CCT8 / TCP1 theta antibody
Principal name
CCT8 / TCP1 theta antibody
Alternative names for CCT8 / TCP1 theta antibody
CCTQ, CCT theta, KIAA0002, NY-REN-15, T-complex protein 1 subunit theta, Renal carcinoma antigen NY-REN-15
Ncbi ID
NP_006576.2, 9606, NP_006576, NM_006585, NP_033970.3, NM_009840
Available reactivities
Available hosts
Available applications
Immunoprecipitation (IP), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), Enzyme Immunoassay (E)
Background of CCT8 / TCP1 theta antibody
- Kit Component:
- - KN302856G1, Cct8 gRNA vector 1 in pCas-Guide vector
- KN302856G2, Cct8 gRNA vector 2 in pCas-Guide vector
- KN302856D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Suzuki,Y., Gene 200 (1-2), 149-156 (1997)
Western blot analysis of HeLa whole cell lysate (Lane 1) and HeLa heat stressed whole cell lysate (Lane 2) probed with Rat anti CCT theta Cat.-No SM2017P followed by F(ab')2 Rabbit anti Rat IgG:HRP (Cat.-No SP1021HRP).
Human HEK293T; CCT8 antibody - C-terminal region (ARP45837_P050) validated by WB using HEK 293T at 1:1,000 dilution for antibody samples and 1:10,000 for secondary antibodies.. CCT8 is strongly supported by BioGPS gene expression data to be expressed in Human HEK293T cells.; CCT8 antibody - C-terminal region (ARP45837_P050) in Human HEK293T cells using Western Blot
Human lymphoblastoid, Mouse brain; CCT8 antibody - C-terminal region (ARP45837_P050) validated by WB using human lymphoblastoid & mouse brain; CCT8 antibody - C-terminal region (ARP45837_P050) in Human lymphoblastoid, Mouse brain cells using Western Blot
Human Jurkat; WB Suggested Anti-CCT8 Antibody Titration: 0.2-1 ug/ml. Positive Control: Jurkat cell lysate.. CCT8 is strongly supported by BioGPS gene expression data to be expressed in Jurkat.; CCT8 antibody - C-terminal region (ARP45837_P050) in Human Jurkat cells using Western Blot
CCT8 MaxPab polyclonal antibody. Western Blot analysis of CCT8 expression in human spleen.
Western Blot analysis of CCT8 expression in transfected 293T cell line (H00010694-T04) by CCT8 MaxPab polyclonal antibody.Lane 1: CCT8 transfected lysate(60.28 KDa).Lane 2: Non-transfected lysate.
CCT8 MaxPab polyclonal antibody. Western Blot analysis of CCT8 expression in Jurkat.
CCT8 Antibody (C-term) (Cat. #AP50830PU-N)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CCT8 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
CCT8 Antibody (C-term) (Cat. #AP50830PU-N) western blot analysis in K562 cell line lysates (35µg/lane).This demonstrates the CCT8 antibody detected the CCT8 protein (arrow).
Western blot analysis of TCP1 theta expression in H1299 (A); NIH3T3 (B); PC12 (C) whole cell lysates.
Sample (50 ug of whole cell lysate). A: Mouse brain. 7.5% SDS PAGE. TA308295 diluted at 1:5000.
Sample (30 ug of whole cell lysate). A: A431 . B: H1299. C: Hela. 7.5% SDS PAGE. TA308295 diluted at 1:1000.
Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft, using TCP-1 theta (TA308295) antibody at 1:500 dilution.
Western blot analysis of extracts from HeLa cells, using CCT8 antibody.The lane on the right is treated with the synthesized peptide.
Host: Rabbit; Target Name: CCT8; Sample Tissue: HT1080 Whole Cell lysates; Antibody Dilution: 1.0 ug/ml
WB Suggested Anti-CCT8 Antibody Titration: 0.2-1 ug/ml; Positive Control: Jurkat cell lysate.CCT8 is strongly supported by BioGPS gene expression data to be expressed in Jurkat
CCT8 antibody - C-terminal region validated by WB using human lymphoblastoid & mouse brain
CCT8 antibody - C-terminal region validated by WB using HEK 293T at 1: 1, 000 dilution for antibody samples and 1: 10, 000 for secondary antibodies.CCT8 is strongly supported by BioGPS gene expression data to be expressed in Human HEK293T cells
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA308295 | CCT8 / TCP1 theta antibody |
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| Rabbit Polyclonal antibody to TCP1 theta (chaperonin containing TCP1, subunit 8 (theta)) | |||||||
 |
Rabbit | IgG | Aff - Purified | Hu, Ms | P, WB |
0.1 ml /
€416.00
|
|
| OriGene Technologies, Inc. | |||||||
 +2 additional images |
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| TA312793 | CCT8 / TCP1 theta antibody |
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| Rabbit polyclonal anti-CCT8 antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA338927 | CCT8 / TCP1 theta antibody |
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| Rabbit Polyclonal Anti-CCT8 Antibody | |||||||
 |
Rabbit | IgG | Purified | Bov, Can, Eq, GP, Hu, Ms, Rb, Rt, Ze | WB |
50 µg /
€360.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA338928 | CCT8 / TCP1 theta antibody |
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| Rabbit Polyclonal Anti-CCT8 Antibody | |||||||
 |
Rabbit | IgG | Purified | Bov, Can, Eq, GP, Hu, Ms, Rt, Ye, Ze | WB |
50 µg /
€360.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|||||||
| AP50830PU-N | CCT8 / TCP1 theta (C-term) antibody |
||||||
 |
Rabbit | Ig | Aff - Purified | Hu | P, WB |
0.4 ml /
€370.00
|
|
| Acris Antibodies GmbH | |||||||
| +1 additional image |
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| SM2017P | CCT8 / TCP1 theta antibody |
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 |
Rat | IgG2a | PK/13/72/8k | Aff - Purified | Bov, Hu, Ms, Rb | IP, WB |
0.1 mg /
€660.00
|
| Acris Antibodies GmbH | |||||||
CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN302856 | Cct8 - mouse gene knockout kit via CRISPR |
||
 |
Mouse | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN302856BN | Cct8 - mouse gene knockout kit via CRISPR |
||
|
Mouse | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN302856LP | Cct8 - mouse gene knockout kit via CRISPR |
||
|
Mouse | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN302856RB | Cct8 - mouse gene knockout kit via CRISPR |
||
|
Mouse | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP310044 | CCT8 / TCP1 theta |
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| Recombinant protein of human chaperonin containing TCP1, subunit 8 (theta) (CCT8) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€438.00
|
|
| OriGene Technologies, Inc. | |||||
|
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Lysates
| Catalog No. | ||
|---|---|---|
| LY416546 | CCT8 overexpression lysate |
|
|
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Human Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RC210044L2V | Lenti ORF particles, CCT8 (mGFP-tagged) - Human chaperonin containing TCP1, subunit 8 (theta) (CCT8), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,082.00
|
|
| OriGene Technologies, Inc. | ||
| RC210044L1V | Lenti ORF particles, CCT8 (Myc-DDK tagged) - Human chaperonin containing TCP1, subunit 8 (theta) (CCT8) , 200 uL, >10^7 TU/mL |
|
|
200 µl /
€969.00
|
|
| OriGene Technologies, Inc. | ||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR208720L2V | Lenti ORF particles, Cct8 (GFP-tagged) - Mouse chaperonin containing Tcp1, subunit 8 (theta) (Cct8), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,216.00
|
|
| OriGene Technologies, Inc. | ||
| MR208720L1V | Lenti ORF particles, Cct8 (Myc-DDK-tagged) - Mouse chaperonin containing Tcp1, subunit 8 (theta) (Cct8), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,092.00
|
|
| OriGene Technologies, Inc. | ||
