CD10 / Neprilysin antibody

Principal name

CD10 / Neprilysin antibody

Alternative names for CD10 / Neprilysin antibody

MME, EPN, Neutral endopeptidase 24.11, Neutral endopeptidase, NEP, Enkephalinase, Atriopeptidase, CALLA, Common acute lymphocytic leukemia antigen

SwissProt ID

P07861 (Rat), P08473 (Human), P19621 (Pig), Q61391 (Mouse)

Gene ID

4311 (MME), 17380 (Mme)

Available reactivities

Hu (Human), Mky (Monkey), Bab (Baboon), Ms (Mouse), Rt (Rat), Por (Porcine)

Available hosts

Mouse, Rabbit, Rat

Available applications

Frozen Sections (C), Paraffin Sections (P), Flow Cytometry (F), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), Enzyme Immunoassay (E), Functional assay (FN)

Background of CD10 / Neprilysin antibody

The mammalian neprilysin (NEP) family members are typically type II membrane endopeptidases responsible for the activation/inactivation of neuropeptides and peptide hormones. The substrate specificity and subcellualar localization of the 7 mammalian NEPs contribute to their functional heterogeneity. Proteolytic enzymes constitute around 2% of the human genome and are several stages of cell development from fertilization to cell death as a result of apoptosis. The identification of many novel proteases from genome-sequencing programs has suggested them as potential new therapeutic targets. In addition, several well-characterized metallopeptidases were recently shown to possess new biological roles in neuroinflammation and neurodegeneration. Neprilysin, a zinc-metalloendopeptidase, located on chromosome 3q21-q27 contains 24 exons and plays important role in the physiology and pathology of many diseases such as hypertension, cancer and Alzheimer’s disease (AD). In AD the deposition of amyloid beta peptides (Abeta) in brain form plaques that are hallmark of AD codition. Neprilysin is the major Abeta degrading enzyme and a reduction in Neprilysin activity contribute to the extent of plaque formation in AD by increasing the steady state levels of Abeta (1). The levels of Abeta in the central nervous system are regulated by several catabolic proteases, including insulysin (IDE), BACE1, BACE2, presenilin1, presenilin2, gamma secretase and neprilysin (NEP). Gleevec, a known tyrosine kinase inhibitor, has been shown to lower Abeta secretion, and it is considered a potential basis for novel therapies for Alzheimer’s disease. In vitro treatment of cells with Gleevec elevates neprilysin levels that caused an increased Abeta degradation (2).
Neutral endopeptiddase, Neprilysin is a major enzyme involved in the degradation and metablic inactivation of a number of biological peptides including enkephlins, substance P, bradykinin, incretin hormone glucagon like peptide 1 and arterial natriuretic peptides. Neprilysin hydrolyze the brain derived natriuretic peptide (BNP) but not the structurally related arterial or and C-type natriuretic peptides which are important cardioprotective hormones with essential function in sodium excretion, water balance, and blood pressure regulation. The Neprilysin is a 750 amino acid protein with predicted molecular weight of 86 kDa but as a result of abundant post translational modifications, specially glycosylation, the brain Neprilysin migrates between 98-110 kDa on denaturing SDS-PAGE. The Neprilysin is a membrane bound glycoprotein with a short N-termainl cytoplasmic tail, and TMD and a large C-terminal extracellular catalytic domain comprise of a zinc binding motif that also facilitates the small hydrophobic peptides. The Neprilysin is ubiquitously expressed in many tissues including intestinal BBM, kidney epithelial cells, neutrophils, thymocytes, lung, testis and in neuronal plasma brain (3).

General readings

1. CD10/Neutral Endopeptidase 24.11 Hydrolyzes Bombesin-like Peptides and Regulates the Growth of Small Cell Carcinomas of the Lung. Proc Natl Acad Sci USA 1991, Dec1;88(23):10662-6. Shipp, MA, Tarr, GE; Chen, CY; Switzer, SN; Hersh, LB; Stein,H; Sunday, ME; Reinherz, EL.
2 Induction of Isotype Switching and Ig Production by CD5+ and CD10+ Human Fetal B Cells. JI, 1992, Jun1;148(11):3398-404. Punnonen, J; Aversa, GG;Vandekerckhove, B; Roncarolo, MG; de Vries, JE.
3. Murine Common Acute Lymphoblastic Leukemia Antigen (CD10 Neutral Endopeptidase 24.11). Molecular Characterization, Chromosomal Localization and Modeling of the Active Site. JI, 1992, May1;148(9):2817-25. Chen, CY; Salles, G; Seldin, MF; Kister, AE; Reinherz, EL; Shipp, MA.
4. Childhood Acute Leukemia with t(11;19) (q23;p13). SO. Leukemia, 1991, Dec;5(12):1064-8. Hudson, MM; Raimondi, SC; Behm, FG; Pui, CH.
5. CD10 (CALLA)/Neutral Endopeptidase 24.11 Modulates Inflammatory Peptide-Induced Changes in Neutrophil Morphology, Migration and Adhesion Proteins and is itself Regulated by Neutrophil Activation. Blood, 1991, Oct. 1;78(7):1834-41. Shipp, MA; Stefano, GB; Switzer, SN; Griffin, JD; Reinherz, EL.
6. Functional Properties of CD19+ B Lymphocytes Positively Selected from Buffy Coats by Immunomagnetic Separation. Funderud, S., Erikstien, B., Asheim, H.C., Nustad ,K., Stokke, T., Blomhoff, H.K., Holte, H., Smeland, E.B.. Eur. J. Immunol. 1990 Ja;20(1):201-6
7. Thymic B Cells from Myasthenia Gravis Patients are Activated B Cells. Phenotypic and Functional Analysis. Leprince, C., Cohen-Kaminsky, S., Berrih-Aknin, S., Vernet-Der Garabedian, B., Treton, D., Galanaud, P., Richard, Y., J. mmunol. 1990 Oct., 145(7):2115-22.
8. Prognostic Significance of CD34 Expression in Childhood B Precursor Acute Lymphocytic Leukemia: A Pediatric Onocology Group Study. Borowitz, M.J., Shuster, J.J., Civin, C.I., Carrol, A.J., Look, A.T., Behm, F.G., Land, V.J., Pullen, D.J., Crist, W.M.. J. Clin. Onol. 1990 Au;8(8):1389-98.
9. Biphenotypic Acute Leukemia in Adults. Sulak, L.E., Clare, C.N., Morale, B.A., Hansen, K.L., Montiel, M.M.. Am. J. Clin. Path. 1990 Ju;94(1):54-8.
10. Intersection of the Complement and Immune Systems: A Signal Transduction Complex of the B Lymphocyte Containing Complement Receptor type 2 and CD19. Matsumoto, A.K., Kopicky-Burd , J., Carter, R.H., Tuveson, D.A., Tedder, T.F., Fearon, D.T., J. Exp. Med. 1991 Jan. 173(1):55-64.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD10 / Neprilysin antibody

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-MME mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504507) Mouse IgG2a OTI3D11 Purified Hu P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

CD10 / Neprilysin antibody

Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-MME Rabbit Polyclonal antibody. (TA590055) Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD10 / Neprilysin antibody

Immunohistochemistry-Paraffin: CD10 Antibody (2A1H5E1) [NBP1-47356] - Immunohistochemical analysis of paraffin-embedded lung cancer tissues using SOX9 Rabbit pAb with DAB staining. Mouse IgG2b 2A1H5E1 Aff - Purified Hu E, P
0.1 mg / €380.00
  Novus Biologicals Inc.

CD10 / Neprilysin (N-term) antibody

Formalin-Fixed, Paraffin-Embedded Human  tonsil tissue (4 μm) stained with anti-CD10 antibody (DB 057) antibody shows strong positive immunostaining of germinal centre cells. Kindly performed and provided by Katarína Poliaková, MD and Ľubomír Straka, MD, Ph. D., from Clinical Pathology Prešov, Ltd., Prešov, Slovak republic. Rabbit IgG G27-P Aff - Purified Hu C, P
0.1 ml / €310.00
  DB Biotech, spol. s r.o.

Sample sizes available

CD10 / Neprilysin (N-term) antibody

Formalin-Fixed, Paraffin-Embedded Human  tonsil tissue (4 μm) stained with anti-CD10 antibody (DB 057) antibody shows strong positive immunostaining of germinal centre cells. Kindly performed and provided by Katarína Poliaková, MD and Ľubomír Straka, MD, Ph. D., from Clinical Pathology Prešov, Ltd., Prešov, Slovak republic. Rabbit IgG G27-P Aff - Purified Hu C, P
0.2 ml / €420.00
  DB Biotech, spol. s r.o.

Sample sizes available

CD10 / Neprilysin (N-term) antibody

Formalin-Fixed, Paraffin-Embedded Human  tonsil tissue (4 μm) stained with anti-CD10 antibody (DB 057) antibody shows strong positive immunostaining of germinal centre cells. Kindly performed and provided by Katarína Poliaková, MD and Ľubomír Straka, MD, Ph. D., from Clinical Pathology Prešov, Ltd., Prešov, Slovak republic. Rabbit IgG G27-P Aff - Purified Hu C, P
0.5 ml / €540.00
  DB Biotech, spol. s r.o.

Sample sizes available

CD10 / Neprilysin (N-term) antibody

Formalin-Fixed, Paraffin-Embedded Human  tonsil tissue (4 μm) stained with anti-CD10 antibody (DB 057) antibody shows strong positive immunostaining of germinal centre cells. Kindly performed and provided by Katarína Poliaková, MD and Ľubomír Straka, MD, Ph. D., from Clinical Pathology Prešov, Ltd., Prešov, Slovak republic. Rabbit IgG G27-P Aff - Purified Hu C, P
1 ml / €840.00
  DB Biotech, spol. s r.o.

Sample sizes available

CD10 / Neprilysin antibody

Human kidney stained with anti-CD10 antibody AP15412PU Rabbit Purified Hu P
0.5 ml / €360.00
  Acris Antibodies GmbH

CD10 / Neprilysin antibody

Human kidney stained with anti-CD10 antibody AP15412PU Rabbit Purified Hu P
1 ml / €520.00
  Acris Antibodies GmbH

CD10 / Neprilysin antibody

Human kidney stained with anti-CD10 antibody AP15412PU Rabbit Purified Hu P
0.1 ml / €190.00
  Acris Antibodies GmbH

CD10 / Neprilysin antibody

Staining of normal human peripheral blood cells with 0.25 ug of Mouse IgG2b Isotype Control Purified (open histogram) or 0.125  ug of Anti-Human CD10 Purified  (filled histogram) followed by Anti-Mouse IgG  Biotin and Streptavidin PE .  Cells in the granulocyte gate were used for analysis. Mouse eBioCB-CALLA (CB-CALLA) Purified Hu F
0.1 mg / €120.00
  OriGene Technologies, Inc.

CD10 / Neprilysin antibody

Predicted band size: 86 kDa . Positive control: 293T cell lysate. Recommended dilution: 1/500-2000 Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD10 / Neprilysin antibody

Predicted cell location: Cytoplasm. Positive control: Human brain tissue. Recommended dilution: 1/50-200  The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using MME antibody at dilution 1/85, on the right is treated with the synthetic peptide. (Original magnification: x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD10 / Neprilysin (Center) antibody

MME Antibody (C-term) (Cat.# TA324359) western blot analysis in LNCaP cell line lysates (35ug/lane).This demonstrates the MME antibody detected the MME protein (arrow). Rabbit Ig Aff - Purified Hu, Ms P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

CD10 / Neprilysin (C-term) antibody

Western blot analysis of Neprilysin Antibody (C-term) (Cat. #TA324923) in A2058,A375,Ramos cell line lysates (35ug/lane). EPN (arrow) was detected using the purified Pab. Rabbit Hu P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

CD10 / Neprilysin antibody

Western blot analysis of lysates from Ramos cell line,mouse kidney and rat kidney tissue (from left to right),using Neprilysin Antibody (Center)(Cat. #TA324924).TA324924 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane. Rabbit IgG Purified Hu, Ms, Rt ICC/IF, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

CD10 / Neprilysin antibody

Immunohistochemistry staining of Paraffin Kidney, tonsil tissue by CD10 antibody (dilution: 1:10 - 1:50; visualization of staining: Cytoplasmic, membranous) Mouse IgG1 56C6 Purified Hu P
1 ml / €1,000.00
  OriGene Technologies, Inc.

CD10 / Neprilysin antibody

Western blot analysis of extracts of various cell lines, using MME antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD10 / Neprilysin antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-MME mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808300)(1:150) Mouse IgG2b OTI2A6 Purified Hu P
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD10 / Neprilysin antibody

CD10 / Neprilysin Rabbit IgG K16-P FITC Hu F
100 Tests / €560.00
  DB Biotech, spol. s r.o.

Sample sizes available

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