Caspase-6 antibody
Principal name
Caspase-6 antibody
Alternative names for Caspase-6 antibody
CASP-6, CASP6, MCH2, MCH-2, Apoptotic protease Mch-2
Ncbi ID
NP_001217.2, 9606, NP_954871, NP_001217, NP_033941.3, NM_032992, NM_009811, NM_001226
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Immunoprecipitation (IP), ELISA (detection) (E(detection))
Background of Caspase-6 antibody
Caspase-6 (Cysteine-Requiring Aspartate Proteases) is part of a family of intracellular cysteine proteases that cleave their substrates after aspartic acid residues. These proteases play an integral role in inducing apoptosis in cells. Procaspase-6 (Mch2), a member of the ICE/ced-3 subfamily, is an inactive proenzyme that is activated to form caspase-6 by proteolytic cleavage at certain aspartic acid residues. During cleavage, the N-terminal is removed and the proenzyme is converted into a large (p18) and small (p11) subunits. Caspase-6 has two isoforms produced by alternative splicing. Over-expression of the isoform of caspase-6 without its prodomain can induce apoptosis. The isoform does not seem to display proteolytic activity. Together with caspases-3 and -7, the isoform of caspase-6 is classified as an effector/execution caspase. Caspase-3, caspase-8 and caspase-10 can cleave procaspase-6. Active caspase-6 cleaves several other proteins such as lamins, NuMa and Keratin 18. A possible cleavage of caspases-8 and -10 in cytochrome-C dependent apoptosis was reported recently.
General readings
Nakamura,T., (2004) J. Biol. Chem. 279 (1), 626-634
Western blot analysis of Caspase-6 expression rat kidney tissue lysate. Both full length and the cleaved large fragment have been detected.
Figure 1. Western blot analysis using caspase-6 antibody (AM05283PU-N) on MCF-7 cells negative (-) and positive (+) for caspase-3 after treatment for 48 hours with thapsigargin.
Skeletal muscle: Formalin-Fixed Paraffin-Embedded (FFPE)
Adrenal: Formalin-Fixed Paraffin-Embedded (FFPE)
Figure 1. Formalin-Fixed Paraffin-Embedded (FFPE) on Prostate.
Formalin-fixed paraffin-embedded tissue section of dog cerebellum stained for Active/Cleaved CASP6 expression. Using Active/Cleaved CASP6 polyclonal antibody ( Cat # PAB0244 ) at 1 : 2000. Hematoxylin-eosin counterstain.
Formalin-fixed paraffin-embedded tissue sections of human glioma stained for Active/Cleaved CASP6 expression. Using Active/Cleaved CASP6 polyclonal antibody ( Cat # PAB0244 ) at 1 : 2000. Gemistocytoma ( low grade tumor ) at low ( A ) and high ( B ) magnification. Two examples of anaplastic, high grade ( III ) infiltrating gliomas ( C and D ) . Increased active/cleaved Caspase-6 may be seen in high grade, compared to low grade tumors. Hematoxylin-eosin counterstain.
Western blot analysis of Caspase-6. Recombinant catalytically active CASP6 was western blotted with Active/Cleaved CASP6 polyclonal antibody ( Cat # PAB0244 ) . The antisera detected both the large and small subunits of Active/Cleaved CASP6.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with CASP6 (phospho S257) polyclonal antibody ( Cat # PAB0423 ) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
The CASP6 (phospho S257) polyclonal antibody ( Cat # PAB0423 ) is used in Western blot to detect Phospho-CASP6-S257 in mouse stomach tissue lysate
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with CASP6 polyclonal antibody ( Cat # PAB2445 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
Western blot analysis of CASP6 polyclonal antibody ( Cat # PAB2445 ) in mouse liver tissue lysates ( 35 µg/lane ) . CASP6 ( arrow ) was detected using the purified polyclonal antibody.
Human Jurkat; WB Suggested Anti-SP6 Antibody Titration: 0.2-1 ug/ml. Positive Control: Jurkat cell lysate; SP6 antibody - C-terminal region (ARP50882_P050) in Human Jurkat cells using Western Blot
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
The anti-Phospho-Caspase 6-S257 Pab (Cat.#AP12574PU-N ) is used in Western blot to detect Phospho-Caspase 6-S257 in mouse stomach tissue lysate
Proximity Ligation Analysis (PLA) of protein-protein interactions between CASP6 and APP. HeLa cells were stained with anti-CASP6 rabbit purified polyclonal 1:1200 and anti-APP mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.
CASP6 MaxPab rabbit polyclonal antibody. Western Blot analysis of CASP6 expression in mouse lung.
Western Blot analysis of CASP6 expression in transfected 293T cell line (H00000839-T02) by CASP6 MaxPab polyclonal antibody.Lane 1: CASP6 transfected lysate(33.30 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry of CASP6 in MCF-7 with CASP6 polyclonal antibody ( Cat # PAB13138 ) at 1 µg/ml.
Western blot analysis of CASP6 in MCF-7 cell lysate with CASP6 polyclonal antibody ( Cat # PAB13138 ) at ( A ) 1 and ( B ) 2 µg/ml.
Immunocytochemistry of CASP6 in Jurkat cells with CASP6 polyclonal antibody ( Cat # PAB13139 ) at 2 µg/ml.
Western blot analysis of CASP6 in Jurkat cell lysate with CASP6 polyclonal antibody ( Cat # PAB13139 ) at ( A ) 0.5, ( B ) 1, and ( C ) 2 µg/ml.
Western blot analysis of Casp6 expression in rat kidney tissue lysate.
Small intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human SHSY5Y; . Sample: SH-SY5Y cells 2.0x105 cells . Primary dilution: 1:2500. Secondary Antibody: HRP-conjugated goat anti-rabbit IgG. Secondary dilution: 1:5000. Image Submitted by: Atsuhiro Tanabe. Pharma Sci Kitasato University .; CASP6 antibody - middle region (ARP58994_P050) in Human SHSY5Y cells using Western Blot
Human PANC1; WB Suggested Anti-CASP6 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: PANC1 cell lysate; CASP6 antibody - middle region (ARP58994_P050) in Human PANC1 cells using Western Blot
Immunocytochemical detection of Caspase-6 on acetone fixed HEp-II cells with AM26617AF-N
Western blot analysis of Caspase-6 fragments expression in apoptosis induced Jurkat cells by anti-Fas monoclonel antibody using AM26617AF-N. AM26617AF-N reacts with pro-caspase-6 and activated form.
Immunohistochemical analysis of Caspase 6 p18 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of Caspase 6 p18 staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of Caspase 6 p18 expression in HeLa (A); mouse brain (B); rat kidney (C); NIH3T3 staurosporine-treated (D); Jurkat etoposide-treated (E) whole cell lysates.
Immunohistochemical analysis of Caspase 6 p18 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot analysis of Caspase 6 p18 expression in MCF7 H2O2-treated (A); Jurkat (B); NIH3T3 (C); PC12 (D) whole cell lysates.
Immunohistochemical analysis of Caspase 6 staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of Caspase 6 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of Caspase 6 expression in HepG2 (A); Raw264.7 (B) whole cell lysates.
Western blot analysis of Caspase-6 in MCF7 cell lysate with Caspase-6 antibody (IN) at (A) 1 and (B) 2 ug/mL.
Immunofluorescence of Caspase 6 in MCF7 cells with Caspase 6 antibody at 10 ug/mL.
Immunocytochemistry of caspase-6 in MCF7 with caspase-6 antibody at 1 ug/mL.
Western blot analysis of caspase-6 in Jurkat cell lysate with caspase-6 antibody at (A) 0.5, (B) 1, and (C) 2 ug/mL.
Immunocytochemistry of caspase-6 in Jurkat cells with caspase-6 antibody at 2 ug/mL.
Western blot analysis of extracts from 293 cells, treated with Etoposide (25uM, 60mins), using Caspase 6 (cleaved-Asp179) antibody.The lane on the right is treated with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using Caspase 6 (cleaved-Asp179) antibody.The picture on the right is treated with the synthesized peptide.
Western blot analysis of extracts from A431 cells, using Caspase 6 (Ab-257) antibody.The lane on the right is treated with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Caspase 6 (Ab-257) antibody.The picture on the right is treated with the synthesized peptide.
Immunofluorescence analysis of HuvEc cells, using Caspase 6 (Ab-257) antibody.The picture on the right is treated with the synthesized peptide.
Western blot analysis of extracts from HeLa cells, treated with etoposide (25µM, 1hour), using Caspase 6 (cleaved-Asp162) antibody.The lane on the right is treated with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using Caspase 6 (cleaved-Asp162) antibody.The picture on the right is treated with the synthesized peptide.
Anti-CASP6 (0.5ug/ml) staining of Human Colon lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Anti-CASP6 (0.3ug/ml) staining of Human Colon lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Predicted band size: 33 kDa . Positive control: Mouse stomach tissue lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/100 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 40 seconds)
Predicted band size: 33 kDa . Positive control: Mouse stomach tissue lysate. Recommended dilution: 1/50-500. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/50 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 30 seconds)
Western blot analysis of anti-CASP6 Antibody (C-term) (Cat.#TA324510) in mouse liver tissue lysates (35ug/lane). CASP6 (arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with CASP6 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot analysis of CASP6 (arrow) using rabbit polyclonal CASP6 Antibody (N-term) (Cat. #TA324528). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CASP6 gene.
CASP6 Antibody (N-term) (TA324528)immunohistochemistry analysis in formalin fixed and paraffin embedded human bladder carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CASP6 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Western blot analysis of extracts of Jurkat cell lines, using CASP6 antibody.
Western Blot: Caspase 6 Antibody [TA336324] - analysis of Caspase-6 using Caspase-6 polyclonal antibody. Recombinant catalytically active caspase-6was western blotted with Active/Cleaved Caspase-6 antibody. Theantibody detected both the large and small su
Immunohistochemistry: Caspase 6 Antibody [TA336324] - Formalin-fixed paraffin-embedded tissue section of dog cerebellum stained for Caspase-6 expression at 1:2000. Hematoxylin-eosin counterstain.
Immunohistochemistry-Paraffin: Caspase 6 Antibody [TA336324] - Formalin-fixed paraffin-embedded tissue sections of human gliomas stained for Active/Cleaved Caspase-6 expression using Caspase-6 antibody at 1:2000. Gemistocytoma (low grade tumor) at low (A)
Western blot analysis of Caspase 6 (Cleaved-Asp179) expression in Hela cells
Western blot analysis of extracts from 293 cells treated with Etoposide (25uM, 60min), using Caspase 6 (Phospho-Ser257) antibodyThe lane on the right is treated with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Caspase 6 (Phospho-Ser257) antibody.The picture on the right is treated with the synthesized peptide.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AP00054PU-N | Caspase-6 (active) antibody |
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Rabbit | Aff - Purified | Hu, Ms, Rt | WB |
0.1 mg /
€495.00
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| OriGene Technologies GmbH | |||||||
