Caspase-9 antibody

Principal name

Caspase-9 antibody

Alternative names for Caspase-9 antibody

CASP-9, CASP9, MCH6, ICE-LAP6, APAF-3, APAF3, ICE-like apoptotic protease 6, Apoptotic protease Mch-6, Apoptotic protease-activating factor 3

SwissProt ID

P55211 (Human), Q8C3Q9 (Mouse)

Gene ID

842 (CASP9), 12371 (Casp9)

Available reactivities

Bov (Bovine), Can (Canine), Hst (Hamster), Hu (Human), Mky (Monkey), Ms (Mouse), Por (Porcine), Rb (Rabbit), Rt (Rat), Ge (Gerbil)

Available hosts

Rabbit, Goat, Mouse

Available applications

Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Paraffin Sections (P), Frozen Sections (C), Enzyme Immunoassay (E)

Background of Caspase-9 antibody

Caspase 9 (also known as ICE-like apoptotic protease 6 (ICE-LAP6), apoptotic protease Mch-6, and apoptotic protease activating factor 3 (Apaf-3)) is a member of the peptidase family C14 that contains a CARD domain. This caspase is active as a heterotetramer and has been reported to have two isoforms. Pro-Caspase 9 has been reported to be approximately 47 kD. This caspase is present in the cytosol and, upon activation, translocates to the mitochondria. Caspase 9 is involved in the caspase activation cascade responsible for apoptosis execution and cleaves/activates Caspase 3 and Caspase 6. Caspase 9 is inhibited by the dominant negative isoform, Bcl-XL, c-IAP1, c-IAP2, XIAP, and Livin. This caspase becomes activated when recruited to Apaf-1/cytochrome c complex, and following cleavage by Apaf-1, granzyme B, Caspase 3, possibly Caspase 8 and Caspase 10 into large p37 and small p10 subunits. Caspase 9 interacts with BIRC7 and has been shown to cleave PARP and vimentin.  
Structure: Peptidase family C14, CARD domain. Heterotetramer, two isoforms, pro-Casp9 47 kD, active cleaved from Casp9 37 kD. 
Distribution:
 Cytoplasm, when activated mitochondrial.
Function: Involved in caspase activation cascade responsible for apoptosis execution; cleaves/activates Casp3, Casp6.  
Regulation: Inhibited by dominant negative isoform, Bcl-XL, c-IAP1, c-IAP2, XIAP, Livin. Activated when recruited to Apaf-1/cytochrome c complex, cleaved by Apaf-1, granzyme B, Casp3, possibly Casp8 and -10 into large p37 and small p10 subunits.
Interaction:
BIRC7; cleaves PARP, vimentin.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Caspase-9 (N-term) antibody

A431 cell extract was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal Caspase-9 Antibody Cat.-No AM05287PU-S (Clone 96-2-22). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence system. Mouse IgG1 96-2-22 Purified Hu IP, WB
0.1 mg / €310.00
  Acris Antibodies GmbH

Caspase-9 (N-term) antibody

A431 cell extract was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal Caspase-9 Antibody Cat.-No AM05287PU-S (Clone 96-2-22). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence system. Mouse IgG1 96-2-22 Purified Hu IP, WB
25 µg / €210.00
  Acris Antibodies GmbH

Caspase-9 antibody

Western blot analysis of Caspase 9 with Rat kidney tissue lysate. Rabbit Purified Bov, Can, Hst, Hu, Mky, Ms, Por, Rb, Rt WB
0.1 mg / €390.00
  Acris Antibodies GmbH

Caspase-9 (299-318) antibody

Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Purified Hu E, ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

Caspase-9 antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between XIAP and CASP9 HeLa cells were stained with anti-XIAP rabbit purified polyclonal 1:1200 and anti-CASP9 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Mouse IgG1 3B8-4G2 Purified Hu E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Caspase-9 antibody

Western blot analysis of caspase-9 in HeLa whole cell lysate with caspase-9 antibody at 1:1000 dilution. Rabbit IgG Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Caspase-9 antibody

Western blot analysis of caspase-9 in HeLa whole cell lysate with Caspase-9 antibody at 1:1000 dilution Rabbit IgG Aff - Purified Hu E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Caspase-9 antibody

Sample (30 ug of whole cell lysate). A: 293T. 10% SDS PAGE. TA308861 diluted at 1:1000. Rabbit IgG Aff - Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Caspase-9 antibody

The pro and active forms of Caspase 9 detected in active 293 cell lysate (lane 2). Lane 1: inactive 293 cell lysate. Mouse IgG1 LAP6 96-2-22 Purified Hu WB
0.2 ml / €325.00
  OriGene Technologies, Inc.

Caspase-9 antibody

Western blot analysis of extracts from 293 cells, treated with Etoposide (25uM, 60mins), using Caspase 9 (Cleaved-Asp315) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Caspase-9 antibody

Predicted cell location: Cytoplasm. Positive control: Human cervical and stomach cancer tissue. Recommended dilution: 1/50-150 The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue  using CASP9 antibody at dilution 1/50, on the right is immunohistochemistry of paraffin-embedded Human stomach cancer tissue using CASP9 antibody at dilution 1/50. (Original magnification: x100) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Caspase-9 antibody

Western blot analysis of Hela cell and mouse liver tissue using TA321308(CASP9 Antibody) at dilution 1/750 Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Caspase-9 antibody

Predicted cell location: Nucleus,Cytoplasm. Positive control: Human colon and lung cancer tissue. Recommended dilution: 1/100-300  The image on the left is immunohistochemistry of paraffin-embedded human colon cancer tissue using CASP9 (active) antibody at dilution 1/80, on the right is treated with the synthetic peptide. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Caspase-9 (Center) antibody

CASP9 Antibody (Center) (Cat. #TA325012) western blot analysis in Hela cell line and mouse L929 tissue lysates (35ug/lane).This demonstrates the CASP9 antibody detected the CASP9 protein (arrow). Rabbit Ig Purified Hu WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

Caspase-9 antibody

Western blot analysis of Caspase 9 expression in TNF-a treated NIH-3T3 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Caspase-9 antibody

Western blot analysis of extracts of various cell lines, using CASP9 antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Caspase-9 antibody

Immunohistochemistry-Paraffin: Caspase 9 Antibody [TA336326] - Analysis Human Brain sections stained for cleaved Caspase-9 expression using this antibody at 1:2000. A. Section from a patient 24 hr after head trauma. B. Control: section from a patient with Rabbit Purified Can, Ge, Hu, Ms, Rt IP, P
50 µl / €325.00
  OriGene Technologies, Inc.

Caspase-9 antibody

Simple Western: Caspase 9 Antibody [TA336327] - Simple Western lane view shows a specific band detected for Caspase 9 in HeLa lysate.  This experiment was performed under reducing conditions using the Wes or Sally Sue separation system 12-230kDa (or 66-44 Rabbit Purified Can, Ge, Hu, Ms, Rt IP, P, WB
50 µl / €325.00
  OriGene Technologies, Inc.

Caspase-9 antibody

Western blot detection of Caspase-9 in A549,MCF7,Jurkat,K562 and Hela cell lysates using Caspase-9 mouse mAb (1:1000 diluted).Predicted band size:49/37KDa.Observed band size:49KDa. Mouse IgG1 1D1-F2-E9 Purified WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Caspase-9 antibody

Western blot analysis of Caspase 9 (Cleaved-Asp353) expression in NIH/3T3 cells treated with etoposide Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

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