CBLC / RNF57 antibody

Principal name

CBLC / RNF57 antibody

Alternative names for CBLC / RNF57 antibody

CBL3, CBL-C, CBL-3, RING finger protein 57, Signal transduction protein CBL-C, SH3-binding protein CBL-C

SwissProt ID

Q80XL1 (Mouse), Q9ULV8 (Human)

Gene ID

23624 (CBLC)

Available reactivities

Hu (Human), Chimp (Chimpanzee), Ms (Mouse), Rt (Rat)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Flow Cytometry (F)

Background of CBLC / RNF57 antibody

Cbl-c is also known as signal transduction protein CBL-C, SH3-binding protein CBL-C, CBL-3, and RING finger protein 57. Cbl proteins are a family of ubiquitin protein ligases (E3s) that negatively regulate signaling by targeting activated tyrosine kinases for degradation. Cbl-c (a.k.a. Cbl-3) is the most recently cloned member of the Cbl proteins and is expressed only in epithelial cells (the other Cbl proteins are ubiquitously expressed). Cbl-c, like the other mammalian Cbl proteins, can ubiquitinate the activated EGFR and target it for degradation. Cbl-c knock out mice show no obvious phenotype. Thus, the physiological role of Cbl-c is not known.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CBLC / RNF57 (444-458) antibody

IP and WB using Anti-Cbl-c antibody shows detection of a pre-dominant band at ~52 kDa corresponding to Cbl-c (arrowhead) in transfected cell lysates (left panel). Lysates are from Hek 293T cells transfected with empty vector or with Cbl-c. The predicted size of Cbl-c is 52 kDa. Size markers in kDa are shown to the left of the panel. The right panel shows WBting after first immunoprecipitating with Rabbit anti-Cbl-c followed by WBting using a Goat anti-Cbl-c antibody. Rabbit IgG Aff - Purified Chimp, Hu E, IP, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CBLC / RNF57 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CBLC (RC205130, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CBLC. Mouse IgG1 OTI3D9 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CBLC / RNF57 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CBLC (RC205130, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CBLC. Mouse IgG2b OTI3B4 Purified Hu F, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

CBLC / RNF57 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CBLC (RC205130, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CBLC. Mouse IgG1 OTI4E3 Purified Hu F, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CBLC / RNF57 antibody

Pancreas: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Aff - Purified Hu E, ICC/IF, IP, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

CBLC / RNF57 (N-term) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CBLC / RNF57 (C-term) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CBLC / RNF57 antibody

CBLC / RNF57 Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

CBLC / RNF57 antibody

CBLC / RNF57 Mouse IgG2a 3F10 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

CBLC / RNF57 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with CBLC polyclonal antibody ( Cat # PAB1697 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma. Rabbit Hu E, P, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

CBLC / RNF57 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with CBLC polyclonal antibody ( Cat # PAB1698 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma. Rabbit Hu E, P, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

CBLC / RNF57 antibody

Immunohistochemistry of CBLC polyclonal antibody ( Cat # PAB9985 ) was used at 5 µg/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides.
This image shows moderate intracellular positive staining of human pancreatic acinar epithelium at 40X.
Tissue was formalin-fixed and paraffin embedded.
The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
Personal Communi-cation, Tina Roush, Life Span Biosciences, Seattle, WA. Chimp, Hu, Ms, Rt
0.1 mg / €560.00
  Abnova Taiwan Corp.

CBLC / RNF57 antibody

CBLC / RNF57 Rabbit Aff - Purified Hu E, ICC/IF, IP, P, WB
50 µg / €480.00
  Novus Biologicals Inc.

CBLC / RNF57 antibody

Western Blot: FBX09 Antibody [NBP1-78064] shows detection of a band at ~100 kDa (arrowhead) believed to correspond to FBOX9 present in a MCF7 whole cell lysate (lane 1). Specific band reactivity is greatly diminished when the antibody is pre-incubated with the immunizing peptide (data not shown). Approximately 35 ug of lysate was separated by 4-20% Tris Glycine SDS-PAGE. After blocking, the membrane was probed overnight at 4C with the primary antibody diluted to 1:1,500. The membrane was washed and reacted with a 1:10,000 dilution of Dye 800 conjugated Gt-a-Rabbit IgG [H&L] for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers (indicated at left, 700 nm channel, red). Rabbit Aff - Purified Hu E, WB
0.1 mg / €510.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

CBLC / RNF57 (transcript variant 1)

CBLC / RNF57 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

CBLC / RNF57

CBLC / RNF57 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

CBLC / RNF57

CBLC / RNF57 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

CBLC overexpression lysate

CBLC overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Lysates (Overexpressed)

Catalog No.    

CBLC overexpression lysate

CBLC overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

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