CBFB antibody

Principal name

CBFB antibody

Alternative names for CBFB antibody

Core-binding factor beta, CBF-beta, CBF beta, PEBP2-beta, PEA2-beta, Polyomavirus enhancer-binding protein 2 beta subunit, SL3-3 enhancer factor 1 subunit beta, SL3/AKV core-binding factor beta subunit

SwissProt ID

Q08024 (Mouse), Q13951 (Human)

Gene ID

865 (CBFB)

Available reactivities

Bov (Bovine), Can (Canine), Chk (Chicken), Hu (Human), Ms (Mouse), Rt (Rat), Ze (Zebrafish), African clawed frog

Available hosts

Rabbit, Mouse, Goat

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), ELISA (detection) (E(detection)), ChIP assay (ChIP), Flow Cytometry (F)

Background of CBFB antibody

The protein encoded by this gene is the beta subunit of a heterodimeric core-binding transcription factor belonging to the PEBP2/CBF transcription factor family which master-regulates a host of genes specific to hematopoiesis (e.g., RUNX1) and osteogenesis (e.g., RUNX2). The beta subunit is a non-DNA binding regulatory subunit; it allosterically enhances DNA binding by alpha subunit as the complex binds to the core site of various enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers and GM-CSF promoters. Alternative splicing generates two mRNA variants, each encoding a distinct carboxyl terminus. In some cases, a pericentric inversion of chromosome 16 [inv(16)(p13q22)] produces a chimeric transcript consisting of the N terminus of core-binding factor beta in a fusion with the C-terminal portion of the smooth muscle myosin heavy chain 11. This chromosomal rearrangement is associated with acute myeloid leukemia of the M4Eo subtype. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]. Transcript Variant: This variant (2) uses an alternate splice site in the 3' coding region compared to variant 1, that causes a frameshift. The resulting isoform (2) is shorter and has a distinct C-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

Speck NA., et al. (2009) Blood. 113(13):3070-9.
Ito Y., et al. (2008) Blood. 112(9):3777-87.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CBFB antibody

Western blot analysis of extracts from HeLa cells and HUVEC cells, using CBF ß antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CBFB antibody

Western blot analysis of extracts from HepG2 cells, COLO cells, Jurkat cells, HUVEC cells and COS cells, using PPP4R1L antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CBFB antibody

Anti-CBFB (0.3ug/ml) staining of Daudi lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CBFB antibody

CBFB Antibody (Center) (Cat. #TA324878) western blot analysis in K562 cell line and mouse lung tissue lysates (35ug/lane).This demonstrates the CBFB antibody detected the CBFB protein (arrow). Rabbit Hu WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

CBFB antibody

WB Suggested Anti-CBFB Antibody Titration: 0.2-1 ug/ml; Positive Control: K562 cell lysate Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

CBFB antibody

Western blot analysis of extracts of various cell lines, using CBFB antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

CBFB antibody

ChIP assays were performed using SKNO-1 cells, the antibody against CBFb and optimized primer pairs for qPCR. Sheared chromatin from 1.25 million cells and 4 ul of antibody were used per ChIP experiment. QPCR was performed using primers specific for the FUT7, OGG1, NFE2, and SPI1 genes. Image shows the relative occupancy, calculated as the ratio + control/background for which the MYOG gene was used. Rabbit IgG Purified Hu E
0.1 ml / €325.00
  OriGene Technologies, Inc.

CBFB antibody

Immunohistochemistry (IHC) analyzes of PEBP2beta antibody (Cat.-No.: AP202442PU-N) in paraffin-embedded human heart tissue. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

CBFB antibody

CBFB polyclonal antibody (A01), Lot # 050928JC01 Western Blot analysis of CBFB expression in Jurkat ( Cat # L017V1 ). Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

CBFB antibody

CBFB Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

CBFB antibody

Immunohistochemistry analysis of paraffin-embedded human heart tissue, using CBF beta Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
50 µg / €205.00
  Assay Biotech

CBFB antibody

Immunohistochemistry analysis of paraffin-embedded human heart tissue, using CBF beta Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €310.00
  Assay Biotech

CBFB antibody

Human K562; WB Suggested Anti-CBFB Antibody Titration: 0.2-1 ug/ml. Positive Control: K562 cell lysate; CBFB antibody - N-terminal region (P100598_P050) in Human K562 cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

CBFB antibody

Immunohistochemistry: PEBP2beta Antibody [NBP1-19597] -  PEBP2beta Antibody [NBP1-19597] - paraffin-embedded human heart tissue. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €420.00
  Novus Biologicals Inc.

CBFB antibody

CBFB Rabbit IgG Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €360.00
  Novus Biologicals Inc.

CBFB antibody

CBFB Rabbit IgG Aff - Purified Hu, Ms, Rt E, P, WB
50 µg / €290.00
  Novus Biologicals Inc.

CBFB antibody

Immunohistochemistry-Paraffin: CBFb Antibody [NBP1-87300] - Immunohistochemical staining of human colon shows moderate membranous positivity in glandular cells. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

CBFB (transcript variant 2)

CBFB Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

CBFB (1-182, His-tag)

CBFB Human Purified > 85 % by SDS-PAGE E. coli
0.25 mg / €750.00
  Acris Antibodies GmbH

CBFB (1-182, His-tag)

CBFB Human Purified > 85 % by SDS-PAGE E. coli
50 µg / €300.00
  Acris Antibodies GmbH

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