CaSR antibody

Principal name

CaSR antibody

Alternative names for CaSR antibody

Extracellular calcium-sensing receptor, GPRC2A, PCAR1, Parathyroid Cell calcium-sensing receptor

SwissProt ID

O62714 (Pig), P35384 (Bovin), P41180 (Human), P48442 (Rat), Q9QY96 (Mouse)

Gene ID

846 (CASR), 24247 (Casr)

Available reactivities

Bov (Bovine), Hu (Human), Rt (Rat), Ms (Mouse), Rb (Rabbit), Mky (Monkey), Por (Porcine), Bt (Bat), Eq (Equine), Can (Canine), Bear

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), Frozen Sections (C), Flow Cytometry (F), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Radioimmunoassay (R)

Background of CaSR antibody

The extracellular calcium sensing and homeostasis mechanism consist of a G-protein coupled receptor linked to gq and Gi and phosphoinositide turnover and inhibition of parathyroid hormone (PTH) secretion. In cells, the calcium homeostasis is via voltage-dependent L-type calcium channel and calcium sensing receptor (CaSR). The CaSR gene is derived from the CaSR gene expressed in parathyroid glands, CaSR is expressed in at least 2 variant isoforms CaSR and CaSRb. The CaSr senses small changes in the circulating Ca concentrations and couples this information to intracellular signaling pathways that modify . of CaThe activation of CaSR led to inhibition of PTH secretion and stimulation of calcitonin secretion in human medularyC cells (1). Defects in the human Ca(2+)-sensing receptor gene have been shown to cause familial hypocalciuric hypercalcaemia and neonatal severe hyperparathyroidism. Several mutations in CaSR gene caused a missense mutation (228Arg-->Gln, 39Thr-->Met, 144Gly-->Glu, 63Arg-->Met, and 67Arg-->Cys) that encodes a non-conservative amino acid alteration. These mutations are each predicted to be in the Ca(2+)-sensing receptor’s large extracellular domain (2). CaSR are expressed in kidney cells, where the receptor negatively regulates calcium reabsorption. Mutation is this gene further augments the hyperclciurea increase and risk of renal complications. A similar Ca sensing receptor has been cloned in osteoblasts (Ob-CaSR) which differs in coupling to serum response factors, the Ob-CaSR activates ternary complex factor (3).
CaSR is expressed in kidney, but not in brain, lung, liver, heart, skeletal muscle, or placenta. The CaSR is member of 7TMD family of GPCRs, that undergoes N-glycosylation and ubiquitination by RNF19A; which induces proteasomal degradation. The CaSR is a 120kDa protein (1078aa) expressed in the PTH-producing chief cells of the parathyroid gland and in kidneys. The ability of CaSR to sense cations and amino acids and function like a G-protein coupled receptor suggests that it may belong to the family of receptors characterized by an evolutionarily conserved amino acid sensing motif (ANF) linked to an intra-membranous 7 transmembrane loop region.

General readings

1. Barradas AM  et al. A calcium-induced signaling cascade leading to osteogenic differentiation of human bone marrow-derived mesenchymal stromal cells. Biomaterials 33:3205-15 (2012). ICC/IF ; Human .

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CaSR (N-term, extracel. dom.) antibody

Anti-CASR antibody IHC of human parathyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Aff - Purified Bear, Can, Eq, Hu, Mky, Por P
50 µg / €379.00
  OriGene Technologies, Inc.

CaSR antibody

Western Blot: Calcium Sensing Receptor Antibody (5C10, ADD) [NB120-19347] - In HEK7-2 cell extract using NB120-19347. Figure 2 shows Immunofluorescence of Bovine CE (Corneal Epithelium) Limbus tissue extracts using NB120-19347. Mouse IgG2a 5C10, ADD Aff - Purified Bov, Hu, Rt C, E, F, ICC/IF, P, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

CaSR (N-term) antibody

Human placenta stained with anti-CaSR, Cat# AP15386PU Rabbit Purified Hu, Rt P, WB
0.5 ml / €360.00
  Acris Antibodies GmbH

CaSR (N-term) antibody

Human placenta stained with anti-CaSR, Cat# AP15386PU Rabbit Purified Hu, Rt P, WB
1 ml / €520.00
  Acris Antibodies GmbH

CaSR (N-term) antibody

Human placenta stained with anti-CaSR, Cat# AP15386PU Rabbit Purified Hu, Rt P, WB
0.1 ml / €190.00
  Acris Antibodies GmbH

CaSR antibody

Detection of Calcium Sensing Receptor in transfected 293 cell lysate. ECL exposure, 5 minutes. In this photo, the protein has aggregated and thus the higher MW band as expected. Mouse IgG1 HL 1499 Purified Hu, Ms, Rb, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CaSR antibody

Western blot analysis of Calcium Sensing Receptor expression in LOVO whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CaSR antibody

Western blot analysis of Colo205 (lanes 1 and 3), rat liver (lanes 2 and 4) and mouse kidney (lanes 5 and 6) lysates: 1,2,5. Anti-Calcium Sensing Receptor (extracellular) antibody (1:200). 3,4,6. Anti-Calcium Sensing Receptor (extracellular) antibody, preincubated with the control peptide antigen. Rabbit Purified Hu, Ms, Rt WB
0.2 ml / €435.00
  OriGene Technologies, Inc.

CaSR antibody

Anti-CASR antibody IHC of human parathyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Purified Hu
50 µg / €379.00
  OriGene Technologies, Inc.

CaSR antibody

Anti-CASR antibody IHC of human parathyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Purified Bt, Eq, Hu, Mky, Rb, Rt
50 µg / €379.00
  OriGene Technologies, Inc.

CaSR antibody

Western blot analysis of CASR in EL4 cell lysate with CASR antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide. Rabbit IgG Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CaSR Control Peptide

CaSR
0.25 mg / €250.00
  Acris Antibodies GmbH

CaSR (47-69) antibody

CaSR Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

CaSR antibody

Immunohistochemistry analyzes of CaR antibody (AP20293PU-N) in paraffin-embedded human brain tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

CaSR (N-term) antibody

Human Placenta (formalin-fixed, paraffin-embedded) stained with CASR antibody AP22631PU-N at 15 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rabbit Purified Hu, Rt P, WB
50 µg / €470.00
  Acris Antibodies GmbH

CaSR antibody

Western Blot analysis of CASR expression in transfected 293T cell line (H00000846-T01) by CASR MaxPab polyclonal antibody.

Lane 1: CASR transfected lysate(120.60 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

CaSR antibody

Detection limit for recombinant GST tagged CASR is approximately 0.3ng/ml as a capture antibody. Mouse IgG2a 3F12 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

CaSR antibody

Detection of CASR in transfected 293 cell lysate using CASR monoclonal antibody, clone HL 1499 ( Cat #MAB2357 ).
In this photo, the protein has aggregated and thus the higher MW band as expected. Mouse IgG1 HL 1499 Hu, Ms, Rb, Rt WB
0.1 ml / €470.00
  Abnova Taiwan Corp.

CaSR antibody

Immunohistochemical staining of Casr on formalin fixed, paraffin embedded human placenta with Casr polyclonal antibody. Rabbit Hu P, WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

CaSR pThr888 antibody

Immunofluorescence analysis of HeLa cells, using Calcium Sensing Receptor (Phospho-Thr888) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
50 µg / €205.00
  Assay Biotech

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