Catenin alpha-1 / CTNNA1 antibody

Principal name

Catenin alpha-1 / CTNNA1 antibody

Alternative names for Catenin alpha-1 / CTNNA1 antibody

Alpha E-catenin, Alpha Catenin, Cadherin-associated protein, NY-REN-13 antigen

SwissProt ID

P26231 (Mouse), P35221 (Human), Q3MHM6 (Bovin), Q59I72 (Rabit), Q9PVF8

Gene ID

1495 (CTNNA1), 12385 (Ctnna1)

Available reactivities

Can (Canine), Hu (Human), Ms (Mouse), Rt (Rat), Chk (Chicken), Por (Porcine)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Flow Cytometry (F)

Background of Catenin alpha-1 / CTNNA1 antibody

The distinct peripheral cytosolic proteins, alpha, beta and gamma-catenin (102, 94 and 86 kDa) found in many tissues bind to the conserved cytoplasmic tail domain of the cell-adhesion cadherins. Catenins link E-cadherin to other integral membrane or cytoplasmic proteins and are modulated by Wnt-1 proto-oncogene. They are good candidates for mediating transduction of cell-cell contact positional signals to the cell interior. Absence of alpha-catenin is found in certain tumor cell lines and reduced levels in certain human carcinomas. Beta-catenin binds directly to the cytoplasmic tail of E-cadherin. It binds to the N-terminus of alpha-catenin and interacts with the protein product of the tumor suppressor gene APC. This interaction involves a 15-aa repeat in the APC. Beta-catenin cell levels seem to be controlled by APC. The central core region of beta-catenin is involved in mediation of cadherin-catenin complex interaction with EGFR.

General readings

Yutaka Shimoyama, Cancer Res., Oct 1992; 52: 5770 - 5774.
Griet Vanpoucke, Nucleic Acids Res., Aug 2004; 32: 4155 - 4165.
Jolanda van Hengel, J. Cell Biol., Jun 1997; 137: 1103.
Masahiko Itoh, J. Cell Biol., Jul 1997; 138: 181.

Items 1 to 20 of 36 total

per page
Page:
  1. 1
  2. 2

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Catenin alpha 1/2 antibody

Immunohistochemical analysis of Catenin alpha 1/2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Chk, Hu, Ms, Por, Rt P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

Catenin alpha 1/2 antibody

Immunohistochemical analysis of Catenin alpha 1/2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Chk, Hu, Ms, Por, Rt P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

Catenin alpha 1/2 antibody

Immunohistochemical analysis of Catenin alpha 1/2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Chk, Hu, Ms, Por, Rt P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

Catenin alpha-1 / CTNNA1 antibody

TA302443 (1µg/ml) staining of human colon lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Catenin alpha-1 / CTNNA1 antibody

Predicted band size: 100 kDa . Positive control: A431 cell lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/600 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 30 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Catenin alpha-1 / CTNNA1 antibody

Predicted cell location: Cytoplasm. Positive control: Human brain and thyroid cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded human brain tissue using CTNNA1 antibody at dilution 1/15, on the right is treated with the fusion protein. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Catenin alpha-1 / CTNNA1 antibody

Predicted band size: 100 kDa . Positive control: Hela cells untreated or treated with starvation lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lane1: Hela cells treated with starvation lysate Lane2: Hela cells untreated with starvation lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Catenin alpha-1 / CTNNA1 (N-term) antibody

Western blot analysis of CTNA1 antibody (N-term) (Cat. #TA324851) in mouse bladder tissue lysates (35ug/lane). CTNA1 (arrow) was detected using the purified Pab. Rabbit Hu, Ms WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

Catenin alpha-1 / CTNNA1 antibody

Western blot analysis of extracts of various cell lines, using CTNNA1 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

Catenin alpha-1 / CTNNA1 antibody

Western blot analysis of Catenin a1 expression in HeLa cell lysate Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Catenin alpha-1 / CTNNA1 (incl. pos. control) antibody

Detection of endogenous alpha catenin: Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab a-Cat-15D9 (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1: A431; lane 2: A549; lane 3: SKOV3; lane 4: OVCAR5; lane 5: HaCaT; lane 6: PC3; lane 7: HeLa; lane 8: HepG2 Mouse IgG1 15D9 Purified Can, Hu, Ms, Rt E, ICC/IF, IP, WB
0.1 mg / €380.00
  Acris Antibodies GmbH

Catenin alpha-1 / CTNNA1 antibody

Catenin alpha-1 / CTNNA1 Mouse IgG1 1G5 Purified Hu F, ICC/IF, IP, WB
0.1 mg / €400.00
  Acris Antibodies GmbH

Catenin alpha-1 / CTNNA1 antibody

Figure 1. Western blot analysis of extract from A431 cells, untreated or treated with EGF (200ng/ml, 30min), using, Alpha-Catenin Antibody (#AP0876PU) and Alpha-Catenin (Phospho-Ser641) Antibody (#AP09467PU). Rabbit Aff - Purified Hu, Ms WB
0.1 ml / €270.00
  Acris Antibodies GmbH

Catenin alpha-1 / CTNNA1 antibody

Figure 1. Western blot analysis of extract from A431 cells, untreated or treated with EGF (200ng/ml, 30min), using, Alpha-Catenin Antibody (#AP0876PU) and Alpha-Catenin (Phospho-Ser641) Antibody (#AP09467PU). Rabbit Aff - Purified Hu, Ms WB
50 µl / €230.00
  Acris Antibodies GmbH

Catenin alpha-1 / CTNNA1 pSer641 antibody

Western blot analysis of extracts from Hela cells untreated or treated with starvation using Alpha-Catenin pSer641 antibody (Cat.-No AP09467PU). Rabbit Aff - Purified Hu, Ms WB
0.1 ml / €320.00
  Acris Antibodies GmbH

Catenin alpha-1 / CTNNA1 pSer641 antibody

Western blot analysis of extracts from Hela cells untreated or treated with starvation using Alpha-Catenin pSer641 antibody (Cat.-No AP09467PU). Rabbit Aff - Purified Hu, Ms WB
50 µl / €240.00
  Acris Antibodies GmbH

Catenin alpha-1 / CTNNA1 (C-term) antibody

Human Heart: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu, Ms, Rt E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

Catenin alpha-1 / CTNNA1 antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between ARRB2 and CTNNA1. HeLa cells were stained with anti-ARRB2 rabbit purified polyclonal 1:1200 and anti-CTNNA1 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Mouse IgG1 4G6 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Catenin alpha-1 / CTNNA1 antibody

CTNNA1 polyclonal antibody ( Cat # PAB7024 ) ( 1 µg/ml ) staining of human colon lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

Catenin alpha-1 / CTNNA1 antibody

Western blot analysis of extract from A-431 cells, untreated or treated with EGF ( 200ng/ml, 30min ), using CTNNA1 polyclonal antibody ( Cat # PAB12225, Lane 1 and 2 ). Rabbit Hu, Ms WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

Items 1 to 20 of 36 total

per page
Page:
  1. 1
  2. 2
  • LinkedIn