Caspase-8 / FLICE antibody

Principal name

Caspase-8 / FLICE antibody

Alternative names for Caspase-8 / FLICE antibody

CASP-8, CASP8, MCH5, CAP4, ICE-like apoptotic protease 5, MORT1-associated CED-3 homolog, FADD-homologous ICE/CED-3-like protease, FADD-like ICE, Apoptotic cysteine protease, Apoptotic protease Mch-5

SwissProt ID

O89110 (Mouse), Q14790 (Human), Q9JHX4

Gene ID

841 (CASP8), 12370 (Casp8)

Available reactivities

Hu (Human), Bov (Bovine), Mky (Monkey), Ms (Mouse), Rt (Rat), Ge (Gerbil), Prim (Primate)

Available hosts

Mouse, Rabbit, Rat

Available applications

Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Flow Cytometry (F), Frozen Sections (C), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of Caspase-8 / FLICE antibody

Apoptosis, or programmed cell death, is a common property of all multicellular organisms. The current dogma of apoptosis suggests that the components of the core cell-death machinery are integral to cells and widely conserved across species. Caspases, a family of cysteinyl aspartate-specific proteases, are integral components of the cell death machinery (reviewed in Siegal, 2006; and Lavrik et al, 2005). They play a central role in the initiation and execution of apoptotic cell death and in inflammation. Caspases are typically divided into 3 major groups, depending on the structure of their prodomain and their function. Group 1: inflammatory caspases (caspases 1, 4, 5, 11, 12, 14). Group II: initiator of apoptosis caspases (caspases 2, 8, 9). Group II: effector caspases (caspases 3, 6, 7). Caspases are constitutively expressed in almost all cell types as inactive proenzymes (zymogens: enzyme precursors which require a biochemical change to become active enzymes) that are processed and activated in response to a variety of pro-apoptotic or inflammatory stimuli. The procaspases (32-56 kDa) contain four domains: an N-terminal prodomain (2-25 kDa), a large subunit (p20: 17-21 kDa), a small subunit (p10: 10-13 kDa) and a short linker region between the large and small subunits. Caspase activation involves proteolytic processing of the proenzyme at specific aspartate residues between the domains. This results in removal of the prodomain as well as the linker region and formation of a heterodimer containing one large and one small subunit (p20-p10). The active caspase is a tetramer composed of two heterodimers (p202-p102). Active caspases mediate cell death and inflammation through cleavage of particular cellular substrates that are involved in these processes. Multiple isoforms of caspase 8 have been identified, including caspase 8a [496 amino acids (aa), GenBank no. NP_001219.2], caspase 8b (479 aa, Gen Bank no. NP_203519.1), caspase 8c (464 aa, GenBank no. NP_203520.1), and caspase 8L (538 aa, GenBank no. gbAAL87628.1). Truncated caspase-8 forms have also been described. Users are encoraged to go to the NCBI ACE View Data Base for more information. http://www.ncbi.nih.gov/IEB/Research/Acembly/ Caspase-8 may be observed on Western blots at varying apparent molecular weights depending on isoform, truncation, or cleavage forms present.

General readings

General / background references:Martin DA et al. Membrane oligomerization and cleavage activates the caspase-8 (FLICE/MACHalpha1) death signal. J Biol Chem 273:4345-9 (1998). PubMed PMID: 9468483
Hu S et al. I-FLICE, a novel inhibitor of tumor necrosis factor receptor-1- and CD-95-induced apoptosis. J Biol Chem 272:17255-7 (1997). PubMed PMID: 9211860
Muzio M et al. FLICE, a novel FADD-homologous ICE/CED-3-like protease, is recruited to the CD95 (Fas/APO-1) death--inducing signaling complex. Cell 85:817-27 (1996). PubMed PMID: 8681377

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Caspase-8 / FLICE (N-term) antibody

Human stomach stained with Anti-Caspase-8 antibody (Cat# AP15396PU). Rabbit Purified Hu P
0.5 ml / €410.00
  Acris Antibodies GmbH

Caspase-8 / FLICE (N-term) antibody

Human stomach stained with Anti-Caspase-8 antibody (Cat# AP15396PU). Rabbit Purified Hu P
1 ml / €580.00
  Acris Antibodies GmbH

Caspase-8 / FLICE (N-term) antibody

Human stomach stained with Anti-Caspase-8 antibody (Cat# AP15396PU). Rabbit Purified Hu P
0.1 ml / €190.00
  Acris Antibodies GmbH

Caspase-8 / FLICE (C-term) antibody

Western blot analysis of CASP8 antibody (C-term) (Cat. #TA324849) in Hela cell line lysates (35ug/lane). CASP8 (arrow) was detected using the purified Pab. Rabbit Hu WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

Caspase-8 / FLICE antibody

Immunohistochemical analysis of paraffin-embedded liver cancer tissues using CASP8 mouse mAb with DAB staining. Mouse IgG1 1H11 Ascites Hu, Mky, Ms, Rt E, F, P, WB
0.1 ml / €430.00
  Acris Antibodies GmbH

Caspase-8 / FLICE (C-term) antibody

Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Purified Hu ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

Caspase-8 / FLICE (C-term) antibody

Western blot analysis of caspase-8 in Jurkat cell lysate with caspase-8 antibody at (A) 0.5, (B) 1, and (C) 2 ug/mL. Rabbit IgG Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Caspase-8 / FLICE antibody

Western blot analysis of Caspase-8 in HT-29 cell lysate with Caspase-8 antibody (IN) at 1 µg/ml in (A) the presence or (B) the absence of blocking peptide. Rabbit IgG Aff - Purified Hu, Ms E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Caspase-8 / FLICE antibody

Sample(30 ug whole cell lysate). A:HeLa S3. 7.5% SDS PAGE. TA308860 diluted at 1:500 Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Caspase-8 / FLICE antibody

Western blot analysis of extracts from 293 cells, treated with etoposide (25uM, 1hour), using CASP8 (Cleaved-Asp384) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Caspase-8 / FLICE antibody

Predicted band size: 55 kDa . Positive control: 293, Hela and HT29 cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 10%SDS-PAGE Lane1,2,3: 293, Hela and HT29 cells lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Caspase-8 / FLICE antibody

Western blot analysis of Caspase 8 expression in TNF-a treated NIH-3T3 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Caspase-8 / FLICE antibody

Western blot analysis of HeLa cells treated with cycloheximide (CHX) and TNF-alpha using CASP8 antibody. Rabbit IgG Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Caspase-8 / FLICE antibody

Immunohistochemistry: Caspase 8 Antibody [TA336325] - Formalin-fixed, paraffin-embedded sections from a brain tissue array stained for Caspase-8 expression using TA336325 at 1:2000. A. Normal brain stem (1) and cortex (2). B. Higher magnification of corte Rabbit Purified Ge, Hu, Ms, Rt C, ICC/IF, IP, P, WB
50 µl / €325.00
  OriGene Technologies, Inc.

Caspase-8 / FLICE antibody

Western Blot: Caspase 8 Antibody (90A992) [TA336388] - Western blot analysis of Caspase-8 in Jurkat cells using Caspase-8 antibody at 1 ug/ml. Cells were treated with 2 uM staurosporine for different time periods. Caspase-8 activation is detected in weste Mouse IgG1 90A992 Purified Hu, Prim F, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Caspase-8 / FLICE antibody

Western Blot: Caspase 8 Antibody (FLICE 4-1-20) [TA336653] - Detection of Caspase 8 (59 kDa) from HEK293 cell extract using TA336653 (1:500). Lanes 1 and 2 contain inactive and active Caspase 8, respectively. Mouse IgG1 FLICE 4-1-20 Purified Hu WB
0.2 ml / €325.00
  OriGene Technologies, Inc.

Caspase-8 / FLICE antibody

ELISA: Caspase 8 Antibody Rabbit IgG Purified Hu E
0.1 mg / €325.00
  OriGene Technologies, Inc.

Caspase-8 / FLICE (10 kDa subunit) antibody

Caspase-8 / FLICE Mouse IgG1 C502S Aff - Purified Hu IP, WB
0.1 mg / €500.00
  Acris Antibodies GmbH

Caspase-8 / FLICE antibody

Figure 1. Western blot analysis using caspase-8 antibody (AM05285PU-N) on MCF-7 cells negative (-) and positive (+) for caspase-3 after treatment for 48 hours with thapsigargin. Antibody detects procaspase and one cleavage product Mouse IgG FLICE 1 1-37 Purified Hu WB
0.1 mg / €360.00
  Acris Antibodies GmbH

Caspase-8 / FLICE antibody

Figure 1. Western blot analysis using caspase-8 antibody (AM05286PU-N) on MCF-7 cells negative (-) and positive (+) for caspase-3 after treatment for 48 hours with thapsigargin. Antibody detects procaspase and one cleavage product. Mouse IgG FLICE 3 Purified Hu WB
0.1 mg / €360.00
  Acris Antibodies GmbH

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