CCT1 / TCP1 antibody

Principal name

CCT1 / TCP1 antibody

Alternative names for CCT1 / TCP1 antibody

CCTA, CCT alpha, TCP-1-alpha, TCP1 alpha, T-complex protein 1 subunit alpha, Chaperonin containing T complex polypeptide 1 subunit 1

SwissProt ID

O94501 (Schpo), P11983 (Mouse), P12613 (Drome), P17987 (Human), P28480 (Rat), P28769 (Arath), P41988 (Caeel), Q32L40 (Bovin), Q3UE48 (Mouse)

Gene ID

6950 (TCP1), 21454 (Tcp1), 24818

Available reactivities

Bov (Bovine), Can (Canine), Hu (Human), Ms (Mouse), Por (Porcine), Rb (Rabbit), Rt (Rat), Ye (Yeast), Hst (Hamster), Sh (Sheep), Ce (C. elegans), Dros (Drosophila), GP (Guinea Pig), Mky (Monkey), S. cerevisiae (Saccharomyces cerevisiae), Frog, Chk (Chicken)

Available hosts

Rat, Mouse, Goat, Rabbit

Available applications

Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)

Background of CCT1 / TCP1 antibody

T-complex polypeptide-1 (TCP1) is a ~60 kDa protein constitutively expressed in almost all eukaryotic cells, and is up-regulated during spermatogenesis. It is found in the cytosol as a subunit of a hetero-oligomeric chaperone that is known to be involved in the folding of actin and tubulin. The family of proteins termed chaperonins act to recognize and stabilize polypeptide intermediates during folding, assembly and disassembly, and share many characteristics with Heat Shock Protein 70 (HSP 70) including high abundance, induction by environmental stress, and ATPase activity. The chaperonin family includes the mitochondrial HSP60, Escherichia coli GroEL, the plastid Rubisco-subunit binding protein, and the archaebacterial protein TF55. The TCP1 sequence shows nearly 40% identity to TF55, but only minimal similarity to HSP60 and GroEL.

General readings

Yaffe MB, Farr GW, Miklos D, Horwich AL, Sternlicht ML, Sternlicht H. TCP1 complex is a molecular chaperone in tubulin biogenesis. Nature. 1992 Jul 16;358(6383):245-8. PMID: 1630491

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CCT1 / TCP1 antibody

Immunoperoxidase of monoclonal antibody to TCP1 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3ug/ml] Mouse IgG1 2B2-D6 Purified Hu E, ICC/IF, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

CCT1 / TCP1 (C-term) antibody

TA302925 staining (1µg/ml) of Human Lung lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CCT1 / TCP1 antibody

WB of human lysates showing detection of ~60  kDa TCP1-alpha  protein using Anti-TCP1 , clone 23c.  Lane 1: MW ladder. Lane 2: A431 lysate, 20 µg. Lane3: HEK293 lysate, 20µg. Block: 5% milk + TBST 1hr at RT. Primary antibody: Rat monoclonal Anti-TCP1-alpha , clone 23c incubated for 60 min at RT. Secondary antibody: Goat Anti-Rat HRP antibody (1:1000) for 60 min at RT. Colour Development: TMB solution for 5 min at RT. Predicted/Observed size: ~60 kDa. Other band(s): ~90 kDa. Rat IgG2c 23c Purified Bov, Can, Hst, Ms, Rb, Rt, Sh WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CCT1 / TCP1 antibody

WB of human lysates showing detection of ~60  kDa TCP1-alpha  protein using Anti-TCP1 , clone 91a.  Lane 1: MW ladder. Lane 2: A431 lysate, 20 µg. Lane3: HEK293 lysate, 20µg. Block: 5% milk + TBST 1hr at RT. Primary antibody: Rat monoclonal Anti-TCP1-alpha , clone 91a incubated for 60 min at RT. Secondary antibody: Goat Anti-Rat HRP antibody (1:1000) for 60 min at RT. Colour Development: TMB solution for 5 min at RT. Rat IgG2a 91A Purified Bov, Can, Ce, Dros, GP, Hst, Hu, Mky, Ms, Por, Rb, Rt, S. cerevisiae WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CCT1 / TCP1 antibody

Western blot analysis of extracts of various cell lines, using TCP1 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

CCT1 / TCP1 antibody

HepG2 cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal TCP-1β antibody (clone F39P7F11). Proteins were visualized using a goat anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection. Mouse IgG2a F39P7F11 Purified Frog, Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CCT1 / TCP1 antibody

CCT1 / TCP1 Rat IgG2 23c (CTA-123) Purified Can, Chk, Hst, Ms, Rt ICC/IF, IP, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CCT1 / TCP1 antibody

CCT1 / TCP1 Rat IgG2 84a (CTA-184) Purified Ms IP, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CCT1 / TCP1 antibody

CCT1 / TCP1 Rat IgG1 91a (CTA-191) Purified Bov, Can, Hu, Ms, Por, Rb, Rt IP, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CCT1 / TCP1 antibody

CCT1 / TCP1 Rat IgG2 22b Purified Can, Chk, Hst, Ms, Rt E, IP, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CCT1 / TCP1 antibody

Western Blot analysis using Anti-TCPb [F39P7F11] Mouse IgG2 F39 P7 F11 Purified Frog, Hu, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CCT1 / TCP1 antibody

Western blot analysis of HeLa whole cell lysate (Lane 1) and HeLa heat stressed whole cell lysate (Lane 2) probed with Rat anti Mouse CCT alpha / TCP1 Antibody Cat.-No SM2173P followed by F(ab')2 Rabbit anti Rat IgG:HRP (Cat.-No SP1021HRP) Rat IgG2a 91a Purified Bov, Can, Hu, Ms, Por, Rb, Rt, Ye IP, WB
0.1 mg / €640.00
  Acris Antibodies GmbH

CCT1 / TCP1 antibody

CCT1 / TCP1 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

CCT1 / TCP1 antibody

TCP1 polyclonal antibody ( Cat # PAB6585 ) ( 10 µg/ml ) staining of paraffin embedded human testis. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining. Goat Hu E, P, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

CRISPR

Catalog No. Species Donor Vector  

TCP1 - human gene knockout kit via CRISPR

TCP1 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

TCP1 - human gene knockout kit via CRISPR

TCP1 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

TCP1 - human gene knockout kit via CRISPR

TCP1 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Tcp1 - mouse gene knockout kit via CRISPR

Tcp1 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Tcp1 - mouse gene knockout kit via CRISPR

Tcp1 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

CCT1 / TCP1 (transcript variant 1)

CCT1 / TCP1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

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