Catenin beta-1 antibody

Principal name

Catenin beta-1 antibody

Alternative names for Catenin beta-1 antibody

CTNNB1, CTNNB, Beta-catenin

SwissProt ID

P26233 (Xenla), P35222 (Human), Q02248 (Mouse), Q0VCX4 (Bovin), Q7ZU14, Q9WU82 (Rat)

Gene ID

1499 (CTNNB1), 30265 (ctnnb1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Bov (Bovine), Chk (Chicken), Por (Porcine), Rb (Rabbit), Ze (Zebrafish), African clawed frog, Mam (Mammalia), Fi (Fish), Xen (Xenopus), Hst (Hamster), Mky (Monkey), Sh (Sheep), Prim (Primate)

Available hosts

Rabbit, Mouse, Goat

Available applications

Western blot / Immunoblot (WB), Frozen Sections (C), Immunoprecipitation (IP), Paraffin Sections (P), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Dot blot (Dot), Flow Cytometry (F), Luminex (LMNX), ChIP assay (ChIP)

Background of Catenin beta-1 antibody

Cell adhesion is important during development, as well as in sorting of cells, induction of cellular morphogenesis and maintenance of tissue integrity.1-3 Ca2+-dependent adhesion of cells involves a multifunctional family of transmembrane glycoproteins termed cadherins that regulate homophilic interactions in cells. These interactions initiate a cascade of events that leads to the structural and functional reorganizations of cells. Cadherins function is mediated by both specific binding of extracellular domains at the cell surface and interaction with components of the cytoplasm. These components include alpha-, beta- and gamma-catenin (plakoglobin) that bind to the cytoplasmic domain of cadherins.4 Beta-catenin (92-97 kDa) shares 70% sequence identity to a protein encoded by Drosophila armadillo, a segment polarity gene.5-8 Beta-catenin binds to a diverse set of proteins including the presenilins, epidermal growth factor receptor (EGF-R), the actin-binding protein fascin and the transcription factor Teashirt.9-10 Beta-catenin is composed of a series of proteinprotein interaction motifs that allow it to function as a scaffold. The N-terminus domain, containing the binding site for alpha-catenin and the phosphorylation sites, is recognized by GSK3Beta. The C-terminus contains the transcriptional activation domain and the binding site for Teashirt.9-10 Beta-catenin translocates into the nucleus, where it complexes with transcription factors of the LEF-1 family and thus regulates the expression of specific genes. By playing such a dual function, i.e. a structural role in cell junctions and a regulatory role in the nucleus, beta-catenin can transduce changes in cell adhesion and junction formation to control transmembrane signaling and gene expression.1, 11 Beta-catenin-mediated signaling depends on its accumulation and subsequent translocation into the nucleus. The level of beta-catenin is regulated by its association with the tumor suppressor molecule Adenomatous Polyposis Coli (APC), axin, and GSK3Beta. In this complex, GSK3Beta phosphorylates beta-catenin at multiple serine or threonine residues present in the amino terminal region of beta-catenin,1 thereby marking beta-catenin for degradation by the proteasome pathway. The significance of beta-catenin phosphorylation for its stability is most clearly manifested in several types of human cancers. In cells expressing mutant APC, common in human colon cancer and melanoma, beta-catenin accumulates due to the failure of its degradation.1 Moreover, a single amino acid mutation at one of the four critical serine or threonine residues (Ser33, -37 and -45 and Thr-41) at the amino terminal region in the consensus GSK3Beta phosphorylation site, results in deregulated accumulation of beta-catenin and thereby increased signaling through the TCF/beta-catenin transcriptional complex, which contributes to tumorigenesis. Immunofluorescence studies indicate that phosphorylated beta-catenin accumulates in the nuclei of Madin-Darby Canine Kidney (MDCK) and BCAP (human breast cancer) cells and is transiently associated with adherens junctions.12 It was recently shown that phosphorylation of Ser45 and Thr41 precedes the phosphorylation of Ser33 and Ser37. Phosphorylation is mediated by casein kinase IAlph (CKIAlpha), an axin associated kinase.13 Monoclonal antibodies reacting specifically with beta-catenin phosphorylated at Ser33 are essential tools for defining the role of the phosphorylated form in the distributions, interactions, and regulation/deregulation of beta-catenin in signal transduction.

General readings

General / background references:Reynolds AB et al. Identification of a new catenin: the tyrosine kinase substrate p120cas associates with E-cadherin complexes. Mol Cell Biol 14:8333-42 (1994). PubMed PMID: 7526156
Tsukita S et al. Submembranous junctional plaque proteins include potential tumor suppressor molecules. J Cell Biol 123:1049-53 (1993). PubMed PMID: 8245116
Johnson KR et al. P- and E-cadherin are in separate complexes in cells expressing both cadherins. Exp Cell Res 207:252-60 (1993). PubMed PMID: 8344378
Edelman GM & Crossin KL Cell adhesion molecules: implications for a molecular histology. Annu Rev Biochem 60:155-90 (1991). PubMed PMID: 1883195
Takeichi M Cadherin cell adhesion receptors as a morphogenetic regulator. Science 251:1451-5 (1991). PubMed PMID: 2006419
Nagafuchi A & Takeichi M Cell binding function of E-cadherin is regulated by the cytoplasmic domain. EMBO J 7:3679-84 (1988). PubMed PMID: 3061804

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Catenin beta-1 (exon 3) (incl. pos. control) antibody

Detection of endogenous beta-Catenin: Whole cell lysates of serum starved tumor cells (20.000 cells/lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab AM00020PU-N (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec).
Lane 1: A431
Lane 2: A549
Lane 3: SKOV3
Lane 4: OVCAR5
Lane 5: HaCaT
Lane 6: PC3
Lane 7: HeLa
Lane 8: HepG2 Mouse IgG1 9G2 Purified Can, Hu, Ms E, ICC/IF, IP, P, WB
0.1 mg / €380.00
  Acris Antibodies GmbH

Catenin beta-1 (C-term) antibody

Formalin fixed, paraffin embedded human stomach tissuem stained with Catenin beta-1 Antibody (C-term) Cat.-No AP51124PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of CTNB1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu ICC/IF, P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Catenin beta-1 antibody

Catenin beta-1 Mouse IgG1 UMAB14 Purified Can, Hu, Mky, Ms, Rt F, WB
0.1 ml / €468.00
  OriGene Technologies, Inc.

UltraMAB

Catenin beta-1 antibody

Immunohistochemical staining of paraffin-embedded human normal lung and lung Adenocarcinoma tissues using anti-beta-catenin mouse monoclonal antibody. (Clone UMAB15, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3mi Mouse IgG1 UMAB15 Purified Hu, Mky, Ms, Rt F, ICC/IF, P, WB
0.1 ml / €468.00
  OriGene Technologies, Inc.

UltraMAB

Catenin beta-1 antibody

Catenin beta-1 Mouse IgG1 UMAB14 Purified Can, Hu, Mky, Ms, Rt F, WB
30 µl / €140.00
  OriGene Technologies, Inc.

UltraMAB

Catenin beta-1 antibody

Immunohistochemical staining of paraffin-embedded human normal lung and lung Adenocarcinoma tissues using anti-beta-catenin mouse monoclonal antibody. (Clone UMAB15, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3mi Mouse IgG1 UMAB15 Purified Hu, Mky, Ms, Rt F, ICC/IF, WB
30 µl / €140.00
  OriGene Technologies, Inc.

UltraMAB

Catenin beta-1 antibody

Western Blot: beta Catenin Antibody (12F7) [TA336809] - analysis of beta Catenin in embryonic lung (lane 1) and embryonic limb (lane 2) lysates using anti-beta Catenin antibody. Each lane was loaded with 5ug of protein sample. Image from verified customer Mouse IgG1 12F7 Purified Chk, Hu, Ms, Prim, Rt C, ICC/IF, IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Catenin beta-1 antibody

Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-CTNNB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500048) Mouse IgG2a OTI1F3 Purified Can, Hu, Mky ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Catenin beta-1 antibody

Catenin beta-1 Mouse IgG2a OTI2H3 Purified Can, Hu, Mky ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Catenin beta-1 antibody

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CTNNB1 monoclonal antibody. Mouse IgG1 OTI2D11 Purified Can, Hu, Mky WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Catenin beta-1 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-CTNNB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502299) Mouse IgG2b OTI1G1 Purified Can, Hu, Mky F, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Catenin beta-1 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-CTNNB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502300) Mouse IgG2b OTI1H10 Purified Hu, Ms P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Catenin beta-1 antibody

Anti-CTNNB1 mouse monoclonal antibody (TA502301) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CTNNB1(RC208947). Mouse IgG2a OTI2F6 Purified Can, Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Catenin beta-1 antibody

Anti-CTNNB1 mouse monoclonal antibody (TA502303) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CTNNB1(RC208947). Mouse IgG2a OTI5D2 Purified Hu, Mky F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Catenin beta-1 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-CTNNB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502304) Mouse IgG2a OTI1E10 Purified Can, Hu F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Catenin beta-1 antibody

Anti-CTNNB1 mouse monoclonal antibody (TA502306) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CTNNB1(RC208947). Mouse IgG1 OTI12C1 Purified Can, Hu, Mky, Rt F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Catenin beta-1 antibody

Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-CTNNB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502337) Mouse IgG2b OTI6A3 Purified Hu, Mky F, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Catenin beta-1 antibody

Anti-CTNNB1 mouse monoclonal antibody (TA502338) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CTNNB1(RC208947). Mouse IgG2a OTI8B5 Purified Hu, Mky, Rt F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Catenin beta-1 antibody

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-CTNNB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502340) Mouse IgG2a OTI1B11 Purified Hu F, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Catenin beta-1 antibody

Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-CTNNB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502341) Mouse IgG2b OTI9H2 Purified Can, Hu, Mky, Rt F, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

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