CATSPER1 antibody

Principal name

CATSPER1 antibody

Alternative names for CATSPER1 antibody

Cation channel sperm-associated protein 1

SwissProt ID

Q8NEC5 (Human), Q91ZR5 (Mouse)

Gene ID

117144 (CATSPER1), 225865 (Catsper1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Rabbit

Available applications

Western blot / Immunoblot (WB), Frozen Sections (C), Paraffin Sections (P)

Background of CATSPER1 antibody

Capacitation is the fil stage of spermatozoon maturation which eventually ebles it to bind the zo pellucida (ZP) - a thick layer of glycoproteins surrounding the oocyte. It occurs in the fallopian tubes of the female reproductive tract and is preceded by spermatogenesis (testes) and epididymal maturation (epididymis). Capacitation is accompanied by drastic morphological and functiol alterations, such as membrane hyperpolarization, changes in membrane lipid composition, intracellular alkalinization, increased level of protein tyrosine phosphorylation by cAMP-dependent PKA, and elevated intracellular concentrations of Ca2+ ([Ca2+]i) and pHi, which render the sperm chemotaxis-responsive and hyperactive. Increased flagellar bend, triggered by Ca2+ sigling, produces asymmetrical, whiplash-like motility pattern that provides the sperm with significantly stronger swimming thrust. Hyperactivity, together with the eruption of hydrolytic enzymes from the sperm's acrosome vesicle facilitate the disruption of the ZP matrix and make way for the sperm to enter the egg cytoplasm. CatSper (cation channel of spermatozoa) is a sperm-specific Ca2+ channel located explicitly in the spermatozoon flagellum2. It mediates flagellar hyperactivity, and is chemotactically responsive to progesterone. CatSper is composed of 4 pore-forming subunits (CatSper1-4) with 6 transmembrane (TM) domains each, and of 3 auxiliary subunits: CatSperÃ? (2 TM domains), CatSper? and CatSperd (1 TM domain each). While all four CatSper proteins are necessary for the channel's function (and hence male fertility)9, each is synthesized independently. Trafficking to the flagellum requires the cooperation of all subunits. CatSper1, the first identified subunit of the channel, is highly homologous to CaV channels; a voltage sensor domain (S1-S4) containing enough positively charged residues to confer weak voltage sensitivity to the channel. Furthermore, its histidine-rich, intracellular amino terminus likely serves as the pH sensor necessary to induce hyperactivation (by permitting an influx of Ca2+). Indeed, intracellular alkaline pH (7.9-8.5) was shown to efficiently hyper-activate CatSper channels.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CATSPER1 antibody

Western blot analysis of rat testis (lanes 1 and 3) and mouse (lanes 2 and 4) lysates: 1. Anti-CatSper1 (extracellular) antibody, (1:200). 2. Anti-CatSper1 (extracellular) antibody, preincubated with the control peptide antigen. Rabbit Purified Ms, Rt WB
0.2 ml / €435.00
  OriGene Technologies, Inc.

CATSPER1 (N-term) antibody

CATSPER1 Antibody (N-term) (Cat. #AP50736PU-N) western blot analysis in HepG2 cell line lysates (35µg/lane).This demonstrates the CATSPER1 antibody detected the CATSPER1 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CATSPER1 antibody

CATSPER1 Rabbit Purified Hu C, P, WB
0.1 ml / €400.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

CATSPER1

CATSPER1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

CATSPER1

CATSPER1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

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