Integrins are heterodimers composed of alpha and beta subunits, that are noncovalently associated transmembrane glycoprotein receptors. Different combinations of alpha and beta polypeptides form complexes that vary in their ligand-binding specificities. Integrins mediate cell-matrix or cell-cell adhesion, and transduced signals that regulate gene expression and cell growth. This gene encodes the integrin beta 4 subunit, a receptor for the laminins. Integrin b4 (CD104) associates with integrin α6 (CD49f) forming the α6/β4 (CD49f/CD104) heterodimer. CD104 is expressed on epithelial cells (especially on the proliferative basal layer epithelial cells in skin), endothelial cells, Schwann cells, certain tumor cells and a subset of pre-T cells. CD49f/CD104 is an adhesion receptor for laminins (especially laminin 5) and keratin filaments and is involved in the regulation of hemidesmosome formation and of cell proliferation and activation. CD104 is likely to play a pivotal role in the biology of invasive carcinoma. Mutations in this gene are associated with epidermolysis bullosa with pyloric atresia. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
1. Kennel, S.J. et al. (1990) Second generation monoclonal antibodies to the human integrin alpah6 beta4. Hybridoma 9: 243-254.
Cryostat section of human tonsil stained with anti-CD104 antibody (SM1205P/PT).
Western blot analysis of A-431 cells serum starved overnight (lane 1) and treated with pervanadate (1mM) for 30 min (lane 2). The blots were probed with ITGB4 monoclonal antibody, clone M126 ( Cat # MAB1371 ).
Western blot analysis of A-431 cells serum starved overnight (lane 1) and treated with pervanadate (1mM) for 30 min (lane 2). The blots were probed with ITGB4 (phospho Y1494) polyclonal antibody ( Cat # PAB7915 ).
Western blot analysis of A-431 cells serum starved overnight (lane 1) and treated with pervanadate (1mM) for 30 min (lane 2). The blots were probed with ITGB4 (phospho Y1526) polyclonal antibody ( Cat # PAB7916 ).
Staining of the A549 cell line with 0.5 µg of Purified Rat IgG2b Isotype Control (open histogram) or 0.5 µg of Purified anti-Human CD104 antibody (439-9B) (colored histogram) followed by Biotin Anti-Rat IgG and Sav-PE. Total viable cells were used for analysis.
1X10^6 HeLa cells were stained with 0.2ug Integrin beta-4 antibody (21738-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
Immunohistochemistry on frozen section of human skin showing strong staining of basal membrane (e.g. cells of sebaceous gland and sweat gland and cells forming the hair in the hair shaft) and connective tissue; no staining of muscle cells.
Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti ITGB4 Polyclonal Antibody, Unconjugated (bs-4115R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat skin tissue tissue labeled with Anti PHOSPHO-ITGB4(TYR1494) Polyclonal Antibody,Unconjugated (bs-5455R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Lane 1: mouse heart lysates Lane 2: mouse brain lysates probed with Anti phospho-ITGB4(Tyr1526) Polyclonal Antibody, Unconjugated (bs-5456R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 197kD. Observed band size: 197kD
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using ITGB4 (Phospho-Tyr1510) antibody AP55727PU-N (left)or the same antibody preincubated with blocking peptide (right).
Immunohistochemical analysis of CD104 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.