Caspase-6 antibody

Principal name

Caspase-6 antibody

Alternative names for Caspase-6 antibody

CASP-6, CASP6, MCH2, MCH-2, Apoptotic protease Mch-2

SwissProt ID

O08738 (Mouse), O35397 (Rat), P55212 (Human), Q3T0P5 (Bovin)

Gene ID

839 (CASP6), 12368 (Casp6), 83584

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Rb (Rabbit), Bov (Bovine), Can (Canine), Hst (Hamster), Mky (Monkey), Sh (Sheep), Por (Porcine)

Available hosts

Rabbit, Mouse, Goat

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Western blot / Immunoblot (WB), Immunoprecipitation (IP), ELISA (detection) (E(detection)), Flow Cytometry (F), Frozen Sections (C)

Background of Caspase-6 antibody

Caspase-6 (Cysteine-Requiring Aspartate Proteases) is part of a family of intracellular cysteine proteases that cleave their substrates after aspartic acid residues. These proteases play an integral role in inducing apoptosis in cells. Procaspase-6 (Mch2), a member of the ICE/ced-3 subfamily, is an inactive proenzyme that is activated to form caspase-6 by proteolytic cleavage at certain aspartic acid residues. During cleavage, the N-terminal is removed and the proenzyme is converted into a large (p18) and small (p11) subunits. Caspase-6 has two isoforms produced by alternative splicing. Over-expression of the isoform of caspase-6 without its prodomain can induce apoptosis. The isoform does not seem to display proteolytic activity. Together with caspases-3 and -7, the isoform of caspase-6 is classified as an effector/execution caspase. Caspase-3, caspase-8 and caspase-10 can cleave procaspase-6. Active caspase-6 cleaves several other proteins such as lamins, NuMa and Keratin 18. A possible cleavage of caspases-8 and -10 in cytochrome-C dependent apoptosis was reported recently.

General readings

1. Fernandes-Alnemri, T. et al. Cancer Res. 55: 2737–42,
1995.
2. Srinivasula, SM et al. J Biol. Chem. 271: 27099–106, 1996
3. Slee, EA. et al. J. Cell Biol. 144: 281–292 1999.
4. Takahashi, A. et al. Proc Natl Acad Sci U S A 93: 8395–
400, 1996.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Caspase-6 (Center) antibody

Western blot analysis of Caspase-6 in MCF7 cell lysate with Caspase-6 antibody (IN) at (A) 1 and (B) 2 ug/mL. Rabbit IgG Aff - Purified Hu E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Caspase-6 (N-term) antibody

Western blot analysis of caspase-6 in Jurkat cell lysate with caspase-6 antibody at (A) 0.5, (B) 1, and (C) 2 ug/mL. Rabbit IgG Aff - Purified Hu E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Caspase-6 antibody

Western blot analysis of extracts from 293 cells, treated with Etoposide (25uM, 60mins), using Caspase 6 (cleaved-Asp179) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Caspase-6 antibody

Western blot analysis of extracts from A431 cells, using Caspase 6 (Ab-257) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Caspase-6 antibody

Western blot analysis of extracts from HeLa cells, treated with etoposide (25µM, 1hour), using Caspase 6 (cleaved-Asp162) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Caspase-6 (alpha) antibody

Anti-CASP6 (0.5ug/ml) staining of Human Colon lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Caspase-6 antibody

Anti-CASP6 (0.3ug/ml) staining of Human Colon lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Caspase-6 antibody

Predicted band size: 33 kDa . Positive control: Mouse stomach tissue lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/100 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 40 seconds) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Caspase-6 antibody

Predicted band size: 33 kDa . Positive control: Mouse stomach tissue lysate. Recommended dilution: 1/50-500. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/50 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 30 seconds) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Caspase-6 (C-term) antibody

Western blot analysis of anti-CASP6 Antibody (C-term) (Cat.#TA324510) in mouse liver tissue lysates (35ug/lane). CASP6 (arrow) was detected using the purified Pab. Rabbit Ig Aff - Purified Hu, Ms P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

Caspase-6 antibody

Western blot analysis of CASP6 (arrow) using rabbit polyclonal CASP6 Antibody (N-term) (Cat. #TA324528). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CASP6 gene. Rabbit IgG Purified Hu P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

Caspase-6 antibody

Western blot analysis of extracts of Jurkat cell lines, using CASP6 antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Caspase-6 antibody

Western Blot: Caspase 6 Antibody [TA336324] - analysis of Caspase-6 using Caspase-6 polyclonal antibody. Recombinant catalytically active caspase-6was western blotted with Active/Cleaved Caspase-6 antibody. Theantibody detected both the large and small su Rabbit Purified Can, Hu, Ms, Rt IP, P, WB
50 µl / €325.00
  OriGene Technologies, Inc.

Caspase-6 antibody

Western blot analysis of Caspase 6 (Cleaved-Asp179) expression in Hela cells Rabbit IgG Purified Hu, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Caspase-6 antibody

Figure 1. Western blot analysis using caspase-6 antibody (AM05283PU-N) on MCF-7 cells negative (-) and positive (+) for caspase-3 after treatment for 48 hours with thapsigargin. Mouse IgG1 MCH2 14 1-190 Purified Hu WB
0.1 mg / €360.00
  Acris Antibodies GmbH

Caspase-6 (1-293) antibody

Immunocytochemical detection of Caspase-6 on acetone fixed HEp-II cells with AM26617AF-N Mouse IgG1 3E8 Azide Free Hu, Rt ICC/IF, WB
0.1 mg / €360.00
  Acris Antibodies GmbH

Caspase-6 (active) antibody

Caspase-6 Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 mg / €500.00
  Acris Antibodies GmbH

Caspase-6 Control Peptide

Caspase-6 Purified
50 µg / €190.00
  Acris Antibodies GmbH

Caspase-6 antibody

Western blot analysis of Caspase-6 expression rat kidney tissue lysate. Both full length and the cleaved large fragment have been detected. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 mg / €370.00
  Acris Antibodies GmbH

Caspase-6 antibody

Skeletal muscle: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Aff - Purified Hu ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

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