CD100 / SEMA4D antibody

Principal name

CD100 / SEMA4D antibody

Alternative names for CD100 / SEMA4D antibody

Semaphorin-4D, SEMAJ, BB18, A8, GR3

SwissProt ID

O09126 (Mouse), Q90665 (Chick), Q92854 (Human), Q92854-2

Gene ID

10507 (SEMA4D)

Available reactivities

Hu (Human), Mky (Monkey), Ms (Mouse)

Available hosts

Mouse, Rabbit, Rat

Available applications

Flow Cytometry (F), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E)

Background of CD100 / SEMA4D antibody

The Semaphorins are a large family of phylogenetically conserved proteins that play a pivotal role in maintaining homeostasis in the immune system. Twenty members of this family have been identified and categorized into eight subclasses based on sequence similarity and distinctive structural features. CD100, also known as Sema4D, is a 150 kDa transmembrane class IV semaphorin. Studies have shown that CD100 can induce monocyte migration, T-cell activation, and B-cell survival, as well as T/B cell and T/DC “cooperation”. The CD100 precursor contains 862 amino acids, including a 21 a.a. signal sequence, a 713 a.a. extracellular domain, a 21 a.a. transmembrane sequence, and a 107 a.a cytoplasmic region. The extracellular sequence contains several structural features, including a 479 a.a “sema” domain, a 79 a.a. Ig-like sequence, and a 52 a.a “Plexin-type repeat”.

General readings

Knapp, W. et al., Leucocyte typing IV, p 391 and p 396. Oxford Univ. Press. 1989

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD100 / SEMA4D antibody

  Mouse Anti-Human CD100 (SEMA4D) Purified (25 ug)  
Staining of normal human peripheral blood cells with 0.25 ug of Mouse IgM Isotype Control Purified, (open histogram) or Anti-Human CD100 (SEMA4D) Purified (filled histogram) followed by Anti-Mouse IgM PE . Cells in the lymphocyte gate were used for analysis. Mouse eBio133-1C6 (133-1C6) Purified Hu E, F, IP
25 µg / €130.00
  OriGene Technologies, Inc.

CD100 / SEMA4D antibody

  Rabbit Polyclonal Semaphorin 4D/CD100 Antibody  
Western Blot: SEMA4D Antibody [TA336502] - WB analysis of transfected CHO cells with SEMA4D antibody used at a dilution of 1:500. Rabbit Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD100 / SEMA4D antibody

  Rabbit Polyclonal Semaphorin 4D/CD100 Antibody  
Western Blot: SEMA4D Antibody [TA336503] - Analysis of SEMA4D protein in transfected CHO cells using TA336503 at  a dilution of 1:500. Rabbit Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD100 / SEMA4D antibody

  Mouse Monoclonal anti-CD100 Antibody  
Human peripheral blood lymphocytes stained with purified A8, followed by anti-mouse IgG FITC Mouse A8 Purified Hu F, IP, WB
25 µg / €248.00
  OriGene Technologies, Inc.

CD100 / SEMA4D antibody

Flow cytometric analysis of mouse CD100/Sema4D expression on EL-4 cells. Open histogram indicates the reaction of isotypic control to the cells. Shaded histogram indicates the reaction of AM26488AF-N to the cells. Rat IgG1 MMA-12 Azide Free Ms F
0.1 mg / €320.00
  Acris Antibodies GmbH

CD100 / SEMA4D antibody

Flow cytometric analysis of mouse CD100/Sema4D expression on mouse CD100/Sema4D transfected cells (right) and parental cells (left). Open histograms indicate the reaction of isotypic control to the cells. Shaded histograms indicate the reaction of AM26488FC-N to the cells. Rat IgG1 MMA-12 FITC Ms F
0.1 ml / €340.00
  Acris Antibodies GmbH

CD100 / SEMA4D antibody

CD100 / SEMA4D Mouse IgM 133.1G6 Purified Hu IP
0.1 mg / €310.00
  Acris Antibodies GmbH

CD100 / SEMA4D antibody

Peripheral Human lymphocytes stained with Mouse Anti Human CD100-APC (SM1174APC). Mouse IgG1 A8 APC Hu, Mky F
100 Tests / €440.00
  Acris Antibodies GmbH

CD100 / SEMA4D antibody

CD100 / SEMA4D Mouse IgG1 A8 FITC Hu, Mky F
0.1 mg / €390.00
  Acris Antibodies GmbH

CD100 / SEMA4D antibody

CD100 / SEMA4D Mouse IgG1 A8 FITC Hu, Mky F
25 µg / €190.00
  Acris Antibodies GmbH

CD100 / SEMA4D antibody

CD100 / SEMA4D Mouse IgG1 A8 Low Endotoxin Hu, Mky F, IP, WB
0.5 mg / €560.00
  Acris Antibodies GmbH

CD100 / SEMA4D antibody

CD100 / SEMA4D Mouse IgG1 A8 Purified Hu, Mky F, IP, WB
0.2 mg / €440.00
  Acris Antibodies GmbH

CD100 / SEMA4D antibody

CD100 / SEMA4D Mouse IgG1 A8 Purified Hu, Mky F, IP, WB
0.1 mg / €280.00
  Acris Antibodies GmbH

CD100 / SEMA4D antibody

CD100 / SEMA4D Mouse IgG1 A8 Purified Hu, Mky F, IP, WB
25 µg / €180.00
  Acris Antibodies GmbH

CD100 / SEMA4D antibody

SM1174R staining of human peripheral blood lymphocytes. Mouse IgG1 A8 PE Hu, Mky F
100 Tests / €480.00
  Acris Antibodies GmbH

CD100 / SEMA4D antibody

SM1174R staining of human peripheral blood lymphocytes. Mouse IgG1 A8 PE Hu, Mky F
25 Tests / €220.00
  Acris Antibodies GmbH

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

CD100 / SEMA4D (transcript variant 1)

  Recombinant protein of human sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D (SEMA4D), transcript variant 1  
CD100 / SEMA4D Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

CD100 / SEMA4D (transcript variant 1)

  Purified recombinant protein of Human sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D (SEMA4D), transcript variant 1  
CD100 / SEMA4D Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
10 µg / €139.00
  OriGene Technologies, Inc.

CD100 / SEMA4D (transcript variant 1)

  Purified recombinant protein of Human sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D (SEMA4D), transcript variant 1.  
CD100 / SEMA4D Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
CHO cells

Regular Price: 20 µg / €399.00

Special Price: 20 µg / €239.00

  OriGene Technologies, Inc.

CD100 / SEMA4D (sCD100)

CD100 / SEMA4D Human > 97 % pure by SDS-PAGE gel and HPLC analyses. CHO cells
20 µg / €230.00
  Acris Antibodies GmbH

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