CD103 antibody

Principal name

CD103 antibody

Alternative names for CD103 antibody

Integrin alpha-E, Integrin alpha-IEL, HML-1 antigen, ITGAE, Mucosal lymphocyte 1 antigen

SwissProt ID

O88341 (Rat), P38570 (Human), Q60677 (Mouse)

Gene ID

3682 (ITGAE), 16407, 2209 (FCGR1A)

Available reactivities

Hu (Human), Mky (Monkey), Rt (Rat), Ms (Mouse), Prim (Primate)

Available hosts

Mouse, Rat, Hamster, Rabbit

Available applications

Frozen Sections (C), Immunoprecipitation (IP), Flow Cytometry (F), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Functional assay (FN), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of CD103 antibody

CD103/Integrin alpha E is a new member of the integrin series of adhesion molecules containing the beta7 subunit. This antigen defines a developmentally important subset of T cells, namely mucosal T cells including all IEL (intraepithelial lymphocytes) and approx. 20% of lamina propria T cells. Expression of CD103 is more restricted outside these mucosal organs, appearing at lower levels on T cell subsets of the lymph node, dendritic epidermis and periphery. In non-epithelial CD103+ T cells there is a bias toward expression on CD8+ cells. Integrin alpha E is a heterodimer comprising a 150kDa alpha chain and a 120kDa beta chain. Integrin alpha E is expressed by approximately 1% of peripheral blood lymphocytes, activated T lymphocytes and by hairy cell leukaemia cells. The antigen is also expressed by intraepithelial lymphocytes. This antibody is reported to have signal inducing activity in a redirected lysis assay and to co stimulate IEL and CD8+ lymph node cells in conjunction with anti TCR in vitro, which runs counter to the usual inhibition of CTL by anti integrins. The property of costimulation is not unique to the mouse since the combination of anti human alpha IEL (intraepithelial lymphocytes) plus anti TCR will produce similar proliferation in human cells, with the exception that CD8+ cells are not stimulated.

General readings

1. Flenghi L, Spinozzi F, Stein H, Krushwitz M, Pileri S, Falini B. LF61: a new monoclonal antibody directed against a trimeric molecule (150 kDa, 125 kDa, 105 kDa) associated with hairy cell leukaemia. Br J Haematol. 1990 Dec;76(4):451-9. PubMed PMID: 2265107.
2. Falini B, Pileri SA, Flenghi L, Liberati M, Stein H, Gerli R, et al. Selection of a panel of monoclonal antibodies for monitoring residual disease in peripheral blood and bone marrow of interferon-treated hairy cell leukaemia patients. Br J Haematol. 1990 Dec;76(4):460-8. PubMed PMID: 1702309.
3. Ling KL, Dulphy N, Bahl P, Salio M, Maskell K, Piris J, et al. Modulation of CD103 expression on human colon carcinoma-specific CTL. J Immunol. 2007 Mar 1;178(5):2908-15. PubMed PMID: 17312135. 
4. Hadley, G.A. et al. (2001) CD103+ CTL accumulate within the graft epithelium during clinical renal allograft rejection. Transplantation. 72 (9): 1548-55.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD103 antibody

Staining of mouse lymph node cells with Anti-Mouse CD8a APC and 0.5 ug of Armenian Hamster IgG Isotype Control Purified (left) or 0.5 ug of Anti-Mouse CD103 (Integrin alpha E) Purified (right) followed by Anti-Armenian Hamster IgG Biotin and Streptavidin FITC .  Cells in the lymphocyte gate were used for analysis. Hamster 2E7 Purified Ms F, IP, P
50 µg / €120.00
  OriGene Technologies, Inc.

CD103 antibody

Staining of 3-day PHA-stimulated normal human peripheral blood cells with 0.25 ug of Mouse IgG1 kappa Isotype Control Purified (open histogram) or 0.25 ug of Anti-Human CD103 (Integrin alpha E) Purified (filled histogram) followed by Anti-Mouse IgG FITC .  Total viable cells were used for analysis. Mouse B-Ly7 Purified Hu F, P
0.1 mg / €120.00
  OriGene Technologies, Inc.

CD103 antibody

Western blot analysis of Integrin alpha-E expression in HepG2 cells Rabbit IgG Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD103 antibody

Immunohistochemical staining of human kidney shows strong membranous positivity in tubular cells.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only. Rabbit IgG Purified Hu E, ICC/IF, P
50 µg / €325.00
  OriGene Technologies, Inc.

CD103 antibody

CD103 Mouse IgG1 AX.14 FITC Hu F
100 Tests / €270.00
  Acris Antibodies GmbH

CD103 antibody

CD103 Mouse IgG1 AX.14 Purified Hu F
0.1 mg / €240.00
  Acris Antibodies GmbH

CD103 antibody

CD103 Mouse IgG1 AX.14 PE Hu F
100 Tests / €300.00
  Acris Antibodies GmbH

CD103 antibody

Immunofluorescent Staining: BALB/c mesenteric lymph node cells were first labeled with Hamster Anti-Mouse CD103-Biotin, and then double-stained with streptavidin-FITC and Rat Anti-Mouse CD8a-PE (AM08023RP-N or AM08023RP-S). Small lymphocytes were gated and analyzed on a FACScan(TM) flow cytometer (BDIS, San Jose, CA). Amount Used: < / = 0.1 µg/10e6 cells. Hamster IgG 2E7 Biotin Ms F
0.5 mg / €380.00
  Acris Antibodies GmbH

CD103 antibody

CD103 Hamster IgG 2E7 FITC Ms F
0.5 mg / €410.00
  Acris Antibodies GmbH

CD103 antibody

CD103 Hamster IgG 2E7 Low Endotoxin Ms C, F, FN, IP
0.5 mg / €380.00
  Acris Antibodies GmbH

CD103 antibody

CD103 Hamster IgG 2E7 Purified Ms C, F, FN, IP
0.5 mg / €370.00
  Acris Antibodies GmbH

CD103 antibody

CD103 Hamster IgG 2E7 PE Ms F
0.1 mg / €280.00
  Acris Antibodies GmbH

CD103 antibody

CD103 Mouse IgG1 Ber-ACT8 APC Hu, Prim F
100 Tests / €290.00
  Acris Antibodies GmbH

CD103 antibody

CD103 Mouse IgG1 Ber-ACT8 FITC Hu, Prim F
100 Tests / €260.00
  Acris Antibodies GmbH

CD103 antibody

CD103 Mouse IgG1 Ber-ACT8 Aff - Purified Hu, Prim C, F, IP, WB
0.1 mg / €210.00
  Acris Antibodies GmbH

CD103 antibody

CD103 Mouse IgG1 Ber-ACT8 PE Hu, Prim F
100 Tests / €290.00
  Acris Antibodies GmbH

CD103 antibody

Representative Data
Staining with clone B-ly7 (anti-CD103) monoclonal antibody is illustrated by flow cytometry analysis using a spleen cell suspension from a HCL patient. Direct staining was performed using 10 µl of the FITC-conjugated antibody and 100 µl spleen cell suspension. Mouse IgG1 B-ly7 FITC Hu F
100 Tests / €370.00
  Acris Antibodies GmbH

CD103 antibody

Representative Data
Staining with clone B-ly7 (anti-CD103) monoclonal antibody is illustrated by flow cytometry analysis using a spleen cell suspension from a HCL patient. Direct staining was performed using 10 µl of the FITC-conjugated antibody and 100 µl spleen cell suspension. Mouse IgG1 B-ly7 PerCP-Cy5.5 Hu F
100 Tests / €450.00
  Acris Antibodies GmbH

CD103 antibody

Representative Data
Staining with clone B-ly7 (anti-CD103) monoclonal antibody is illustrated by flow cytometry analysis using a spleen cell suspension from a HCL patient. Direct staining was performed using 10 µl of the FITC-conjugated antibody and 100 µl spleen cell suspension. Mouse IgG1 B-ly7 Aff - Purified Hu C, F
1 ml / €260.00
  Acris Antibodies GmbH

CD103 antibody

Representative Data
Staining with clone B-ly7 (anti-CD103) monoclonal antibody is illustrated by flow cytometry analysis using a spleen cell suspension from a HCL patient. Direct staining was performed using 10 µl of the FITC-conjugated antibody and 100 µl spleen cell suspension. Mouse IgG1 B-ly7 PE Hu F
100 Tests / €430.00
  Acris Antibodies GmbH

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