Bov (Bovine), Hu (Human), Ms (Mouse), Rb (Rabbit), Can (Canine), Chk (Chicken), Rt (Rat), Ze (Zebrafish), African clawed frog, Xen (Xenopus), Mky (Monkey), Eq (Equine), GP (Guinea Pig), Ye (Yeast)
Rat, Rabbit, Mouse
Immunoprecipitation (IP), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of CCT8 / TCP1 theta antibody
This gene encodes the theta subunit of the CCT chaperonin, which is abundant in the eukaryotic cytosol and may be involved in the transport and assembly of newly synthesized proteins. Altertive splicing results in multiple transcript variants of this gene. A pseudogene related to this gene is located on chromosome 1. [provided by RefSeq, Sep 2013].
Suzuki,Y., Gene 200 (1-2), 149-156 (1997)
Western blot analysis of HeLa whole cell lysate (Lane 1) and HeLa heat stressed whole cell lysate (Lane 2) probed with Rat anti CCT theta Cat.-No SM2017P followed by F(ab')2 Rabbit anti Rat IgG:HRP (Cat.-No SP1021HRP).
Western Blot: CCT8 Antibody (PK/13/72/8k) [NB100-66596] - Western blot analysis of HeLa whole cell lysate and HeLa heat stressed whole cell lysate probed with RAT ANTI CCT THETA followed by F2 Rabbit anti Rat IgG:HRP
Human HEK293T; CCT8 antibody - C-terminal region (ARP45837_P050) validated by WB using HEK 293T at 1:1,000 dilution for antibody samples and 1:10,000 for secondary antibodies.. CCT8 is strongly supported by BioGPS gene expression data to be expressed in Human HEK293T cells.; CCT8 antibody - C-terminal region (ARP45837_P050) in Human HEK293T cells using Western Blot
Human lymphoblastoid, Mouse brain; CCT8 antibody - C-terminal region (ARP45837_P050) validated by WB using human lymphoblastoid & mouse brain; CCT8 antibody - C-terminal region (ARP45837_P050) in Human lymphoblastoid, Mouse brain cells using Western Blot
Human Jurkat; WB Suggested Anti-CCT8 Antibody Titration: 0.2-1 ug/ml. Positive Control: Jurkat cell lysate.. CCT8 is strongly supported by BioGPS gene expression data to be expressed in Jurkat.; CCT8 antibody - C-terminal region (ARP45837_P050) in Human Jurkat cells using Western Blot
CCT8 Antibody (C-term) (Cat. #AP50830PU-N)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CCT8 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.