CBX3 antibody

Principal name

CBX3 antibody

Alternative names for CBX3 antibody

Chromobox protein homolog 3, HECH, HP1 gamma, HP1-gamma, HP1Hs-gamma, Heterochromatin protein 1 homolog gamma, Modifier 2 protein

SwissProt ID

P23198 (Mouse), Q13185 (Human)

Gene ID

11335 (CBX3), 3113359 (argH)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Bov (Bovine), Chk (Chicken), Dros (Drosophila), African clawed frog, Por (Porcine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit), Hst (Hamster)

Available hosts

Rabbit, Goat, Rat, Mouse

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Paraffin Sections (P), Western blot / Immunoblot (WB), Frozen Sections (C), PLA/Proximity ligation assay (PLA), Dot blot (Dot), ChIP assay (ChIP), Immunofluorescence (IF)

Background of CBX3 antibody

At the nuclear envelope, the nuclear lamina and heterochromatin are adjacent to the inner nuclear membrane. CBX3 binds DNA and is a component of heterochromatin. CBX3 also can bind lamin B receptor, an integral membrane protein found in the inner nuclear membrane. The dual binding functions of CBX3 may explain the association of heterochromatin with the inner nuclear membrane.At the nuclear envelope, the nuclear lamina and heterochromatin are adjacent to the inner nuclear membrane. The protein encoded by this gene binds DNA and is a component of heterochromatin. This protein also can bind lamin B receptor, an integral membrane protein found in the inner nuclear membrane. The dual binding functions of the encoded protein may explain the association of heterochromatin with the inner nuclear membrane. Two transcript variants encoding the same protein but differing in the 5\' UTR, have been found for this gene.At the nuclear envelope, the nuclear lamina and heterochromatin are adjacent to the inner nuclear membrane. The protein encoded by this gene binds DNA and is a component of heterochromatin. This protein also can bind lamin B receptor, an integral membrane protein found in the inner nuclear membrane. The dual binding functions of the encoded protein may explain the association of heterochromatin with the inner nuclear membrane. Two transcript variants encoding the same protein but differing in the 5\' UTR, have been found for this gene.

General readings

Vakoc CR, Mandat SA, Olenchock BA, Blobel GA. Histone H3 Lysine 9 Methylation and HP1gamma Are Associated with Transcription Elongation through Mammalian Chromatin. Mol Cell. 2005 Aug 5;19(3):381-391. PMID: 16061184

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CBX3 antibody

Western blot detection of HP1-gamma in Hela,3T3,C6,COS7 and CHO-K1 cell lysates using HP1-gamma mouse mAb (1:1000 diluted).Predicted band size:22KDa.Observed band size:22KDa. Mouse IgG1 5G10-F7-A12 Purified Hst ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CBX3 antibody

TA302653 (0.3µg/ml) staining of A431 lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt P, WB
20 µg / €200.00
  Acris Antibodies GmbH

CBX3 antibody

TA302653 (0.3µg/ml) staining of A431 lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CBX3 antibody

Western blot analysis of extracts from 293 cells, using HP1? (Ab-93) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CBX3 antibody

WB Suggested Anti-CBX3 Antibody Titration: 1.25 ug/ml; Positive Control: Jurkat cell lysate CBX3 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CBX3 antibody

WB Suggested Anti-CBX3 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:12500; Positive Control: Jurkat cell lysate CBX3 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

CBX3 antibody

Determination of the antibody titer To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody against human CBX3. The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:3,250. Rabbit IgG Purified Hu E
0.1 ml / €325.00
  OriGene Technologies, Inc.

CBX3 antibody

Immunohistochemistry on paraffin sections: Human brain cortex, stained with AM26741PU-N. Mouse IgG1 2MOD-1G6 Purified Hu, Ms, Por P
0.1 mg / €440.00
  Acris Antibodies GmbH

CBX3 (74-87) antibody

Figure 1. Immunohistochemical analysis of Formalin-Fixed Paraffin-Embedded Human Placenta tissue using AP08786PU-N at 2.5 µg/ml. Goat IgG Aff - Purified Bear, Bov, Bt, Can, Chk, Eq, Frog, Hst, Hu, L, Mky, Ms, Op, Por, Prim, Rb, Rt, Tr E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

CBX3 pSer93 antibody

Western blot analysis of extracts from K562 cells treated with forskolin using HP1γ (Phospho-Ser93) Antibody AP55787PU-N.The lane on the right is treated with the antigen-specific peptide. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 ml / €340.00
  Acris Antibodies GmbH

CBX3 pSer93 antibody

Western blot analysis of extracts from K562 cells treated with forskolin using HP1γ (Phospho-Ser93) Antibody AP55787PU-N.The lane on the right is treated with the antigen-specific peptide. Rabbit Aff - Purified Hu, Ms, Rt WB
50 µl / €270.00
  Acris Antibodies GmbH

CBX3 antibody

CBX3 Rat IgM MAC385 Supernatant Hu, Ms C, E, WB
2 ml / €410.00
  Acris Antibodies GmbH

CBX3 antibody

CBX3 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

CBX3 antibody

Immunofluorescence of monoclonal antibody to CBX3 on HeLa cell. [antibody concentration 10 ug/ml] Mouse IgG2a 1G12-1D9 Purified Hu E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

CBX3 antibody

CBX3 Mouse IgG2a S3 Ascites Hu E, WB
0.2 ml / €370.00
  Abnova Taiwan Corp.

CBX3 antibody

CBX3 polyclonal antibody ( Cat # PAB6885 ) ( 2.5µg/ml ) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Goat Hu, Ms E, P, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

CBX3 pSer93 antibody

Western blot analysis of extracts from K562 cells treated with forskolin 40nM 30', using HP1 gamma (Phospho-Ser93) Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €205.00
  Assay Biotech

CBX3 pSer93 antibody

Western blot analysis of extracts from K562 cells treated with forskolin 40nM 30', using HP1 gamma (Phospho-Ser93) Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €310.00
  Assay Biotech

CBX3 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using HP1 gamma (Ab-93) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
50 µg / €205.00
  Assay Biotech

CBX3 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using HP1 gamma (Ab-93) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €310.00
  Assay Biotech

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