CBX5 antibody

Principal name

CBX5 antibody

Alternative names for CBX5 antibody

Chromobox protein homolog 5, HP1A, p25, HP1 alpha, HP1-alpha, Heterochromatin protein 1 homolog alpha

SwissProt ID

P45973 (Human), Q61686 (Mouse)

Gene ID

23468 (CBX5), 4023468 (IPR000819)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Ms (Mouse), Rt (Rat), Hst (Hamster), Eq (Equine), Por (Porcine), Rb (Rabbit), Sh (Sheep)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Paraffin Sections (P), PLA/Proximity ligation assay (PLA), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Flow Cytometry (F), Frozen Sections (C), Immunofluorescence (IF), ChIP assay (ChIP)

Background of CBX5 antibody

Heterochromatin protein-1 (HP1) is a methyl-lysine binding protein localized at heterochromatin sites, where it mediates gene silencing. It has been shown that mammalian methyltransferases that selectively methylate histone H3 on lysine-9 generate a binding site for HP1 proteins, a family of heterochromatic adaptor molecules implicated in both gene silencing and supranucleosomal chromatin structure. High-affinity in vitro recognition of a methylated histone H3 peptide by HP1 requires a functional chromodomain. Thus, the HP1 chromodomain is a specific interaction motif for the methyl epitope on lysine-9 of histone H3. In vivo, heterochromatin association of HP1 proteins is lost in Suv39h double-null primary mouse fibroblasts but is restored after reintroduction of a catalytically active SUV39H1 HMTase. A molecular mechanism through which the SUV39H-HP1 methylation system can contribute to the propagation of heterochromatic subdomains in native chromatin has been defined. It has been demonstrated that HP1 can bind with high affinity to histone H3 methylated at lysine-9 but not at lysine-4. The chromodomain of HP1 as its methyl-lysine-binding domain has been identified. A point mutation in the chromodomain, which destroys the gene silencing activity of HP1 in Drosophila, abolished methyl-lysine-binding activity. Genetic and biochemical analysis in S. pombe showed that the methylase activity of Clr4 (the SUV39H1 homolog) is necessary for the correct localization of Swi6 (the HP1 equivalent) at centromeric heterochromatin and for gene silencing. A stepwise model for the formation of a transcriptionally silent heterochromatin: SUV39H1 places a methyl marker on histone H3, which is then recognized by HP1 through its chromodomain has been suggested. This model may also explain the stable inheritance of the heterochromatic state. SUV39H1 and HP1 are both involved in the repressive functions of the retinoblastoma protein. Rb associates with SUV39H1 and HP1 in vivo by means of its pocket domain. SUV39H1 cooperates with Rb to repress the cyclin E promoter, and in fibroblasts that are disrupted for SUV39H1, the activity of the cyclin E and cyclin A2 genes are specifically elevated. The SUV39H1-HP1 complex is not only involved in heterochromatic silencing but also has a role in repression of euchromatic genes by Rb and perhaps other corepressor proteins.

General readings

Rohr O, Lecestre D, Chasserot-Golaz S, Marban C, Avram D, Aunis D, Leid M, Schaeffer E. Recruitment of Tat to heterochromatin protein HP1 via interaction with CTIP2 inhibits human immunodeficiency virus type 1 replication in microglial cells. J Virol. 2003 May;77(9):5415-27. PMID: 12692243

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CBX5 antibody

Western blot analysis of extracts of various cell lines, using CBX5 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

CBX5 antibody

Immunofluorescence of monoclonal antibody to CBX5 on HeLa cell. [antibody concentration 10 ug/ml] Mouse IgG1 1E11-3A10 Purified Hu E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

CBX5 antibody

Detection of HP 1 alpha in HeLa whole cell lysate. 1 min. ECL exposure Rabbit IgG Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CBX5 antibody

TA302445 (0.03µg/ml) staining of Hela lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CBX5 antibody

Western blot analysis of extracts from Jurkat cells, treated with insulin (0.01U/ml, 15mins), using HP1 alpha (Ab-92) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CBX5 antibody

Predicted band size: 22kDa. Positive control: Hela cell lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lysate: 24 ug Primary antibody: 1/200 dilution Secondary antibody: Donkey anti Rabbit IgG - H&L (HRP) at 1/3000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CBX5 antibody

Predicted band size: 22kDa. Positive control: Hela cell and Human endometrial cancer tissue lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lane1: Hela cell lysate Lane2: Human endometrial cancer tissue lysate Lysates: 24 ug per lane Primary antibody: 1/300 dilution Secondary antibody: Donkey anti Rabbit IgG - H&L (HRP) at 1/3000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CBX5 antibody

Western blot analysis of extracts from HUVEC cells, using CBX5 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CBX5 antibody

WB Suggested Anti-CBX5 Antibody Titration: 1.25ug/ml; Positive Control: Human brain Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Por, Rb, Rt, Sh ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CBX5 antibody

Western blot detection of HP1 alpha in MCF7,293T,HelaNE,3T3,C6,COS7 and Mouse brain lysates and using HP1 alpha mouse mAb (1:1000 diluted).Predicted band size: 22KDa.Observed band size: 22KDa Mouse IgG2b 3G2-H10-A6 Purified ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CBX5 antibody

WB was performed on nuclear extracts from HeLa cells (20 ug) with the antibody against human HP1a, diluted 1:1,000 in TBS-Tween containing 5% skimmed milk (Figure 1). The molecular weight marker (in kDa) is shown on the left; the expected location of HP1a, HP1ß and HP1? is indicated on the right. The WB analysis clearly demonstrates that the HP1a antibody specifically recognizes HP1a and not HP1ß and HP1?. Rabbit IgG Purified Hu
50 µg / €325.00
  OriGene Technologies, Inc.

CBX5 antibody

WB was performed on nuclear extracts from HeLa cells (20 ug) with the antibody against human HP1a, ß and ? diluted 1:1,000 in TBS-Tween containing 5% skimmed milk (Figure 1). The molecular weight marker (in kDa) is shown on the left; the expected location of HP1a, HP1ß and HP1? is indicated on the right. Rabbit IgG Purified Hu, Ms
50 µg / €325.00
  OriGene Technologies, Inc.

CBX5 antibody

WB was performed on nuclear extracts from HeLa cells (HeLa NE, 20 ug) using the antibody against hHP1 alpha diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. A molecular weight marker (in kDa) is shown on the left. The location of the three HP1 isoforms of interest is indicated on the right, demonstrating that the antibody only recognizes the alpha isoform. Rabbit IgG Purified Hu
0.1 ml / €325.00
  OriGene Technologies, Inc.

CBX5 antibody

WB was performed on nuclear extracts from HeLa cells (HeLa NE, 20 ug) using the antibody against HP1 alpha, beta and gamma diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. A molecular weight marker (in kDa) is shown on the left. The location of the three HP1 isoforms is indicated on the right. Rabbit IgG Purified Hu
0.1 ml / €325.00
  OriGene Technologies, Inc.

CBX5 antibody

Immunohistochemistry (IHC) analyzes of HP1-alpha antibody (Cat.-No.: AP06694PU-N) in paraffin-embedded human liver carcinoma tissue. Rabbit Aff - Purified Hu, Ms ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

CBX5 (88-102) antibody

Figure 1. Immunohistochemical analysis of Formalin-Fixed Paraffin-Embedded Human Uterus tissue using AP08784PU-N at 2.5 µg/ml. Goat IgG Aff - Purified Bear, Bov, Bt, Can, Elephant, Eq, Hst, Hu, Mky, Ms, Por, Prim, Rb, Rt, Sh E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

CBX5 pSer92 antibody

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HP1α (Phospho-Ser92) antibody AP55795PU-N (left)or the same antibody preincubated with blocking peptide (right). Rabbit Aff - Purified Hu P
0.1 ml / €340.00
  Acris Antibodies GmbH

CBX5 pSer92 antibody

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HP1α (Phospho-Ser92) antibody AP55795PU-N (left)or the same antibody preincubated with blocking peptide (right). Rabbit Aff - Purified Hu P
50 µl / €270.00
  Acris Antibodies GmbH

CBX5 antibody

CBX5 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

CBX5 antibody

Immunofluorescence of purified MaxPab antibody to CBX5 on HeLa cell. [antibody concentration 10 ug/ml] Mouse Serum Hu E(detection), ICC/IF, WB
50 µl / €370.00
  Abnova Taiwan Corp.

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