CCT1 / TCP1 antibody

Principal name

CCT1 / TCP1 antibody

Alternative names for CCT1 / TCP1 antibody

CCTA, CCT alpha, TCP-1-alpha, TCP1 alpha, T-complex protein 1 subunit alpha, Chaperonin containing T complex polypeptide 1 subunit 1

SwissProt ID

O94501 (Schpo), P11983 (Mouse), P12613 (Drome), P17987 (Human), P28480 (Rat), P28769 (Arath), P41988 (Caeel), Q32L40 (Bovin), Q3UE48 (Mouse)

Gene ID

6950 (TCP1), 21454 (Tcp1)

Available reactivities

Can (Canine), Chk (Chicken), Hst (Hamster), Ms (Mouse), Prim (Primate), Rt (Rat), Ye (Yeast), Bov (Bovine), Hu (Human), Por (Porcine), Rb (Rabbit), Sh (Sheep), Mky (Monkey), Ce (C. elegans), Dros (Drosophila), GP (Guinea Pig), S. cerevisiae (Saccharomyces cerevisiae), Frog

Available hosts

Rat, Mouse, Goat, Rabbit

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Frozen Sections (C), Flow Cytometry (F)

Background of CCT1 / TCP1 antibody

TCP1 beta is a subunit of a cytosolic hetero-oligomer chaperone (1,2) that is known to be involved in the folding of actin and tubulin. This protein is a member of the chaperonin family, which includes Escherichia coli GroEL, the mitochondrial heat-shock protein Hsp60, the plastid Rubisco-subunit-binding protein and the archaebacterial protein TF55. These chaperonins assist the folding of proteins upon ATP hydrolysis. Nine different subunits of TCP-1 containing chaperonin complexes from mammalian testis and seven different subunits of mouse F9 cells have been identified. The mouse TCP-1 subunits are between 531 and 545 residues in length. Their sequences are 2536% identical to one another, 2735% identical to that of TCP1 alpha and 3239% identical to that of the archaebacterial chaperonin, TF55. The genes for these subunits have been named Cctb, Cctg, Cctd, Ccte, Cctz and Ccth, which encode the CCTb, CCTg, CCTd, CCTe, CCTz, and CCTh subunits, respectively, of the `Chaperonin Containing TCP-1' (CCT). In bovine, CTT has also been referred to as `TRic', TCP-1 ring complex. All the CCT subunits contain motifs that are also shared by all other known chaperonins of prokaryotes and eukaryotic organelles, and that probably relate to their common ATPase function. It has been suggested that each CCT subunit has a specific, independent function, as they are highly diverged from each other but conserved from mammals to yeast. The expansion in the number of types of CCT subunit, compared with other chaperonins, has allowed CCT to carry out more complex functions that are required for the folding and assembly of highly evolved eukaryotic proteins.

General readings

Yaffe MB, Farr GW, Miklos D, Horwich AL, Sternlicht ML, Sternlicht H. TCP1 complex is a molecular chaperone in tubulin biogenesis. Nature. 1992 Jul 16;358(6383):245-8. PMID: 1630491

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CCT1 / TCP1 antibody

Immunoperoxidase of monoclonal antibody to TCP1 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3ug/ml] Mouse IgG1 2B2-D6 Purified Hu E, ICC/IF, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

CCT1 / TCP1 antibody

Immunohistochemistry: TCP1 alpha Antibody (EPR4081) [NBP1-40822] - Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-TCP1 alpha Rabbit IgG EPR4081 Hu ICC/IF, IP, P, WB
0.1 ml / €480.00
  Novus Biologicals Inc.

CCT1 / TCP1 (C-term) antibody

TA302925 staining (1µg/ml) of Human Lung lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Hu E, P, WB
20 µg / €200.00
  Acris Antibodies GmbH

CCT1 / TCP1 (C-term) antibody

TA302925 staining (1µg/ml) of Human Lung lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CCT1 / TCP1 antibody

WB of human lysates showing detection of ~60  kDa TCP1-alpha  protein using Anti-TCP1 , clone 23c.  Lane 1: MW ladder. Lane 2: A431 lysate, 20 µg. Lane3: HEK293 lysate, 20µg. Block: 5% milk + TBST 1hr at RT. Primary antibody: Rat monoclonal Anti-TCP1-alpha , clone 23c incubated for 60 min at RT. Secondary antibody: Goat Anti-Rat HRP antibody (1:1000) for 60 min at RT. Colour Development: TMB solution for 5 min at RT. Predicted/Observed size: ~60 kDa. Other band(s): ~90 kDa. Rat IgG2c 23c Purified Bov, Can, Hst, Ms, Rb, Rt, Sh WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CCT1 / TCP1 antibody

WB of human lysates showing detection of ~60  kDa TCP1-alpha  protein using Anti-TCP1 , clone 91a.  Lane 1: MW ladder. Lane 2: A431 lysate, 20 µg. Lane3: HEK293 lysate, 20µg. Block: 5% milk + TBST 1hr at RT. Primary antibody: Rat monoclonal Anti-TCP1-alpha , clone 91a incubated for 60 min at RT. Secondary antibody: Goat Anti-Rat HRP antibody (1:1000) for 60 min at RT. Colour Development: TMB solution for 5 min at RT. Rat IgG2a 91A Purified Bov, Can, Ce, Dros, GP, Hst, Hu, Mky, Ms, Por, Rb, Rt, S. cerevisiae WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CCT1 / TCP1 antibody

Western blot analysis of extracts of various cell lines, using TCP1 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

CCT1 / TCP1 antibody

HepG2 cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal TCP-1β antibody (clone F39P7F11). Proteins were visualized using a goat anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection. Mouse IgG2a F39P7F11 Purified Frog, Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CCT1 / TCP1 antibody

Western blot analysis of HeLa whole cell lysate (Lane 1) and HeLa heat stressed whole cell lysate (Lane 2) probed with Rat anti Mouse CCT alpha / TCP1 Antibody Cat.-No SM2173P followed by F(ab')2 Rabbit anti Rat IgG:HRP (Cat.-No SP1021HRP) Rat IgG2a 91a Purified Bov, Can, Hu, Ms, Por, Rb, Rt, Ye IP, WB
0.1 mg / €640.00
  Acris Antibodies GmbH

CCT1 / TCP1 antibody

CCT1 / TCP1 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

CCT1 / TCP1 antibody

TCP1 polyclonal antibody ( Cat # PAB6585 ) ( 10 µg/ml ) staining of paraffin embedded human testis. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining. Goat Hu E, P, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

CCT1 / TCP1 antibody

Immunohistochemistry: TCP1 Antibody [NB100-1400] - NB100-1400 (10ug/ml) staining of paraffin embedded Human Testis. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining. Goat IgG Aff - Purified Hu E, P, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

CCT1 / TCP1 antibody

Immunohistochemistry-Paraffin: TCP1 alpha Antibody [NBP1-88148] - Immunohistochemical staining of human testis shows distinct cytoplasmic positivity in seminiferus duct cells. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

CCT1 / TCP1 (transcript variant 1)

CCT1 / TCP1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

CCT1 / TCP1 (1-556, His-tag)

CCT1 / TCP1 Human Purified > 80 % E. coli
0.25 mg / €750.00
  Acris Antibodies GmbH

CCT1 / TCP1 (1-556, His-tag)

CCT1 / TCP1 Human Purified > 80 % E. coli
50 µg / €300.00
  Acris Antibodies GmbH

CCT1 / TCP1

CCT1 / TCP1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

CCT1 / TCP1

CCT1 / TCP1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

CCT1 / TCP1

CCT1 / TCP1 Human Purified
0.25 mg / €870.00
  Novus Biologicals Inc.

CCT1 / TCP1

CCT1 / TCP1 Human Purified
50 µg / €380.00
  Novus Biologicals Inc.

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