved caspase-3. Whereas the antisera has a strong preference for active/cleaved caspase-3, in some cell or tissue systems or techniques the antisera may also recognize the proform of caspase-3 (~32 kDa).

General readings

1. Costa B  et al. Human glioblastoma multiforme: p53 reactivation by a novel MDM2 inhibitor. PLoS One 8:e72281 (2013). IHC-P .
2. Kou X  et al. Tumor necrosis factor-alpha attenuates starvation-induced apoptosis through upregulation of ferritin heavy chain in hepatocellular carcinoma cells. BMC Cancer 13:438 (2013). WB ; Human .
3. Li J  et al. EZH2-mediated H3K27 trimethylation mediates neurodegeneration in ataxia-telangiectasia. Nat Neurosci 16:1745-53 (2013).

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Caspase-3 (Full length) antibody

AM08377PU-N Caspase-3 antibody staining of Formalin-Fixed Paraffin-Embedded Human Thymus at 5 µg/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Mouse IgG 31A1067  Purified Hu, Ms P, WB
50 µg / €470.00
  Acris Antibodies GmbH

Caspase-3 antibody

Jurkat cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with Biotin anti-Caspase-3 Antibody AM32544BT-N (Clone 4-1-18). Proteins were visualized using an HRP-conjugated Streptavidin and chemiluminescence detection. Mouse IgG2a 4-1-18 Biotin Hu WB
0.1 mg / €320.00
  Acris Antibodies GmbH

Caspase-3 (active) antibody

Western blot analysis of caspase-3 in camptothecin (2 μM) treated Jurkat cells. Rabbit Purified Hu, Ms, Rt P, WB
0.1 mg / €540.00
  Acris Antibodies GmbH

Caspase-3 antibody

Western Blot: Caspase-3 (Pro and Active) Antibody (31A1067) [TA336455] -  Lanes 1, 2 and 3 demonstrate the species crossreactivity of the antibody in human, mouse and rat heart lysate, respectively. Mouse IgG1 31A1067 Purified Hu, Ms, Rt C, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Caspase-3 antibody

Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-CASP3 Rabbit Polyclonal antibody. (TA590291) Rabbit IgG Purified Can, Hu, Mky, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Caspase-3 antibody

Western blot detection of Capsase (19 and 35 kDa) from HEK293 cell extract. Lanes 1 and 2 contain inactive and active Capsase, respectively Mouse IgG2a CPP32 4-1-18 Purified Hu WB
0.2 ml / €325.00
  OriGene Technologies, Inc.

Caspase-3 antibody

(1ug/ml) staining of MOLT4 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Caspase-3 antibody

Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using Caspase 3 (cleaved-Asp175) antibody.The picture on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Caspase-3 antibody

Western blot analysis of extracts from 293 cells, treated with Etoposide (25uM, 60mins), using Caspase 3 (cleaved-Asp175) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Caspase-3 antibody

Western blot analysis of extracts from COLO cells, using CASP3 (p17, Cleaved-Asp175) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Caspase-3 antibody

Western blot analysis of extracts from COS 7 cells, using Caspase 3 (Cleaved-Ser29) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Caspase-3 antibody

Western blot analysis of extracts from NIH/3T3 cells, using OR7E5P antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Caspase-3 (Center) antibody

Immunohistochemistry of Caspase-3 in human tonsil tissue with Caspase-3 antibody at 5 ug/mL. Rabbit IgG Aff - Purified Hu E, P
0.1 mg / €325.00
  OriGene Technologies, Inc.

Caspase-3 antibody

Predicted cell location: Cytoplasm . Positive control: Human thyroid cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CASP3 antibody at dilution 1/15, on the right is treated with the synthetic peptide. (Original magnification:x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Caspase-3 antibody

Predicted band size: 32 kDa . Positive control: K562 cell lysate. Recommended dilution: 1/200-1000. (Gel: 12%SDS-PAGE Lysate: 40 ug Primary antibody: 1/200 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 10 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Caspase-3 antibody

Predicted band size: 32 kDa . Positive control: K562 cell lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lysate: 50 ug Primary antibody: 1/350 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Caspase-3 (C-term) antibody

CASP3 Antibody (C-term) (Cat. #TA324396) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the CASP3 antibody detected the CASP3 protein (arrow). Rabbit Ig Purified Hu F, P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

Caspase-3 antibody

Western blot analysis of CASP3 (Asp175) Antibody (Cat. #TA324797) in CEM cell line lysates (35ug/lane). CASP3 (arrow) was detected using the purified Pab. Rabbit IgG Purified Hu F, P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

Caspase-3 antibody

Western blot analysis of Caspase 3 expression in Etoposide treated NIH-3T3 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Caspase-3 antibody

Western blot analysis of extracts of jurkat cell, using CASP3 antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

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