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CBX5 antibody

Principal name

CBX5 antibody

Alternative names for CBX5 antibody

Chromobox protein homolog 5, HP1A, p25, HP1 alpha, HP1-alpha, Heterochromatin protein 1 homolog alpha

SwissProt ID

P45973 (Human), Q61686 (Mouse)

Gene ID

23468 (CBX5), 12419 (Cbx5)

Ncbi ID

NP_001120793.1, NP_036249, 9606, NP_001120794.1, NP_001120793, NP_001120794, NM_007626, NM_001127322, NM_001127321, NM_001110216, NM_012117

Available reactivities

Hu (Human), Ms (Mouse), Bt (Bat), Bov (Bovine), Can (Canine), Eq (Equine), Hst (Hamster), Mky (Monkey), Por (Porcine), Prim (Primate), Rb (Rabbit), Rt (Rat), Sh (Sheep), Bear

Available hosts

Rabbit, Goat, Mouse

Available applications

Enzyme Immunoassay (E), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), EPair for Elisa (EPair), ELISA (detection) (E(detection)), Immunofluorescence (IF), Immunoprecipitation (IP)

Background of CBX5 antibody

Heterochromatin protein-1 (HP1) is a methyl-lysine binding protein localized at heterochromatin sites, where it mediates gene silencing. It has been shown that mammalian methyltransferases that selectively methylate histone H3 on lysine-9 generate a binding site for HP1 proteins, a family of heterochromatic adaptor molecules implicated in both gene silencing and supranucleosomal chromatin structure. High-affinity in vitro recognition of a methylated histone H3 peptide by HP1 requires a functiol chromodomain. Thus, the HP1 chromodomain is a specific interaction motif for the methyl epitope on lysine-9 of histone H3. In vivo, heterochromatin association of HP1 proteins is lost in Suv39h double- primary mouse fibroblasts but is restored after reintroduction of a catalytically active SUV39H1 HMTase. A molecular mechanism through which the SUV39H-HP1 methylation system can contribute to the propagation of heterochromatic subdomains in tive chromatin has been defined. It has been demonstrated that HP1 can bind with high affinity to histone H3 methylated at lysine-9 but not at lysine-4. The chromodomain of HP1 as its methyl-lysine-binding domain has been identified. A point mutation in the chromodomain, which destroys the gene silencing activity of HP1 in Drosophila, abolished methyl-lysine-binding activity. Genetic and biochemical alysis in S. pombe showed that the methylase activity of Clr4 (the SUV39H1 homolog) is necessary for the correct localization of Swi6 (the HP1 equivalent) at centromeric heterochromatin and for gene silencing. A stepwise model for the formation of a transcriptiolly silent heterochromatin: SUV39H1 places a methyl marker on histone H3, which is then recognized by HP1 through its chromodomain has been suggested. This model may also explain the stable inheritance of the heterochromatic state. SUV39H1 and HP1 are both involved in the repressive functions of the retinoblastoma protein. Rb associates with SUV39H1 and HP1 in vivo by mean

General readings

Rohr O, Lecestre D, Chasserot-Golaz S, Marban C, Avram D, Aunis D, Leid M, Schaeffer E. Recruitment of Tat to heterochromatin protein HP1 via interaction with CTIP2 inhibits human immunodeficiency virus type 1 replication in microglial cells. J Virol. 2003 May;77(9):5415-27.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using HP1 alpha (Phospho-Ser92) Antibody. The picture on the right is treated with the synthesized peptide.

Immunohistochemistry (IHC) analyzes of HP1-alpha antibody (Cat.-No.: AP06694PU-N) in paraffin-embedded human liver carcinoma tissue.

AP06694PU-N

Western blot (WB) analysis of CBX5 antibody (Cat.-No.. AP06694PU-N) at 1/500 dilution
Lane1:HEK293T whole cell lysate
Lane2:Mouse spleen tissue lysate
Lane3:Rat spleen tissue lysate

AP06694PU-N

Figure 1. Immunohistochemical analysis of Formalin-Fixed Paraffin-Embedded Human Uterus tissue using AP08784PU-N at 2.5 µg/ml.

AP08784PU-N

Figure 2. Antibody (AP08784PU-N at 0.03 µg/ml) staining of Hela lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by Chemiluminescence.

AP08784PU-N

Human NT-2; . Sample Type :. Human NT-2 cells . Primary Antibody Dilution :. 1:500. Secondary Antibody :. Goat anti-rabbit Alexa-Fluor 594. Secondary Antibody Dilution :. 1:1000. Color/Signal Descriptions :. CBX5: Red DAPI:Blue. Gene Name :. CBX5. Submitted by :. Dr. Yuzhi Chen, University of Arkansas for Medical Science; CBX5 antibody - middle region (ARP32481_T100) in Human NT-2 cells using Immunohistochemistry

ARP32481_T100

Human brain; WB Suggested Anti-CBX5 Antibody Titration: 1.25ug/ml. Positive Control: Human brain; CBX5 antibody - middle region (ARP32481_T100) in Human brain cells using Western Blot

ARP32481_T100

Human NT-2; . Sample Type: 1. Human NT-2 cells (60ug). Primary Antibody Dilution:Â 2ug/ml. Secondary Antibody: IRDye 800CW goat anti-rabbit from Li-COR Bioscience. Secondary Antibody Dilution:Â 1: 20,000. Image Submitted by: Yuzhi Chen. University of Arkansas for Medical ScienceÂ .; CBX5 antibody - middle region (ARP32481_T100) in Human NT-2 cells using Western Blot

ARP32481_T100

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using HP1 alpha (Ab-92) Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from Jurkat cells, treated with insulin 0.01U/ml 15', using HP1 alpha (Ab-92) Antibody. The lane on the right is treated with the synthesized peptide.

Western blot analysis of extracts from HeLa/A549/K562 cells, using CBX5 Antibody. The lane on the right is treated with the synthesized peptide.

Immunofluorescence of purified MaxPab antibody to CBX5 on HeLa cell. [antibody concentration 10 ug/ml]

H00023468-B01

Western Blot analysis of CBX5 expression in transfected 293T cell line (H00023468-T01) by CBX5 MaxPab polyclonal antibody.Lane 1: CBX5 transfected lysate(21.01 KDa).Lane 2: Non-transfected lysate.

H00023468-B01

CBX5 MaxPab polyclonal antibody. Western Blot analysis of CBX5 expression in C32.

H00023468-B01

Immunofluorescence of purified MaxPab rabbit antibody to CBX5 on HeLa cell. [antibody concentration 10 ug/ml]

H00023468-D01P

Western Blot analysis of CBX5 expression in transfected 293T cell line (H00023468-T01) by CBX5 MaxPab polyclonal antibody.Lane 1: CBX5 transfected lysate(22.20 KDa).Lane 2: Non-transfected lysate.

H00023468-D01P

CBX5 MaxPab rabbit polyclonal antibody. Western Blot analysis of CBX5 expression in Jurkat.

H00023468-D01P

Immunofluorescence of monoclonal antibody to CBX5 on HeLa cell. [antibody concentration 10 ug/ml]

H00023468-M01

Western Blot analysis of CBX5 expression in transfected 293T cell line by CBX5 monoclonal antibody (M01), clone 1E11-3A10.Lane 1: CBX5 transfected lysate(22.2 KDa).Lane 2: Non-transfected lysate.

H00023468-M01

CBX5 monoclonal antibody (M01), clone 1E11-3A10 Western Blot analysis of CBX5 expression in HeLa NE ( Cat # L013V3 ).

H00023468-M01

Detection limit for recombinant GST tagged CBX5 is approximately 0.03ng/ml as a capture antibody.

H00023468-M01

Western blot analysis of CBX5 in HeLa whole cell lysate using CBX5 polyclonal antibody ( Cat # PAB12465 ).

PAB12465

CBX5 polyclonal antibody ( Cat # PAB6883 ) ( 2.5µg/ml ) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

PAB6883

CBX5 polyclonal antibody ( Cat # PAB6883 ) ( 0.03 µg/ml ) staining of Hela lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.

PAB6883

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HP1α (Phospho-Ser92) antibody AP55795PU-N (left)or the same antibody preincubated with blocking peptide (right).

Immunohistochemical analysis of HP1 alpha staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of HP1 alpha staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Western blot analysis of HP1 alpha expression in HeLa (A); NIH3T3 (B); C6 (C) whole cell lysates.

TP301515

TP325208

TP760487

Detection of HP 1 alpha in HeLa whole cell lysate. 1 min. ECL exposure

TA301513

TA302445 (0.03µg/ml) staining of Hela lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

TA302445

Western blot analysis of extracts from Jurkat cells, treated with insulin (0.01U/ml, 15mins), using HP1 alpha (Ab-92) antibody.The lane on the right is treated with the synthesized peptide.

TA312347

Immunohistochemistry analysis of paraffin-embedded human braina tissue using HP1 alpha (Ab-92) antibody.The picture on the right is treated with the synthesized peptide.

TA312347

Predicted band size: 22kDa. Positive control: Hela cell lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lysate: 24 ug Primary antibody: 1/200 dilution Secondary antibody: Donkey anti Rabbit IgG - H&L (HRP) at 1/3000 dilution Exposure time: 1 minute)

TA323817

Predicted band size: 22kDa. Positive control: Hela cell and Human endometrial cancer tissue lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lane1: Hela cell lysate Lane2: Human endometrial cancer tissue lysate Lysates: 24 ug per lane Primary antibody: 1/300 dilution Secondary antibody: Donkey anti Rabbit IgG - H&L (HRP) at 1/3000 dilution Exposure time: 1 minute)

TA323818

Western blot analysis of extracts from HUVEC cells, using CBX5 antibody.The lane on the right is treated with the synthesized peptide.

TA326263

Western blot analysis of extracts of various cell lines, using CBX5 antibody.

TA326926

Immunohistochemistry of paraffin-embedded human lung cancer using CBX5 antibody at dilution of 1:200 (400x lens).

TA326926

Immunohistochemistry of paraffin-embedded human stomach cancer using CBX5 antibody at dilution of 1:200 (400x lens).

TA326926

Immunofluorescence analysis of HeLa cell using CBX5 antibody. Blue: DAPI for nuclear staining.

TA326926

WB Suggested Anti-CBX5 Antibody Titration: 1.25ug/ml; Positive Control: Human brain

TA330563

Sample Type: 1. Human NT-2 cells (60ug); Primary Antibody Dilution:ï¿½2ug/ml; Secondary Antibody: IRDye 800CW goat anti-rabbit from Li-COR Bioscience; Secondary Antibody Dilution:ï¿½1: 20,000; Image Submitted by: Yuzhi Chen; University of Arkansas for Med

TA330563

Sample Type : Human NT-2 cells Primary Antibody Dilution : 1:500Secondary Antibody : Goat anti-rabbit Alexa-Fluor 594Secondary Antibody Dilution : 1:1000Color/Signal Descriptions : CBX5: Red DAPI:BlueGene Name : CBX5Submitted by : Dr. Yuzhi Chen, Universi

TA330563

Western blot detection of HP1 alpha in MCF7,293T,HelaNE,3T3,C6,COS7 and Mouse brain lysates and using HP1 alpha mouse mAb (1:1000 diluted).Predicted band size: 22KDa.Observed band size: 22KDa

TA346905

Immunohistochemical analysis of paraffin-embedded human breast carcinoma with HP1 alpha mouse mAb (3G2-H10-A6, 1:400 diluted ),showing nuclear localization.A high pressure mediated antigen retrieval step was performed in citrat buffer(pH6.0).

TA346905

Immunocytochemistry stain of Hela using HP1 alpha mouse mAb (1:300).

TA346905

WB was performed on nuclear extracts from HeLa cells (20 ug) with the antibody against human HP1a, diluted 1:1,000 in TBS-Tween containing 5% skimmed milk (Figure 1). The molecular weight marker (in kDa) is shown on the left; the expected location of HP1a, HP1ß and HP1? is indicated on the right. The WB analysis clearly demonstrates that the HP1a antibody specifically recognizes HP1a and not HP1ß and HP1?.

TA347090

HeLa cells were stained with the antibody against HP1aand with DAPI. Cells were fixed with 4% formaldehyde for 10' and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the HP1a antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.

TA347090

WB was performed on nuclear extracts from HeLa cells (20 ug) with the antibody against human HP1a, ß and ? diluted 1:1,000 in TBS-Tween containing 5% skimmed milk (Figure 1). The molecular weight marker (in kDa) is shown on the left; the expected location of HP1a, HP1ß and HP1? is indicated on the right.

TA347091

ChIP assays were performed using NIH3T3 cells and 4 ug of the antibody against HP1a, ß and ?. QPCR was performed on the IP’d DNA with optimized primer sets for the rDNA promoter and for a subtelomeric sequence of chromosome 19. Image shows the relative enrichment as compared to a no antibody negative control ChIP.

TA347091

HeLa cells were stained with the antibody against HP1a,ß,? and with DAPI. Cells were fixed with 4% formaldehyde for 10' and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the HP1a,ß,? antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.

TA347091

WB was performed on nuclear extracts from HeLa cells (HeLa NE, 20 ug) using the antibody against hHP1 alpha diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. A molecular weight marker (in kDa) is shown on the left. The location of the three HP1 isoforms of interest is indicated on the right, demonstrating that the antibody only recognizes the alpha isoform.

TA347092

WB was performed on nuclear extracts from HeLa cells (HeLa NE, 20 ug) using the antibody against HP1 alpha, beta and gamma diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. A molecular weight marker (in kDa) is shown on the left. The location of the three HP1 isoforms is indicated on the right.

TA347093

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using HP1a (Phospho-Ser92) antibody.The picture on the right is treated with the synthesized peptide.

TA312348

Primary Antibodies

Catalog No.	Host	Iso.	Clone	Pres.	React.	Applications
TA326926	CBX5 antibody
	Rabbit anti-CBX5 Polyclonal Antibody
	Rabbit	IgG		Purified	Hu, Ms, Rt		0.1 mg / €335.00
							OriGene Technologies, Inc.
+3 additional images	Highly validated antibody!
TA301513	CBX5 antibody
	Rabbit Polyclonal Antibody against HP 1 alpha
	Rabbit	IgG		Purified	Hu	WB	0.1 ml / €416.00
							OriGene Technologies, Inc.
TA302445	CBX5 antibody
	Goat Polyclonal Antibody against CBX5 / HP1-Alpha
	Goat			Aff - Purified	Can, Hu, Ms	P, WB	0.1 mg / €360.00
							OriGene Technologies, Inc.
TA312347	CBX5 antibody
	Rabbit polyclonal HP1 alpha (Ab-92) antibody
	Rabbit	IgG		Purified	Hu	ICC/IF, P, WB	0.1 ml / €335.00
							OriGene Technologies, Inc.
+1 additional image
TA312348	CBX5 antibody
	Rabbit polyclonal HP1a (Ser92) antibody(Phospho-specific)
	Rabbit	IgG		Purified	Hu	E, P	0.1 ml / €335.00
							OriGene Technologies, Inc.
TA323817	CBX5 antibody
	Anti-CBX5 Rabbit Polyclonal Antibody
	Rabbit	IgG		Purified	Hu, Ms, Rt	E, WB	0.1 ml / €335.00
							OriGene Technologies, Inc.
TA323818	CBX5 antibody
	Anti-CBX5 Rabbit Polyclonal Antibody
	Rabbit	IgG		Purified	Hu, Ms, Rt	E, WB	0.1 ml / €335.00
							OriGene Technologies, Inc.
TA326263	CBX5 antibody
	Rabbit polyclonal CBX5 antibody
	Rabbit	IgG		Purified	Hu, Ms	WB	0.1 ml / €335.00
							OriGene Technologies, Inc.
TA330563	CBX5 antibody
	Rabbit Polyclonal Anti-CBX5 Antibody
	Rabbit	IgG		Purified	Bov, Can, Eq, Hu, Ms, Por, Rb, Rt, Sh	ICC/IF, WB	0.1 mg / €360.00
							OriGene Technologies, Inc.
+2 additional images
TA346905	CBX5 antibody
	Mouse Monoclonal HP1 alpha Antibody
	Mouse	IgG2b	3G2-H10-A6	Purified		ICC/IF, WB	0.1 ml / €335.00
							OriGene Technologies, Inc.
+2 additional images
TA347090	CBX5 antibody
	Rabbit Polyclonal HP1alpha Antibody
	Rabbit	IgG		Purified	Hu		50 µg / €392.00
							OriGene Technologies, Inc.
+1 additional image
TA347091	CBX5 antibody
	Rabbit Polyclonal HP1alpha/beta/gamma Antibody
	Rabbit	IgG		Purified	Hu, Ms		50 µg / €392.00
							OriGene Technologies, Inc.
+2 additional images
TA347092	CBX5 antibody
	Rabbit Polyclonal HP1 alpha Antibody
	Rabbit	IgG		Purified	Hu		0.1 ml / €335.00
							OriGene Technologies, Inc.
TA347093	CBX5 antibody
	Rabbit Polyclonal HP1 alpha, beta and gamma Antibody
	Rabbit	IgG		Purified	Hu		0.1 ml / €335.00
							OriGene Technologies, Inc.
AP06694PU-N	CBX5 antibody
	Rabbit			Aff - Purified	Hu, Ms	ICC/IF, P, WB	0.1 mg / €350.00
							Acris Antibodies GmbH
+1 additional image
AP08784PU-N	CBX5 (88-102) antibody
	Goat	IgG		Aff - Purified	Bov, Elephant, Eq, Hu, Mky, Por, Sh	E, P, WB	50 µg / €380.00
							Acris Antibodies GmbH
+1 additional image
AP55795PU-N	CBX5 pSer92 antibody
	Rabbit			Aff - Purified	Hu	P	0.1 mg / €340.00
							Acris Antibodies GmbH
AP55795PU-S	CBX5 pSer92 antibody
	Rabbit			Aff - Purified	Hu	P	50 µg / €270.00
							Acris Antibodies GmbH
A8262-1	CBX5 pSer92 antibody
	Rabbit			Aff - Purified	Hu	E, P	50 µg / €230.00
							Assay Biotech
A8262-2	CBX5 pSer92 antibody
	Rabbit			Aff - Purified	Hu	E, P	0.1 mg / €330.00
							Assay Biotech